神经组织

METHODS:Nestin expression was detected in 33 cases of neuroepithelial tumors using the immunocytochemical staining. The Nestin positive and negative cells were isolated by magnetic activated cell sorting. Nestin positive cells were culutered, identified, and induced to differentiate. The expression of Nestin protein was detected in Nestin+ cells by RTPCR.

采用免疫组化法检测神经上皮肿瘤组织中Nestin的表达情况，免疫磁珠法分选肿瘤组织中的Nestin+细胞，悬浮细胞培养法进行Nestin+细胞的增殖、分化与鉴定，RTPCR法检测Nestin蛋白在其中的活性表达情况。

The reasons are probably that the micro-environment of cultivation is not perfect enough, engineered bone can not achieve the synchronization of vascularization and neurotization, and construction of the complex of tissue-engineered bone and periosteum is still lack of research.

可能的原因是细胞的培养微环境不够完善，没有实现组织工程骨的同步血管化和神经化，组织工程骨与骨膜复合体的构建目前仍缺乏研究。

The reasons are probably that the micro-environment of cultivation is not perfect enough, engineered bone can not achieve the synchronization of vascularization and neurotization, and construction of the complex of tissue-engineered bone and periosteum is still lack of research.

可能的原因是细胞的培养微环境不够完善，没有实现组织工程骨的同步血管化和神经化，组织工程骨与骨膜复合体的构建目前仍缺乏研究。仁和代写代发医学论文：提供专业的医学论文写作指导，修改，翻译，发表服务。

Of the index finger back side island flap, the seaming finger nerve back lateral finger artery reversiveisland flap, the neighbour finger skin flap, the dorsal metacarpal artery reversive island flap and theposterior interosseous artery reversive forearm flap, groin skin flap,ilium skin flap and thoracic -umbilical flap were 197 cases.

杜昭，蔡文，黄德征（广西医科大学第六附属医院手外科，广西玉林537000）摘要：目的探讨手部创伤组织缺损显微外科修复方法及其效果。方法采用食指背侧岛状皮瓣、缝接指神经的背侧支指动脉逆行岛状皮瓣、邻指皮瓣、掌背动脉逆行岛状皮瓣和前臂骨间后动脉逆行皮瓣、髂腹股沟皮瓣、髂骨皮瓣及胸脐皮瓣转位修复手部创伤组织缺损197 例。

Four aspects of research achievements include:(1)several imaging correction methods and strained estimation methods for biological tissues elasticity imaging were investigated as well as elasticity reconstruction and phantom, in vitro and in vivo elasticity imaging experiments;(2)in the fields of biological microsystem and perfusion evaluation for microvascular system, three kinds of microbubbles whose shell are albumin, sugar ester and surfactant respectively were designed and produced, then the methods to estimate the thickness of their shells were presented, moreover, the interacting mechanism of ultrasound contrast agents and tissues, as well as protein drug delivery under the condition of ultrasound sonification were studied in the molecular level;(3) At the aspect of high intensity focused ultrasound therapy system, we designed and constructed the 256-element HIFU transducers using 1-3 composite materials. And furthermore we developed the integrated HIFU system with 256-element HIFU transducers. And we made preliminary and important progresses in the research of HIFU bioeffect, lesion estimation, techniques of monitoring and imaging and characterizing of structure of HIFU transducer;(4)The real-time method of voice source regeneration based on compensative regulation of physiological parameters was investigated in the field of medical rehabilitation. We addressed the physiology and biophysics of vocalization, modeled voice source regeneration based on compensative regulation mechanism, and put forward an integrated evaluation about the speech rehabilitation and the regenerated voice source; Sixty-one archived journal papers were published, as well as ten in international journals, and eighteen publications were embodied by SCI.

