神经胞质

The lesion, the changes of activity of cholinesterase and acid phosphatase of motor neurons in lateral nucleus of spinal cord anterior horn, the function of axoplasm transportation and nerve conduction, the regeneration of axons and myelin sheath, and the recovery of sciatic nerve function were examined at 7, 14, 30 and 90 days intervals after operation, using Nissl and enzyme histochemistry staining, electrophysiological technique, hydrogen-peroxide oxidoreductase retrograde trace method, axon image analysis, and measurement of sciatic function index. The spinal cord anterior horn of injury side was compared to the correspondence region of spinal cord.

分别于术后7、14、30和90d应用酶组织化学方法、电生理方法、HRP （hydrogen-peroxide oxidoreductase）逆行示踪方法、轴突图象分析方法以及坐骨神经功能指数（sciatic nerve function index，SFI）测量等方法检测坐骨神经损伤后对应脊髓神经元的存活率、神经元胞体酶系变化、损伤神经在轴浆运输、电传导以及轴突、髓鞘再生等方面的恢复情况，探索外周神经损伤后OECs及几丁质对神经元的保护作用以及对新生神经功能恢复的作用，为外周神经损伤的治疗提供新的理论基础。

Methods Eukaryotic expression plasmid pCMVCD was constructed , and identified by re2 striction endoenzyme digestion. CD gene was transfected into NSCs from new2born Wistar rats using Lipofec2tamine2000. Positive clones (named NSCs/ CD cells) were screened by G418 presence. 52Fluorocytosine (52FC) ad2ministration of different concentrations were incubated with NSCs/ CD cells. NSCs/ CD cells viability ratios were mea2 sured by MTT assay.

通过构建真核表达质粒pCMVCD ，限制性内切酶消化鉴定后，采用Lipofectamine 2000 脂质体介导法转染新生大鼠室管膜下区神经干细胞(Neural stemcells ， NSCs)，G418 筛选阳性克隆，加入不同浓度的52氟胞嘧啶(52Flourocytosine ， 52FC)，MTT比色法测定NSCs的生存率。

Concentrations of DA and 5-HT in different regions were measured by high performance liquid chromatography; the expression of SERTmRNA was detected by in situ hybridization; the degeneration of neuron and terminal was demonstrated by Bielschowsky and Glee Marsland silver staining; and the protein expression of GFAP was detected by immunohistochemistry.

2采用高效液相色谱法测定不同脑区神经递质DA和5-HT的含量；采用原位杂交方法检测SERTmRNA；Bielschowsky及Glee Marsland氏改良银浸染法显示神经元胞体、树突和轴突；免疫组织化学方法检测GFAP的表达。

Possible involvement of syntaxin4 and SNAP-23 in exocytosis from human natural killer cells to Porcine Endothelial Cells It was reported that the SNAREs protein complex are involved in many human immune cells and participated in exocytosis of the granules, such as platelet, macrophages and basophilic cells.

SNARE是一种参与神经递质释放的胞内蛋白质，在介导分泌性囊泡运动和融合中发挥重要作用，也参与某些免疫相关细胞的囊泡运动、效应物质分泌和免疫效应调节等。

N IND sections, the picture of dysplasia neurons can be distinguished from the normal easily with a smaller cell and cytoblast diameter, a distinct increase of number, less and stain thickened cytoplasm, nucleolus exit or not.

ND病变肠段粘膜下及肌间均有巨神经丛，丛内各成分细胞均呈阳性反应，神经丛内有大量大小不等细胞，为胶质细胞与发育不良神经元，其中可见部分细胞胞体较大。

Meanwhile, the gap junctional connexin was regulated by some growth factors, neurotransmitters,hormone.

同时，胞间连接蛋白又受着许多生长因子、神经递质、激素等的调控。

PRL-LIR positive perikarya were mainly seen in nucleus suprachiasmaticus, nucleus paraventricularis preopticus, nucleus supraopticus, nucleus hypothalamus paraventricularis, nucleus periventricularis arcuatus and nucleus accumbens. PRL-LIR fibers and their terminals were detected in paleostriatum and median eminence.

结果，PRL阳性胞体主要分布在视交叉上核、视前室旁核、视上核、下丘脑室旁核、弓状核和伏隔核，旧纹状体、正中隆起存在大量阳性纤维末梢，在侧脑室腹侧的室管膜和脑基底神经胶质板上也存在PRL阳性神经元。

In electron microscopy endothelial cell of capillary and mitochondrial was edematous obviously, the basement membranes were thickened. RGCs were vacuolated, chromatin of nucleus aggregated marginally decreased and densification. Some of RGCs were necrosis, nucleus were dissolved, mitochondria was edematous, the rough endoplasmic reticula decreased obviously.

电镜下视网膜毛细血管内皮细胞明显肿胀，胞体变圆，突向管腔，线粒体肿胀，空泡化，基底膜增厚，视网膜神经节细胞胞体变形，核明显固缩，核膜消失，胞浆空泡化，线粒体肿胀，细胞表面突起减少，多聚核糖体及粗面内质网显著减少。

RESULTS: In the verapamil group, the fibroblast were round or oval, and there were tiny cell process, less alpha substance, atrophic and decreased rough endoplasmic reticulum in kytoplasm and abundant vesica. In control group, there were fusiform or polygon fibroblast, and much matrix among cells, abundant rough endoplasmic reticulum and ribosome in cytoplasm and less vesica were found.

结果：维拉帕米组神经瘢痕成纤维细胞呈球形或椭圆形，细胞突起细小，胞间网状物质少，胞浆中粗面内质网萎缩且数量减少，囊泡丰富；对照组成纤维细胞呈梭形或多角形，细胞间基质较多，细胞浆内粗面内质网及核糖体丰富，囊泡少见。

The ultrastructural characteristics of the lateral horn revealed bypresent study included:(1) A little amount neuropil existed between cell bodies in the cell clusters.

侧角区超微结构观察中见到的特点是：(1)侧角细胞簇内神经元胞体之间只有少量神经毡，有时出现相邻胞体的质膜邻接。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。