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The lesion, the changes of activity of cholinesterase and acid phosphatase of motor neurons in lateral nucleus of spinal cord anterior horn, the function of axoplasm transportation and nerve conduction, the regeneration of axons and myelin sheath, and the recovery of sciatic nerve function were examined at 7, 14, 30 and 90 days intervals after operation, using Nissl and enzyme histochemistry staining, electrophysiological technique, hydrogen-peroxide oxidoreductase retrograde trace method, axon image analysis, and measurement of sciatic function index. The spinal cord anterior horn of injury side was compared to the correspondence region of spinal cord.

分别于术后7、14、30和90d应用酶组织化学方法、电生理方法、HRP (hydrogen-peroxide oxidoreductase)逆行示踪方法、轴突图象分析方法以及坐骨神经功能指数(sciatic nerve function index,SFI)测量等方法检测坐骨神经损伤后对应脊髓神经元的存活率、神经元胞体酶系变化、损伤神经在轴浆运输、电传导以及轴突、髓鞘再生等方面的恢复情况,探索外周神经损伤后OECs及几丁质对神经元的保护作用以及对新生神经功能恢复的作用,为外周神经损伤的治疗提供新的理论基础。

Neurophysiological basis of cognitive aging lead to degradation, will lead to decline in D.

神经生理的老化导致基础的认知能力退化,将导致晶体智力下降 D。

The majority of clinical studies using intraoperative neurophysiologic monitoring in spinal trauma focus exclusively on somatosensory-evoked potentials, and there are no specific article on the use of transcranial motor-evoked potentials, and stimulated electromyography by direct stimulation of the pedicular screws in thoracolumbar burst type fractures.

研究背景概述:大部分脊髓创伤患者术中神经生理监测的临床研究均集中于体感诱发电位,尚未发现专门的文献采用经颅运动诱发电位、胸腰段爆裂型骨折术中通过直接刺激椎弓根钉诱发肌电图来行脊髓神经生理监测。

Methods Employing cholera toxin B-subunit combined horseradish peroxidase retrograde tracing technique and cholera toxin B-subunit combined colloid goldretrograde tracing with calcitonin gene-related peptideimmunohistochemical double-labeled staining technique,the innervation of musculus sacrococcygeus ventralis medialisin rats was studied.

大鼠骶尾腹内侧肌又称荐尾腹侧中肌(musculus sacrococcygeus ventralis medialis)或屈尾短肌,是位于盆腔后壁、骶椎前方的一对梭形肌肉,属尾肌群,因其功能似乎仅为屈尾,故至今尚未引起人们的注意,而与该肌位置关系较密切的会阴肌因在某些运动神经元疾患时仍可保持其正常生理功能而一直受到神经工作者的关注,尤其是近年来有诸多文献报道了会阴肌的神经支配、相关神经元的细胞构筑、神经递质和神经调质的分布以及纤维联系特点[1~10],藉以探讨运动神经元疾病的神经病理学。

The adipose derived stem cells of inbreds train of rat wereinduced to differentiate into schwann-like cells and the cells were injectedinto the acellular nerve allograff.The proliferation or adhersion of these cells on the graft were observed by inverted microscope or ScanningElectronic Microscope. Activity of cells were detected using MTT. 10mmnerve gap of inbreds train of rats in two group were bridged by tissue-engineered peripheral nerve or autogenous nerve, the effect wereappraised by naked eye, recovery rate of sciatic function index,nerve-electrophysiological, histology, Transmission ElectronMicroscopy and quantitative analysis of recovery rate of myelinated fiberpopulations,diameter of myelinated fiber and thickness of myelin sheat.