成果包括：（1）在生物组织弹性成像方面，研究了多种图像校正方法和应变估计方法，进行了弹性重构和仿体、离体及在体超声弹性成像实验研究；（2）在生物微系统和微血管血流灌注成像方面，制备了三种不同包膜超声造影微泡，研究了纳米包膜厚度检测与估计方法以及不同纳米包膜造影微泡断裂与药物释放机制，并在分子层次上开展了超声与生物组织相互作用机制以及蛋白质药物超声乳化微囊包裹的研究；（3）在阵列式高强聚焦超声治疗与监控成像技术方面，设计制作了新的HIFU换能器，解决了256阵元单焦点和多焦点合成HIFU治疗系统关键技术问题，并在HIFU生物效应与创伤性评价方法、HIFU过程监控成像技术和薄层复合结构与HIFU 换能器界面特性评价等方面的研究取得了初步而重要的进展，具有重要应用前景；（4）在语音听觉系统特别是嗓音康复方面，研究了基于发声系统补偿调节机制的实时多模式嗓音源再生方法和发声生理，建立基于神经、肌肉和空气动力系统的综合补偿调节模型，提出了相应的评价系统。

Four aspects of research achievements include:(1)several imaging correction methods and strained estimation methods for biological tissues elasticity imaging were investigated as well as elasticity reconstruction and phantom, in vitro and in vivo elasticity imaging experiments;(2)in the fields of biological microsystem and perfusion evaluation for microvascular system, three kinds of microbubbles whose shell are albumin, sugar ester and surfactant respectively were designed and produced, then the methods to estimate the thickness of their shells were presented, moreover, the interacting mechanism of ultrasound contrast agents and tissues, as well as protein drug delivery under the condition of ultrasound sonification were studied in the molecular level;(3) At the aspect of high intensity focused ultrasound therapy system, we designed and constructed the 256-element HIFU transducers using 1-3 composite materials. And furthermore we developed the integrated HIFU system with 256-element HIFU transducers. And we made preliminary and important progresses in the research of HIFU bioeffect, lesion estimation, techniques of monitoring and imaging and characterizing of structure of HIFU transducer;(4)The real-time method of voice source regeneration based on compensative regulation of physiological parameters was investigated in the field of medical rehabilitation. We addressed the physiology and biophysics of vocalization, modeled voice source regeneration based on compensative regulation mechanism, and put forward an integrated evaluation about the speech rehabilitation and the regenerated voice source; Sixty-one archived journal papers were published, as well as ten in international journals, and eighteen publications were embodied by SCI.

中文摘要：成果包括：（1）在生物组织弹性成像方面，研究了多种图像校正方法和应变估计方法，进行了弹性重构和仿体、离体及在体超声弹性成像实验研究；（2）在生物微系统和微血管血流灌注成像方面，制备了三种不同包膜超声造影微泡，研究了纳米包膜厚度检测与估计方法以及不同纳米包膜造影微泡断裂与药物释放机制，并在分子层次上开展了超声与生物组织相互作用机制以及蛋白质药物超声乳化微囊包裹的研究；（3）在阵列式高强聚焦超声治疗与监控成像技术方面，设计制作了新的HIFU换能器，解决了256阵元单焦点和多焦点合成HIFU治疗系统关键技术问题，并在HIFU生物效应与创伤性评价方法、HIFU过程监控成像技术和薄层复合结构与HIFU 换能器界面特性评价等方面的研究取得了初步而重要的进展，具有重要应用前景；（4）在语音听觉系统特别是嗓音康复方面，研究了基于发声系统补偿调节机制的实时多模式嗓音源再生方法和发声生理，建立基于神经、肌肉和空气动力系统的综合补偿调节模型，提出了相应的评价系统。

