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To study the effect of IL-1 on the expression of APP gene in neuronal cell, 80% confluent cultures of human neuroblastoma SH-SY5Y cells were treated with IL-1β.

为研究IL-1对神经细胞中APP基因表达的影响作用,用IL-1β刺激培养至80%融合的人神经母细胞瘤SH-SY5Y细胞。

DGCs in girdin-deficient neonatal mice exhibit deficits in axonal sprouting in the cornu ammonis 3 region of the hippocampus.

对新生小鼠齿状回缺失Girdin蛋白的细胞的研究表明,小鼠神经细胞不能形成轴突。

At the same time, they recorded the field potential of thousands of nere cells in the cerebral cortex.

同时,他们也记录了大脑皮层数千个神经细胞的场位。

MAIN OUTCOME MEASURES: Structure changes in cerebral cortical neural cells in rats of each group.

主要观察指标:各组大鼠大脑皮质神经细胞的结构变化。

Objective:To study the apoptosis and the mechanism in rat cortical neural cells induced by high iodine.

目的:观察长期高碘对大鼠皮层神经细胞凋亡的影响,并探讨其作用机制。

Objective To investigate the effect of Shenmai injection on hypoxic injured cortical neurons in cultured rats.

目的 探讨参麦注射液对培养鼠大脑皮层神经细胞缺氧损伤的保护作用。

The method of primary hippocampal nerve cell culture was used to observe the effect of CRF and corticosterone.

连续加药9天,不同浓度的CRF对离体海马神经细胞的存活率没有明显的影响。

Learning and memory performance of rats was measured by using Morris water maze first and Y-maze afterwards. Neurons in the dentate gyrus, CA3 and CA1 regions of the hippocampus were stained by using Cresyl violet method and counted.

采用Morris 水迷宫和Y-迷宫测试大鼠学习与记忆成绩,并用Cresyl violet染色法对大鼠海马结构进行神经细胞计数。

Hypoxia induces dedifferentiation of neuroblastoma cells in vivo and in vitro, and under oxygen-compromised conditions the Notch cascade is activated.

在体内和体外缺氧可诱生成神经细胞瘤的去分化,而氧妥协的情况下,Notch信号则被激活。

Methods: We divide the rats into 5 groups in random, with Morris method, detect the change of some active oxygen radicals such as super-oxide demitasse, Nitric Oxide Synthase, malondialdehyde etc. Also, we detect the change of cholinesterase, Monoamine Oxidize; using HE,PAS staining, immuno-histochemical technique, detect hippocampus CA1 cerebral pathology slices optical density value of NOS, CHE and lipofuscin of neural cell; detecting apoptosis rate and the expression of P53 , which are neuronal apoptosis related genes.

大鼠随机分为5组,采用"Morris水迷宫"法观察脑缺血性模型大鼠学习记忆功能,检测超氧化物歧化酶、一氧化氮合酶、丙二醛等自由基代谢异常的改变,检测胆碱酯酶、单胺氧化酶等老化相关酶的变化、脑组织神经细胞免疫组织化学技术、检查海马CA1区脑组织的病理切片,检测NOS、CHE光密度值;流式细胞技术:检测细胞凋亡率,及促细胞凋亡基因、P_(53)蛋白表达;病理切片采用HE染色、PAS染色及免疫组化法检查,检测对大鼠海马神经元细胞一般病理变化及脑细胞内脂褐含量。

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