神经胶质

The species of extracellular ATP can participate the effect of nerve system not only as the neurotransmitter and modulator but also has a trophic action to glial cell and neuron. It participates in many courses, e.g. cell differentiation and apoptosis, the morphologic change of chondriosome, to stimulate the synthesis and release of cytokine and nerve growth factor. It has a great influence to neurological growth and plasticity.

细胞外ATP类物质不仅可以作为神经递质及调质参与神经系统的功能，还可对胶质细胞及神经元产生营养作用，广泛参与细胞分化及凋亡、细胞线粒体形态变化、刺激细胞因子及神经生长因子的合成及释放等过程，对神经系统的发育及可塑性变化产生重要的影响。

Results GDNF plays extensive roles in the development and disease of motoneuron.

胶质细胞源性神经营养因子(glialcellline-derivedneurotropicfactor，GDNF)最初是从大鼠胶质细胞系B49的条件培养基中分离纯化获得的一个新的神经营养因子，GDNF为分泌性碱性蛋白质，在中枢神经系统和周围器官中有广泛分布，其表达受不同的转录因子等调控。

Results:(1)NSCs form typical neurospheres under adequate concentration in vitro, which are immunoreactive to Vimentin. Typically and terminally differentiated mature neural cells could not be found without the stimulus of mitogen or only under NSCs self-regulation and self-induction;(2)NSCs derived from hippocampus maintain the character of stem cells much longer with better biological behavior; NSCs passed to the 2-3 passage are the best to graft since they have not differentiated;(3)NSCs cultured in vitro could self-regulate and differentiate into neurospheres and progenitors positively immunoreactive to specific antibodies representing neurons, astrocytes, oligodendrocytes and Schwann cells;(4)There are widespread synaptic contacts between various kinds of descendent clones and cells;(5)Neurospheres could be formed without the stimulus of mitogen when NSCs and OECs are cocultured. Many neurospheres and cells immunoreactive to Vimentin, GFAP, MAP2, 02, p75NGFR, GFAP, S-100, Synaptosis, Vimentin, Tau (Tau is only positive in cocultureof HNSCs+HOECs) could be found;(6)The supernatant fluid triturated from adult rat spinal cord stimulates NSCs to differentiate into neurons, but do not terminally differentiate;(7)Fibroblasts and O4 oligodendrocytes are not supported to grow under this culture medium.Part II: Isolation, culture and identification of rat and human olfactory ensheathing cellsOlfactory ensheathing cells/glials are the most powerful cells to enable the regeneration of axons in the central nervous system.

结果表明：①在适宜的浓度体外培养条件下，NSCs能形成典型的神经干细胞克隆球，Vimentin免疫荧光染色阳性，单靠丝裂原刺激或NSCs自我调节和分化诱导，不会产生典型的终末分化的成熟神经细胞；②海马源性的NSCs维持干细胞特性的时间更长，生物学特性更优；③传至第2～3代的NSCs尚未分化时移植最佳；④体外培养的NSCs能自我调控分化为神经元、星形胶质细胞、O2少突胶质细胞、雪旺氏细胞染色阳性克隆球和前体细胞；⑤各种子代克隆球和细胞存在广泛的突触联系；⑥NSCs与OECs联合培养时，不需丝裂原刺激即能形成克隆球，获得大量Vimentin、GFAP、MAP2、O2、p75NGFR、GFAP、S-100、Synaptosis、Vimentin、Tau(Tau只有人HNSCs+HOECs联合培养时出现阳染)染色阳性的克隆球和细胞；⑦脊髓研磨后的上清液刺激神经干细胞向神经元方向分化，但并不出现终末分化；⑧本研究培养条件不利于成纤维细胞、O4生长。

Factor of 1 nerve nutrition and lack gene of nutrition of ill nerve of blood-vessel of head of courage and uprightness is a group of special protein and peptide kind element, this are familial include nerve to grow factor (factor of nutrition of nerve of sex of NGF), head source , nerve - 3, alkalescent grow into fiber cell factor and nerve of sex of colloid cell source.

现将几种重要的非炎性细胞因子和缺血性脑血管病关系的探究进展作以下综述。1神经营养因子和缺血性脑血管病神经营养因子是一组非凡的蛋白质和肽类分子，该家族包括神经生长因子、脑源性神经营养因子、神经营养素-3、碱性成纤维细胞生长因子和胶质细胞源性神经营养因子等。

Objective: To observe the quantitative and morphological changes of astrocytes in nucleus of solitary tract after vagus nerve stimulation.

