神经组织学

The protein and phospholipid constituent in membrane of neurocyte in brain of chicken with encephalomalacia was separated and studied with the method of SDS-PAGE electrophoresis and the method of high performance-thin layer chromatography respectively The fluidity of neurocyte membrane of chicken with encephalomalacia was determined for the first time The content of Ca and trace elements were determined in chicken encephalomalacia systematically and the status of Fe ion in cerebellum was observed. The activity of enzymes that were relative to metabolism of energy and neuro-medium in brain were checked systematically with the method of enzyme-histochemistry. Pathological examination was carried out by microscopic and ultramicroscopic observation. The apoptosis of neurocyte in brain was checked.

在进行临床观察、定期剖杀并检测体内V〓含量、体重和脑重的基础上，重点研究了与神经损伤有关的以下几个方面的内容：系统地检测了体内自由基代谢和抗氧化系统功能的变化；首次采用SDS-PAGE电泳法分离并检测了雏鸡脑软化症神经细胞膜蛋白组分，并以高效薄层层析法检测神经细胞膜磷脂组分；测定了脑软化症雏鸡神经细胞膜的膜流动性；首次系统检测了脑软化症雏鸡体内钙及微量元素含量，并进行了小脑组织总铁离子状态观察；采用酶组织化学方法系统地观察了脑组织中与脑能量代谢和神经递质有关的酶的活性；对相关组织进行了病理组织学和超微结构观察；并对脑组织中神经细胞的凋亡进行了检测。

The results showed that LRK could significantly inhibit the mammary hyperplasia caused by estrin, reduce the content of serum 〓, raise P level, promote the production of DA in hypothalamus and mammary tissue, decrease the release of 5-HT, inhibit the secretion of serum PRL, reduce the sensitivity of mammary tissue to estrin and progestogen, prevent the expression of mammary tissue on PCNA, the effect was equal to triphenylamine oxide, obviously superior to the control group of Ru Zeng Ning.

通过实验研究，建立了具有肾虚肝郁证侯群的乳腺增生病理模型，利用免疫组化技术、图像分析系统、荧光分光光度法、放射免疫法等现代医学研究方法，从病理形态学、医用体视学、神经内分泌学、阴道细胞学等方面，观察了利乳康口服液对模型动物整体状态、乳腺组织病理形态、血清激素、神经递质以及卵巢功能的影响，结果表明利乳康口服液能明显抑制雌激素引起的乳腺组织增生，降低血清E〓含量，升高P水平，促进下丘脑及乳腺组织中DA的产生，减少5-HT释放，抑制血清PRL分泌，降低乳腺组织对雌、孕激素的敏感性，阻止乳腺组织PCNA的表达，其效应与三苯氧胺相当，明显优于乳增宁对照组；另外，利乳康口服液还能缓解模型动物肾虚肝郁证侯群，增加其体重，调节其整体状况，改善模型动物不规则动情周期，减轻其子宫及卵巢的病变，抑制子宫内膜的过度增长，调节卵巢功能，揭示了中药利乳康口服液对神经内分泌和卵巢功能的整体调控作用，以及对本病发生、发展的预防作用

There was no significant difference in number of myelinated nerve fiber, thickness of myelin sheath, and area of nerve tissue between experimental and autoallergic neural transplantation group.

实验结果验证了无细胞神经移植物复合骨髓间充质干细胞构建组织工程人工神经修复大鼠坐骨神经缺损，可以促进神经组织学的修复重建和功能的恢复。

To study the ossification effect by histology research on tissue engineered bone by transplanting nerval bundles of different types.

通过观察不同神经束植入组织工程骨后组织学的变化，探讨组织工程骨神经化构建的效果。

The function of oculomotor nerve was observed periodically, and neuroanatomical and histological aspects were observed with light microscope and electron microscope.

