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Objective To study the protective function of estradiol after cerebral ischemia reperfusion and its effect on the expression of GDNF.

目的 探讨大鼠局灶脑缺血再灌注后雌二醇的保护作用及其对胶质细胞源性神经生长因子表达的影响。

TNF-α that activated retinal glial cells may play an important role in glaucomatous retinal ganglion cell damage.

在慢性高眼压情况中,活化的视网膜胶质细胞来源的TNF-α可能在神经节细胞损伤中发挥重要作用。

Objective To study the effects of ciliary neurotrophic factor on the expression of glia fibrillary acidic protein mRNA and on the functional recovery after spinal cord injur in rats.

目的 研究大鼠脊髓损伤后睫状神经营养因子是否能够影响胶质纤维酸性蛋白的表达和脊髓损伤后大鼠功能的恢复情况。

Besides hemal sex migraine, cause the cause that have a headache a lot of, if abscess of all sorts of sexual disease, encephalitis, meningitis, head is affected inside skull; All sorts of cerebral blood-vessel pathological changes, if cerebral block, cerebral hemorrhage, arachnoid haemorrhage finishing speech, ventricle bleeds, Hypertensive Cerebral disease; A pathological change is taken inside skull, if colloid tumour mixes meningeal tumour, head,transfer tumor; All sorts of heads are traumatic if defeat of cerebral concussion, head cracks injury, putamen,issue haematoma; The waist is worn cause low skull pressure head painful; All sorts of neuralgic; The headache of disease of facial features division and infection, toxic, heatstroke and nerve function all can be caused mussily have a headache.

除了血管性偏头痛外,引起头痛的原因很多,如颅内感染性疾病、各种脑炎、脑膜炎、脑脓肿;各种脑血管病变,如脑梗塞、脑出血、蛛网膜下腔出血、脑室出血、高血压脑病等;颅内占位病变,如脑膜瘤、脑胶质瘤和转移肿瘤;各种脑外伤如脑震荡、脑挫裂伤、硬膜下血肿;腰穿引起低颅压头痛;各种神经痛;五官科疾病的头痛以及感染、中毒、中暑和神经功能紊乱均可引起头痛。

Cerebral hemorrhage rat models were established via injection of autologous arterial blood in caudate nucleus. Two days after modeling, 5 μL BrdU-labeled human embryonic NSCs suspension was transplanted at four points surrounding hematoma cavity in the rats. After 1 and 2 weeks, rats were sacrificed. Adjacent sections were doubly stained by BrdU/microtubule-associated protein 2 (MAP-2) and BrdU/glial fibrillary acidic protein.

通过注射自体动脉血到尾状核制作大鼠脑出血模型,出血后2 d将标有5'-溴脱氧尿嘧啶的人胚神经干细胞悬液移植到血肿腔周围的4点,1,2周后处死大鼠,相邻脑组织切片行5'-溴脱氧尿嘧啶/微管相关蛋白2和5'-溴脱氧尿嘧啶/胶质纤维酸性蛋白免疫组织化学双染。

METHODS: Cerebral cortex cells of 8-week aborted human fetus were harvested and cultured in vitro to obtain human embryonic NSCs. Cerebral hemorrhage rat models were established via injection of autologous arterial blood in caudate nucleus. Two days after modeling, 5 μL BrdU-labeled human embryonic NSCs suspension was transplanted at four points surrounding hematoma cavity in the rats.

通过注射自体动脉血到尾状核制作大鼠脑出血模型,出血后2 d将标有5'-溴脱氧尿嘧啶的人胚神经干细胞悬液移植到血肿腔周围的4 点,1,2周后处死大鼠,相邻脑组织切片行5'-溴脱氧尿嘧啶/微管相关蛋白2和5'-溴脱氧尿嘧啶/胶质纤维酸性蛋白免疫组织化学双染。

24H treatment group compared with 24h model group: the density of light of HSP70 of the group increased and which of TGF-β〓, is not different with the model group; the density of the light the HSP70 of the Baicalin group increased and which of it the TGF-β〓, increased with the model group; the density of light of the Concha Margatitifera Usta is not different and which of TGF-β〓, increased with the model group; the density of light of the Cholic Acid group is not different and the density of light of it of TGF-β〓 increased with the model group; the density of light of the Hefang Group is not different and which of TGF-β〓 is not different than the model group.

合方治疗脑缺血12小时组缺血脑组织TGF-β1光密度值较缺血12小时模型组无显著性差异(p>0.05)。本实验结果发现,与单纯缺血12小时、24小时大鼠相比,药物干预组缺血侧大脑皮层TGF-β1免疫反应明显增强,计算机辅助图像半定量分析两组间缺血侧大脑皮层TGF-β1免疫阳性细胞数差异非常显著。说明药物对局灶性脑缺血损伤可能有一定保护作用,其机制可能与诱导脑细胞TGF-β1合成增加有关。然而,药物调控TGF-β1表达,其促TGF-β1表达增强的机制可能是这样的:脑缺血时TGF-β1mRNA表达增加可能与缺氧诱导神经和胶质细胞刺激生长因子表达有关。

objective to investigate and discuss the application of nerve block anesthesia to the resection of brain gliomaous tumour under sober conversation.

目的 探讨应用神经阻滞麻醉在清醒对话中切除脑胶质瘤的价值与安全性。

Methods Nerve block anesthesia was applied to six patients with brain glioma undergoing craniotomy and resection of glioma under the condition of sober conversation.

目的 探讨应用神经阻滞麻醉在清醒对话中切除脑胶质瘤的价值与安全性。

According to the fluorescence real-time quantitative PCR test, EGFR expression amount was higher in glioma cell line spheroids and glioma stem cellular spheroids, but lower in neural stem cellular spheroids, which was consistent with the results of protein level determination.

荧光实时定量PCR检测显示EGFR mRNA在人脑胶质瘤体外细胞系球体及脑肿瘤干细胞球体中表达含量高,在神经干细胞球体中表达含量极低,与芯片中蛋白水平的检测相一致。

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