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MethodsNeural tubes isolated from the region between midotic placode and third somite of mouse embryo at ED 8.5d were cultured in DMEM/F12 serum-free medium containing basic fibroblast growth factor and epidermal growth factor. The cell cycle of CNCCs was analyzed with flow cytometry, the proliferation capability was determined by MTT method, AP-2α expression was detected by immunocytochemistry as a biological marker of cardiac neural crest cells. The features of migration, multi-directional differentiation were observed as well.

材料与方法成年2月龄C57/BL6小鼠交配,取ED8.5d小鼠胚胎枕中部至第3体节神经管,应用包含碱性纤维生长因子和表皮细胞生长因子的无血清培养基培养,利用MTT法检测细胞增殖能力,利用流式细胞仪检测细胞周期,采用转录激活因子2α(AP-2α,Activator protein-2α)作为其生物学标记物,观察其增殖状况、细胞周期、迁移、分化等生物学特性。

推荐网络例句

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里,举止就文雅一些,因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计,这件高贵的制服是香港本土制造,是我们第一套不配帽子的制服。

Do not 'inflate' your achievements and/or qualifications or skills .

不要 '夸大' 你的业绩或成果,条件或者技能。