神经胶质的

Objective To investigate the response of neurons and astrocytesfrom Sprague Dawley rat brain against tert-buty1 hydroperoxide toxicity,both separately and in cooperation, for the purpose of exploring the interrelation of the two kinds of cells.

目的研究混合培养脑神经元与星形神经胶质细胞对活性氧叔丁基脂氢过氧化物的应激性，以探讨神经元与星形神经胶质细胞的相互作用。

The pink fibers are nerve fibers and the processes of the neuroglia.

的细胞核为各种神经胶质细胞的核，粉红色的纤维为神经纤维及神经胶质细胞突起。

Iron in the form of ferritin is highly toxic and may cause neural death and reactive gliosis. Astrocytes and microglia are responsible for uptake of excessive CSF iron in superficial siderosis.

星形胶质细胞和小胶质细胞可摄取脑脊液中过多的铁，而铁蛋白中铁的毒性又可导致神经元坏死和反应性神经胶质增生。

The brain tissue of the different fetal age rat could be cultured into neural stem cells with the most number of cells for 12.5 dags embryo, which furthermore differentiated into neurons and glial cells.

胎龄为12.5天胎鼠大脑皮质培养出的神经干细胞数量最多，可分化为神经元、神经胶质细胞及少突胶质细胞。

Against glioma in support of the central nervous system of glial cells.

神经胶质瘤侵害支持中枢神经系统的神经胶质细胞。

The malignant growth of neuroepithelial cell will result in glioma with high invasiveness and rapid growth.

神经胶质细胞的恶性生长导致胶质瘤，胶质瘤侵袭性强、生长迅速。

This experiment further investigated the possibility of differentiation of BMSCs in rats into neural stem cells in vitro, and into neural cells and neuroepithelial cells, so as to provide seed cells for cell transplantation of nervous system disease.

目的：已证实骨髓基质细胞可分化为中胚层组织细胞，实验予进一步探讨体外分离培养的骨髓基质细胞向神经干细胞分化的可能性，以及是否能继续定向分化为神经细胞及神经胶质细胞，为神经系统疾病细胞移植治疗提供种子细胞。

Neuroglial cells (oligodendrocytes and astrocytes) play a very important role in establishment and maintenance of normal neuronal functions and formation of neurological diseases.

神经胶质细胞（主要是少突胶质细胞和星形胶质细胞）对于正常神经系统功能的建立和维持及神经系统疾病的产生都起着至关重要的作用。传统观念认为大脑功能的唯一发挥者是神经元。

The St. Jude team first determined that CSCs are located in ascular niches by identifying cells carrying a protein called Nestin that marks stem cells (Nestin+ cells) in four types of brain cancer remoed from patients: medulloblastoma, ependymoma,oligodendroglioma and glioblastoma.

圣犹达研究组研究了病人的四种脑肿瘤：成神经管细胞瘤、室管膜细胞瘤、少突神经胶质瘤和成胶质细胞瘤，通过鉴别名为Nestin的蛋白来识别肿瘤干细胞，第一个验证了肿瘤干细胞存在于血管性的微环境中。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失