按照前述方法培养并诱导近交系大鼠脂肪干细胞向类Sehwann分化,将诱导分化的脂肪干细胞悬液注入已制备的去细胞同种异体神经支架管中,倒置显微镜观察细胞生长情况;扫描电镜下观察细胞在材料上附着情况;四唑盐比色试验测定细胞活性;取两组近交系大鼠,分别用组织工程化神经和自体神经桥接10mm神经缺损,通过大体观察、坐骨神经功能指数恢复率的测定、神经电生理的测定、组织学光镜观察、透射电镜观察、再生有髓神经纤维计数恢复率、神经纤维直径恢复率、髓鞘厚度恢复率的测定等指标评价实验效果。

Materials and Methods 46 cases with brachial plexus injury underwent traditional spinal accessory nerve transfer to restore shoulder abduction were followed-up. Recipient nerve: suprascapular nerve in 35 cases (upper partial brachial plexus injury 21 cases and total brachial plexus injury 14 cases), axillary nerve in 11 cases(upper partial brachial plexus injury 5 cases and total brachial plexus injury 6 cases); And trapezius muscle function was measured by physical examination and electrophysiological study in 18 patients.

材料与方法臂丛神经损伤患者46例,传统锁骨上前入路副神经移位恢复肩关节外展功能;受区神经:肩胛上神经35例(上臂丛神经损伤21例,全臂丛神经损伤14例),腋神经11例(上臂丛神经损伤5例,全臂丛神经损伤6例);术后随访肩关节外展功能的恢复,并对来院随访患者随访检查其斜方肌各部肌力及电生理的变化。

We observed the changes of the proprioceptors in the partly injuried ACL and the xenogeneic graft/artificial graft being used to reconstructing the injuried ACL with gold chlorid staining method and laser copolymerization electron micrography method to observe the morphological changes of the neuromechanism in the ligament tissue,with RT-PCR method to evaluate the proportion and the regeneration of the nerve component and with HRP retrograde tracking technology and electrophysiologic study to judge the functional changes of the neuromechanism in the ligament tissue,respectively.

本研究分别制作兔ACL部分损伤动物模型、异种肌腱移植物重建损伤ACL动物模型和LARS人工韧带重建损伤ACL动物模型,分别于术后不同时间点对损伤ACL/移植物进行氯化金染色光学显微镜检查和PGP9.5免疫荧光染色后激光共聚焦电子显微镜检查观察韧带内神经结构形态学变化,用RT-PCR法检测韧带中GAP-43和PGP 9.5的mRNA表达情况以评价损伤ACL/移植物内神经成分比例及神经再生情况,通过HRP逆行追踪技术和电生理检查(包括体感诱发电位和腘绳肌肌电图)评价损伤ACL/移植物内神经结构的功能情况。

Objective The changes of some neuropeptides were observed in brain slices of rats with spinal cord damage. Methods The experimental rats were divided into five groups:spinal cord occupied,contused,transected,false-operation groups and normal control.24 hours after operation,all animals were sacrificed,and brain slices produced.

随着神经系统神经肽的发现,针对神经肽功能以及通过神经肽变化来明确神经系统病理生理机制的研究越来越多[1];因而,在脊髓损伤领域,神经细胞继发性改变中神经肽的作用也有许多研究[2],但脊髓损伤后大脑中枢神经肽的改变尚无文献报道。

Application of neuro-physiological prefading in CSEA during transurethral vaporization of the prostate on aged patients had a lot of advantages. The security was more reliable. It had little influence on patients. It was the optimal method of anesthesia.

神经生理预调节在高龄患者前列腺电切术脊麻-硬膜外联合麻醉中的应用增加了机体稳定性,生理影响小,提升了CSEA在高龄患者中应用安全性。

We report a GBS patient who after receiving an operation for polycystic liver disease under general anesthesia developed weakness of upper and lower extremities on the 3rd postoperative day, with decreased deep tendon reflex, which ultimately evolved into respiratory failure. Slurred speech and bilateral ptosis were also noted. All these manifested an acute peripheral polyneuropathy combined with bulbar involvement. According to the clinical picture, CSF examination and results of nerve conduction tests, Guillain-Barré syndrome was diagnosed.

一病例由於肝脏的多囊性疾病接受手术治疗,在手术后三天病人出现四肢无力的症状,深部肌腱反射也降低,最后发生呼吸衰竭的情况,这是一急性多发性的周边神经病变,经过脑脊髓液和神经生理的检查后,诊断为一Guillain-Barré syndrome的病例。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。