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Expanded MSCs were induced to differentiate into nerve cells withthree different treatment protocol in the prensent study . On the following day, the medium was replaced with preinduction medium consisting of DMEM, 20% FCS, and 10 ng/ml basic fibroblast growth factor. After 24 h, the preinduction medium was removed, the cells were washed twice with PBS, and neuronal induction medium containing DMEM supplemented with 2% DMSO and200 m bu-tylated hydroxyanisole was added. In later experiments, we used DMEM with 5 mM p - mercaptoethanol or sulfo - glycerine as an alternative neuronal induction medium for the same incubation.

将待诱导分化的MSCs按0.4x10~6/ml接种于事先放置有消毒盖玻片的六孔板内制备细胞爬片,分别采用三种不同的方法诱导第2代至10代的MSCs向神经细胞分化,即方法1先用含bFGF的培养基进行预诱导,之后再用含叔丁基对羟基茴香醚,和二甲亚砜的无血清培养基进行诱导;方法2和方法3用B-巯基乙醇和硫代甘油进行预诱导,然后不同浓度的上述物质进行正式诱导。

GnRH somata were found in the dorsal midbrain, preoptic area and the periventricular rostral midbrain.

GnRH神经细胞本体主要分布在前脑、视前区、下视丘及中脑后部靠近脑室的位置。

DATA SYNTHESIS: Many studies revealed that pharmacological elements of herbs had protectively treatment effects on harmed neural cells.

资料综合:多项观察结果显示,部分中草药的药学成分对神经细胞的损伤有治疗及保护作用。

A branched protoplasmic extension of a nerve cell that conducts impulses from adjacent cells inward toward the cell body.

树突神经细胞中一种树枝状细胞质突起,它把邻近细胞的刺激传到本细胞。

Methods The skeletal muscle specimens with obviously regenerating muscle fibers were obtained from 9 patients with pseudohypertrophic muscular dystrophy and 5 patients with polymyositis. Then frozen serial sections were made and the sections were treated with HE stain, immunohistochemistry stains including antiEzrin and antiNeural cell adhesion molecule monoclonal antibodies. The pathological changes and the expression of Ezrin were observed.

取肌纤维再生活跃的假肥大型肌营养不良(DMD,9例)和多发性肌炎(PM,5例)患者的骨骼肌标本,冰冻连续切片,进行HE染色及抗Ezrin、抗神经细胞黏附分子单克隆抗体免疫组化染色,观察被检肌的病理改变和Ezrin蛋白的表达。

Other experiments revealed slight variations in the size, number, arrangement, and interconnection of the nerve cells, but as far as psychoneural correlations were concerned, the obvious similarities of these sensory fields to each other seemed much more remarkable than any of the minute differences.

其他实验显示,即使在规模、数量、安排和神经细胞的连接上做微量的调整,就心理神经的关联性而言,这些感觉领域的明显的相似之处,似乎远远超过了任何明显的细微差别。

Other experiments revealed slight variations in the size,number,arrangement,and interconnection of the nerve cells,but as far as psychoneural correlations were concerned,the obvious similarities of these sensory fields to each other seemed much more remarkable than any of the minute differences.

尽管其他实验显示在神经细胞的大小、数量、排列和相互连接上有一些小的差异,但是就心理-神经的关系而言,这些感官区域彼此之间的明显的相似性看起来比起微小的差异更为令人注目。

Other experiments revealed slight variationsin the size,number, arrangement,and interconnection of the nerve cells,but as far as psychoneural correlations were concerned,the obvious similarities of these sensoryfields to each other seemed much more remarkablethan any of the minute differences.

尽管其他实验显示在神经细胞的大小、数量、排列和相互连接上有一些小的差异,但是就心理-神经的关系而言,这些感官区域彼此之间的明显的相似性看起来比起微小的差异更为令人注目。

Other experiments revealed slight variations in the size,number, arrangement, and interconnection of the nerve cells,but as far as psychoneural correlations were concerned,the obvious similarities of these sensory fields to each other seemed much more remarkable than any of the minute differences.

第 37 页(共 101 页)#尽管其他实验显示微小的差异/在大小,数量,排列方式和相互连接上/神经细胞的/,但是就心理与神经的相互关系而言/,这些明显的相似性感官区域的/彼此间的/看起来重要的多/比任何微小的差异。

Although Other experiments revealed slight variations (in the size, number, arrangement, and interconnection of the nerve cells), but/as far as psychoneural correlations were concerned/, the obvious similarities (of these sensory fields to each other) seemed much more remarkable/than any of the minute differences/.

尽管其他实验显示(在神经细胞的大小、数量、排列和相互连接上)有一些小的差异,但是/就心理——神经的关系而言/,明显的相似性看起来远远要/比其微小的差异/更为令人注目。

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