The findings of the newly identified goose paramyxovirus strain GPMV/HBexperimental infection were as follows: The challenge caused high morbidity and highmortality.The clinical signs included severe depression, decreased appetite or anorexia,white,yellow and green diarrhea, with neural symptoms;pathological changes showed thathaemorrhage and ulceration of intestine mucosa, necrosis of spleen and pancreas,andedema of liver and kidney, uratic deposition in kidney.Eosinophilic inclusion bodies werefound in the cytoplasm of pancreas and a large quantities of fiber protein were found inintestine. Lots of hemosidefin was found in some area of liver and kidney. A number ofT、B lymphocytes reduced sharply in spleen,lymph nodes in dead geese. Transmissionalelectron microscope showed that some cells had the morphological characteristics ofmitochondria swelling, morphological changes were condensation of nuclear chromatininto dense masses,followed by its marginating against the nuclear envelope, andespecially concentration of cytoplasm association with lots of lipid droplet.

人工感染雏鹅实验中，雏鹅临床表现为高发病率和高死亡率，食欲和饮水减少，拉白色、黄绿色稀粪，伴有神经症状；常规组织学观察，脾脏和胰腺肿大，有白色坏死斑点，肠道出血和坏死，肝脏肿大变性，肾脏肿大变性有尿酸盐沉着等；用特殊染色和组织化学方法首次对人工感染鹅副粘病毒的雏鹅的重要脏器进行研究，结果表明：胰腺中发现病毒包涵体，肠道中大量纤维素以及胶原纤维增生，肾脏、肺脏大量含铁血黄素沉着，免疫器官如脾脏和淋巴结中T、B淋巴细胞大量减少；电镜下多种细胞呈现线粒体水肿，多种实质细胞的染色质固缩，染色质边集，特别是细胞内容物如脂肪滴增多的病理变化特征。

The latency of the waves of SEP or MEP is extracted. But it still is the best in all. The control group is the worst of all. The wave can be found again after the spinal cords are hemisectcd in control group. 5.. Seen from the immunohistochemistry of NF, Schwann cells can promote neural dendrite outgrowth toward the area of hemisected spinal cord.

神经纤维的兔疫组化染色脊髓半切损伤后，经NF的免疫组织化学技术染色显示：移植雪旺细胞可以促进神经纤维向脊髓损伤区域生长，抗体封闭 LI后神经纤维的生长受到抑制，再生神经纤维数相对减少，这从免疫组织化学方面说明口对神经再生具有影响。

The comparison and analysis of relevant results.●Application for financial data analysis and prediction. The research work is summarized as follows At first, we have proposed a reformulating and revolutionizing concept for neural networks information processing and transformation mechanism based on the coupling between field organization transformation and field action transformation.

场组织变换通过神经网络的隐单元，把输入模式转换为相关的结构表示，抽取输入模式的主要结构特征，形成外部环境在反应变换中的结构表示；场反应变换把由场组织变换形成的特征表示，转换为实际期望的输出。

In this thesis, the phase diagram of Zn-Al binary system on 10~69.5 w% was reinvestigated by using differential scanning calorimeter, X-ray diffraction and pushrod dilatometer. The process of isothermal transformation during the aging of high-Aluminum-Zinc-based alloy was studied by using differential scanning calorimetry, and the aging behavior of the alloy was analyzed at the aspect of thermal analysis kinetics. The change of microstructure which the alloy was being heated was observed on high-temperature metallurgical microscope. The microstructure and wear-friction properties were compared among the alloys treated with several different heat treatment processes, and a model for heat treatment processes was proposed based on artificial neural network,so as to predict the relationship between friction coefficient and parameters of aging treatment such as aging temperature and time.

本文采用高温X射线衍射、示差扫描量热分析、热膨胀等方法重新测定含锌量范围为10％～69.5％的Zn-Al系二元相图，利用示差扫描量热分析法，研究高铝锌基合金时效等温转变过程，并从热分析动力学的角度分析高铝锌基合金合金时效行为；使用高温金相显微镜观察合金加热时的组织变化；比较几种不同热处理工艺对高铝锌基合金显微组织与耐磨性能的影响并利用人工神经网络建立高铝锌基合金热处理工艺模型，预测合金摩擦系数随时效温度及时间变化规律。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。