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For those in control group (n=14), the animals did not undergo any surgery of the nerve fibers and ganglia. In Vi group (n=9), the animals underwent bilateral resection of the nasociliary and postganglionic parasympathetic nerve fibbers. In SCG group (n=8), the animals underwent bilateral resection of supper cervical ganglia. In V1+SCG group (n=9), the animals underwent both surgeries as Vi and SCG group did. In sham operation group (n=11), the animals underwent carotid manipulation with blunt-tipped forceps as well as dissection of nasociliary and postganglionic parasympathetic nerve fibers around the ethmoidal foramen, without cutting any nerves. SCS was performed on rats and CBF was monitored by laser Doppler flowmeter recordings.

SD大鼠分5组:对照组(n=14,不对神经纤维和神经节作任何手术处理)、V1组(n=9,切断双侧鼻睫状神经及其副交感节后纤维)、SCG组(n=8,切断双侧颈上神经节)、V1+SCG组(n=9,切断双侧鼻睫状神经及其副交感节后纤维和双侧颈上神经节)、假手术组(n=11,手术暴露双侧鼻睫状神经及其副交感节后纤维和双侧颈上神经节,但不切断神经纤维或神经节);给予大鼠SCS,并用激光多普勒实时记录脑血流。

Control group, the animal underwent SCS and LDF recordings without any surgery of the nerve fibers and ganglia. V1 group, the animal underwent bilateral resection of the nasociliary and post-ganglionic parasympathetic nerve fibbers. SCG group, the animal underwent bilateral resection of supper cervical ganglion. V1 + SCG group, the animal underwent both surgeries as V1- and SCG-group animals did. Sham group, the animal underwent the carotid manipulation with blunt-tipped forceps as well as the dissection of nasociliary and post-ganglionic parasympathetic nerve fibers around the ethmoidal foramen, but without cutting any nerves.

动物分成5组,对照组:不对神经纤维和神经节作任何手术处理;V1组:切断双侧鼻睫状神经及其副交感节后纤维;SCS组:切断双侧颈上神经节;V1+SCG:切断双侧鼻睫状神经及其副交感节后纤维及双侧颈上神经节;假手术组:手术暴露双侧鼻睫状神经和其副交感节后纤维及双侧颈上神经节,但不切断神经纤维或神经节。

Results (1) In the controls and the dilated intestine segment of the HD,CAD-positive ganglionic cells were observed in myenteric and submucosal plexus.

结果 (1)在正常对照组与HD扩张段组肠壁肌间和黏膜下层可见CAD只对神经丛中神经节细胞阳性表达,对神经纤维与神经胶质细胞均不表达。S-100染色与CAD染色相反,神经纤维与神经胶质细胞均阳性表达,神经节细胞阴性表达,表现为阳性神经丛中细胞状&空白区&。

That membrane proceeds to wrap over the nerve fiber with machinelike precision, spiraling down to create the distinctive sausage shape, tightening itself over the fiber like a threaded nut.

这层隔膜就象机器操作那样的精确包裹在神经纤维上,并旋转缠绕成特殊的香肠形状。就象螺母那样紧紧地包在神经纤维上。

Myelinated nerve fiber count was 44.5% as compared to normal control group.

端侧组肌皮神经有髓神经纤维计数占正常组的44.5%,有髓神经纤维髓鞘厚度、最大直径、最小直径分别占正常组的85.9%、77.3%和65.5%。

In normal stomach wall, on the other hand, the SP-IR positive fibers were only found in myenteric and submucous layers, some fibers from the myenteric plexus can also be seen tending to the mucosa.

结论胃癌组织内有神经纤维的存在,SP阳性纤维分布于胃癌细胞间,提示这些神经纤维的存在可能对胃癌细胞的某些生物学特性起调控作用。

The human bile duct carcinoma cell line QBC_(939) was neurotropic. The co-culture model of DRG/nerve cell and carcinoma cells could simulate the process of the tumor cell going through the matrix and invading the nerve fiber.

结论DRG/神经细胞-肿瘤细胞共培养模型较好的模拟了肿瘤细胞穿过细胞基质,浸润神经纤维的过程,人胆管癌细胞QBC939细胞对神经纤维具有亲嗜性。

Results A few stained neurons were observed at the inferior salivatory nucleus and dorsal nucleus of vagus nerve and nerve fibers at double sides, while the superior salivatory nucleus was not marked. The labeled neurons and fibers at the both side were found in the facial nucleus, nucleus ambiguous and motor trigeminal nucleus.

结果 在一般内脏运动核、双侧迷走神经背核、下泌涎核均可看到少量逆行标记细胞及神经纤维末梢,但上泌涎核未发现任何标记,在特殊内脏运动核的面神经核、疑核、三叉神经运动核团双侧均见逆行标记细胞及神经纤维终支。

Result: Schwann-like cells disperse in the hole of nerve graft at oneweek under Scanning Electronic Microscope, the processes of thesatellite-like cells adhere to the wall of graft.There was no statisticaldifference of activity of cells in both group(P>0.05) using MTT. At 12weeks,the result of appearance, histology and TEM were similar, therewere no statistical difference in SFI%, NEP and quantitative analysis ofrecovery rate of myelinated fiber populations, diameter of myelinatedfiber and thickness of myelin sheat.

结果:1w后扫描电镜下可见接种的类Schwann细胞分散在材料表面的孔隙内,细胞形态为星状,细胞突起与支架管壁粘附;MTT法测定细胞活性示神经支架组在各时间点与对照组无显著性差异P>0.05;12w时两组动物坐骨神经外观、组织学光镜观察、透射电镜观察结果相似、两组坐骨神经功能指数恢复率、神经电生理、再生有髓神经纤维计数恢复率、神经纤维直径恢复率、髓鞘厚度恢复率等指标相比无显著性差异(P>0.05)。

The adipose derived stem cells of inbreds train of rat wereinduced to differentiate into schwann-like cells and the cells were injectedinto the acellular nerve allograff.The proliferation or adhersion of these cells on the graft were observed by inverted microscope or ScanningElectronic Microscope. Activity of cells were detected using MTT. 10mmnerve gap of inbreds train of rats in two group were bridged by tissue-engineered peripheral nerve or autogenous nerve, the effect wereappraised by naked eye, recovery rate of sciatic function index,nerve-electrophysiological, histology, Transmission ElectronMicroscopy and quantitative analysis of recovery rate of myelinated fiberpopulations,diameter of myelinated fiber and thickness of myelin sheat.

按照前述方法培养并诱导近交系大鼠脂肪干细胞向类Sehwann分化,将诱导分化的脂肪干细胞悬液注入已制备的去细胞同种异体神经支架管中,倒置显微镜观察细胞生长情况;扫描电镜下观察细胞在材料上附着情况;四唑盐比色试验测定细胞活性;取两组近交系大鼠,分别用组织工程化神经和自体神经桥接10mm神经缺损,通过大体观察、坐骨神经功能指数恢复率的测定、神经电生理的测定、组织学光镜观察、透射电镜观察、再生有髓神经纤维计数恢复率、神经纤维直径恢复率、髓鞘厚度恢复率的测定等指标评价实验效果。

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