术后观察动眼神经功能并进行神经组织学和解剖学研究。

Then transplantation of the sciatic nerve from donor rats to the recipient rats in two groups were progressed.7 days after nerve transplantation,the contents of cd4 and cd8 in peripheral plasma of recipient rats were detected by flow cytometry.8 weeks after nerve transplantation,transplanted nerve were observed under light microscope.results:compared with nonirradiated group,the percentage of cd4 and the ratio of cd4 to cd8 in uvb irradiated group were significantly decreased（p＜0.05,p＜0.01）,cd8 percentage was significantly increased（p＜0.01）.conclusion:uvb-irradiated donor splenocytes were injected in the recipient rats through caudal veil,which could induce immunological tolerance to allogeneic transplantation.

未照射组注射未经紫外线照射的供体脾细胞，照射组注射经紫外线照射的脾细胞，7 d后移植供体神经。术后7 d行外周血t淋巴细胞亚群检查，术后8 w行移植神经组织学检查。结果：照射组cd4+百分率、cd4+/cd8+比值较非照射组明显降低（p.01，p.05），cd8+百分率较非照射组明显升高（p.01）。组织学检查显示，照射组的再生神经较非照射组多。结论：尾静脉内注射经紫外线照射的异基因脾细胞可诱导对异体神经移植的特异性免疫耐受。紫外线；脾细胞；免疫耐受；坐骨神经

Method］twenty six wistar rats were divided into two groups, with 13 in each group, peroneal nerve was transacted on the right side of all the animals, in one group, the proximal end of peroneal nerve was turned back and ligated, the distal end was sutured to the side of tibial nerve with perineurial window, in another group,both ends of the transacted peroneal nerve were turned back and ligated as contrast, no operation was done on the left side.

方法］wistar大白鼠26只，随机分实验组和对照组，每组13只，实验组右侧腓总神经切断后，近端反转结扎，远端与胫神经外膜开窗处行端侧吻合，对照组右侧腓总神经切断后，两端均反转结扎，两组动物左侧作正常对照，不作处理。术后3个月，行双侧腓总神经、胫前肌组织学检查，胫前肌湿重测定，运动终板检查。

The adipose derived stem cells of inbreds train of rat wereinduced to differentiate into schwann-like cells and the cells were injectedinto the acellular nerve allograff.The proliferation or adhersion of these cells on the graft were observed by inverted microscope or ScanningElectronic Microscope. Activity of cells were detected using MTT. 10mmnerve gap of inbreds train of rats in two group were bridged by tissue-engineered peripheral nerve or autogenous nerve, the effect wereappraised by naked eye, recovery rate of sciatic function index,nerve-electrophysiological, histology, Transmission ElectronMicroscopy and quantitative analysis of recovery rate of myelinated fiberpopulations,diameter of myelinated fiber and thickness of myelin sheat.

按照前述方法培养并诱导近交系大鼠脂肪干细胞向类Sehwann分化，将诱导分化的脂肪干细胞悬液注入已制备的去细胞同种异体神经支架管中，倒置显微镜观察细胞生长情况；扫描电镜下观察细胞在材料上附着情况；四唑盐比色试验测定细胞活性；取两组近交系大鼠，分别用组织工程化神经和自体神经桥接10mm神经缺损，通过大体观察、坐骨神经功能指数恢复率的测定、神经电生理的测定、组织学光镜观察、透射电镜观察、再生有髓神经纤维计数恢复率、神经纤维直径恢复率、髓鞘厚度恢复率的测定等指标评价实验效果。

Methods:An em bedding audio-frequency electric stimulator was used in the rabbit's sciatic nerve defect bridged with auto genous FTMG.The graft appearance and vascular growth of its surface were observe d at 4,8 and 12 weeks post-operation respectively.

在自体冻融骨骼肌移植体桥接兔坐骨神经1.0 cm缺损时应用全置入式音频电刺激器，分别在4，8，12周观察移植体外形及表面血管情况，神经组织学，神经电生理，神经免疫组织化学和透射电镜等指标。

The rate of enteric transit was measured, and the histological changes in nitriergic nerves in intestinal myenteric plexus of scalded rat were observed as well. The densities of neurons and ganglions with positive nitric oxide synthetase as well as ICC in duodenal myenteric plexus were assessed for both groups.

用酶组织化学方法观察回肠肌间神经丛内氮能神经的组织学改变，并对两组大鼠回肠肌间神经丛内一氧化氮合酶阳性神经元、神经节和Cajal间质细胞的密度进行测定。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了