目的：观察颈部迷走神经千刺激后孤束核内星形胶质细胞数量、形态的改变，同时在电镜水平观察星形胶质细胞与神经元之间突触形态、数量的变化，从胶质细胞的角度阐明VNS治疗神经精神疾病的重要机制。

It has been found that ectogenic GDNF has nervous protective effec,but ectogenic GDNF cannot enter blood brain barrier.

传统观点认为，星形胶质细胞是神经系统的支持细胞，构成神经组织的网架而起支持作用，与神经元的解剖位置关系紧密，参与中枢神经系统的免疫反应，参与构成血脑屏障，目前研究发现，脑缺血时星形胶质细胞异常活跃，通过合成多种蛋白质、细胞因子和神经营养因子等对神经元起保护作用，对缺血性脑损伤的发展和转归具有重要意义。

In order to explore the function of glia in the progress the experiment was performed both in presence of glia and in the absence of glia The mouse mesencephalic cells were exposed with MPP+ with different concentration. Iloprost was used to investigate whether it can prevent dopamine neuron from the neurotoxic of MPP~..

为了观察胶质细胞在其中的作用，将细胞分为未抑制胶质细胞和胶质细胞抑制组；将不同浓度的MPP~+加入体外培养的中脑多巴胺能神经元培养液中，并加入Iloprost来研究其对MPP~+所导致的神经毒作用是否有保护作用。

Results: The findings on CT and MRI were as follows: 57 cases of Oligodendroglioma, 15 cases of Ganglioglioma , 5 cases of Dysembryoplastic neuroepithelial tumor , 3 cases of Pleomorphic xanthoastrocytoma .

结果：少枝胶质瘤47例，间变性少枝胶质瘤10例，节细胞胶质瘤15例，胚胎发育不良性神经上皮瘤5例，多形黄色细胞瘤3例。

In the experimental study section, the rats were divided into four groups, including the normal control, the model, the Piracetam, the QPC, and copied to AD model. After the treatment, we observed the effects on the AD rats' ability of leaning and memorizing, detecting the TChE activity in whole blood and the expression levels of the related biochemical indicators of As includeing GFAP、NGF and BDNF of the AD rats' brain hippocampus, then compared the QPC with other groups to reveal the mechanism of action of the QPC treating AD to bright.

在动物实验部分，我们通过塑造AD动物模型，设立正常对照组、模型组、脑复康组和强脾益智胶囊组，观察强脾益智胶囊对AD模型大鼠空间学习记忆能力的影响，检测AD大鼠全血、海马匀浆液中乙酰胆碱酯酶活性和通过免疫组化的方法检测胶质纤维酸性蛋白、神经生长因子、和脑源性神经营养因子的表达情况来反映星形胶质细胞的增值情况，以期揭示强脾益智胶囊对AD的治疗作用机理。

Human hair keratin ; spinal cord injury ; rat ; tissue engineering ; tissue-engineered spinal cord ; connector ; morphology ; praxiology ; neuron ; axis-cylinder ; nerve fiber ; myelin sheath ; astrocyte ; oligodendrocyte ; microglia ; ependymal cell ; neural stem cells ; macrophage ; multinuclear giant cell ; lysosomal enzyme ; ubiquitin system ; suppression subtractive hybridization ; gene express ; CytohromeoxidaseⅢ; amino acid transporter system A2（Ata2）; Peroxiredoxins 5（prd 5）; cysteine proteinase inhibitor cystatin c

国家自然科学基金；广东省自然科学基金人发角蛋白；脊髓损伤；大鼠；组织工程学；组织工程化脊髓；桥接物；形态学；行为学；神经元；轴突；神经纤维；髓鞘；星形胶质细胞；少突胶质细胞；小胶质细胞；室管膜细胞；神经干细胞；巨噬细胞；多核巨细胞；溶酶体酶；泛素肽酶系；抑制消减杂交；基因表达；细胞色素氧化酶Ⅲ；氨基酸转运蛋白系统A2；抗过氧化物蛋白5；半胱氨酸蛋白酶抑制剂

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