神经形成

Methods: The bilateral superior buccal branches of facial nerve were exposed and transected, a nerve growth chamber were created by suturing the epineurium and silicone chamber under microscope .The chambers of experimental sides were injected with HGF/normal saline and that of the control sides with normal saline of same amount (10μL ),the regenerated nerves in the chambers were dissected and postoperative evaluation were performed byelectrophyiological testing , histopathologic, morphological studies under gross observation, light microscope at 1,2,4 weeks after operation, respectively.

实验设生理盐水对照组和肝细胞生长因子组，选用30只成年、健康新西兰家兔，暴露并分离双侧面神经上颊支，将其横断后套入硅胶管内，用显微外科技术将硅胶管与神经外膜缝合以形成硅胶神经再生腔，在一侧硅胶神经再生腔内注入HGF为实验侧，在另一侧注入生理盐水为对照侧，于术后1周、2周、4周分别麻醉家兔后行电生理检测，在肉眼、光镜、特殊染色下进行组织形态学观察，并在光镜下进行形态定量分析。

Anatomical study proved:①.4~8 nutritional vessels of sural nerve –small saphenous vein were the intermuscular perforators of peroneal artery, the furthermost one located at(1.0±1.3)cm above the tip of external malleolus with diameter (0.6±0.2)mm;②.2~6 musculocutaneous perforators were given off by the artery of outer head of gastrocnemius muscle to supply nutrition to the sural nerve-small saphenous vein;③.intermuscular perforators from peroneal artery supplied blood to fibula and outer head of soleus muscle.the perforators with an average outer diameter of （1.3-1.6）cm gave off cutaneous branch ,fascial branch and nutritional vessels to the sural nerve-small saphenous vein,which formed the vessel chain of the sural nerve-small saphenous vein ,sub-fascial and suprafascial vessel net.

结果：1、解剖实验证实：①、腓肠神经-小隐静脉营养血管来自腓动脉肌间隔穿支4～8支，最远的跟外侧动脉穿支距外踝尖上(1.0±1.3)cm，外径(0.6±0.2)mm。②、腓肠肌外侧头动脉沿途发2-6支的肌皮穿支营养腓肠神经-小隐静脉。③、腓动脉肌间隔穿支营养腓骨、比目鱼肌外侧半。上述穿皮支平均外径在0.3～1.6mm，发出皮支、筋膜支、腓肠神经-小隐静脉营养血管，形成腓肠神经-小隐静脉血管链以及深、浅筋膜血管网。

Neuropharmacology is a basic science and forms general neuroscience together with neuro-anatomy, neurochemistry, neuro-physiology, etc.

神经药理学是一门基础科学，和神经解剖学，神经化学，神经生理学等共同形成了综合性的神经科学。

Results:(1)NSCs form typical neurospheres under adequate concentration in vitro, which are immunoreactive to Vimentin. Typically and terminally differentiated mature neural cells could not be found without the stimulus of mitogen or only under NSCs self-regulation and self-induction;(2)NSCs derived from hippocampus maintain the character of stem cells much longer with better biological behavior; NSCs passed to the 2-3 passage are the best to graft since they have not differentiated;(3)NSCs cultured in vitro could self-regulate and differentiate into neurospheres and progenitors positively immunoreactive to specific antibodies representing neurons, astrocytes, oligodendrocytes and Schwann cells;(4)There are widespread synaptic contacts between various kinds of descendent clones and cells;(5)Neurospheres could be formed without the stimulus of mitogen when NSCs and OECs are cocultured. Many neurospheres and cells immunoreactive to Vimentin, GFAP, MAP2, 02, p75NGFR, GFAP, S-100, Synaptosis, Vimentin, Tau (Tau is only positive in cocultureof HNSCs+HOECs) could be found;(6)The supernatant fluid triturated from adult rat spinal cord stimulates NSCs to differentiate into neurons, but do not terminally differentiate;(7)Fibroblasts and O4 oligodendrocytes are not supported to grow under this culture medium.Part II: Isolation, culture and identification of rat and human olfactory ensheathing cellsOlfactory ensheathing cells/glials are the most powerful cells to enable the regeneration of axons in the central nervous system.

结果表明：①在适宜的浓度体外培养条件下，NSCs能形成典型的神经干细胞克隆球，Vimentin免疫荧光染色阳性，单靠丝裂原刺激或NSCs自我调节和分化诱导，不会产生典型的终末分化的成熟神经细胞；②海马源性的NSCs维持干细胞特性的时间更长，生物学特性更优；③传至第2～3代的NSCs尚未分化时移植最佳；④体外培养的NSCs能自我调控分化为神经元、星形胶质细胞、O2少突胶质细胞、雪旺氏细胞染色阳性克隆球和前体细胞；⑤各种子代克隆球和细胞存在广泛的突触联系；⑥NSCs与OECs联合培养时，不需丝裂原刺激即能形成克隆球，获得大量Vimentin、GFAP、MAP2、O2、p75NGFR、GFAP、S-100、Synaptosis、Vimentin、Tau(Tau只有人HNSCs+HOECs联合培养时出现阳染)染色阳性的克隆球和细胞；⑦脊髓研磨后的上清液刺激神经干细胞向神经元方向分化，但并不出现终末分化；⑧本研究培养条件不利于成纤维细胞、O4生长。

It is interesting that cervical root avulsion is a relatively frequent cause of superficial siderosis of the CNS. It is possible that trauma to the nerve root damages the fine venous plexus within the root, or the medullary and radicular veins that run along the nerve root, resulting in a fragile vascular scar.

很有意思的是颈神经根撕脱也是相对比较常见的病因之一，可能因为神经根的创伤可损伤神经根处的静脉丛及沿着神经根走行的根静脉，导致脆性血管损伤形成疤痕，当屈伸颈部时因牵引而破裂出血。

We introduced improved primary mixed glial culture and different-attachment method to isolate and purify the OPCs, the cells were proliferated in serum-free medium, flow cytometry and immunohischemistry methods were employed to estimate the purity of cultured OPCs. Their abilities of differentiation and expression of trophic factors were identified by RT-PCR and immunostaining. Several methods including TUNEL and MTT were adopted to estimate the protective effects of conditioned culture medium from oligodendrocyte lineage cells on the primary cultured cerebellar granule neurons. Intravitreal transplant of OPCs, combined with retrograde fluorescent labeling the superior colliculus and intraorbital optic nerve transection, were used to investigate the protective effects of OPCs on the axotomized RGCs in vivo. Intravitreal transplantof OPCs or NSCs on the newborn rats, and retinal transplant of OPCs on the young rats were performed, to observe the myelin formation in the retina at different stages after cellular transplantation. Optic nerve transection was carried out on some rats with myelinated retinae, to study the influence of myelination on the injuried RGCs.

为此，本研究采用改良的胶质细胞混合培养与差速贴壁方法获得大鼠OPCs，使用无血清培养基进行扩增、培养，用免疫组织化学和流式细胞技术对培养细胞的纯度进行鉴定，对少突胶质系细胞表达部分营养因子的情况进行检测；采用TUNEL、MTT等方法对少突胶质系细胞条件培养基对原代培养小脑颗粒神经元的保护作用进行检测；将OPCs移植入成年SD大鼠玻璃体内，利用上丘逆行荧光标记技术，观察眼内移植的OPCs对眶内视神经切断时的视网膜神经节细胞的保护作用及其持续时间；将OPCs或NSCs移植入新生和幼年SD大鼠玻璃体或视网膜内，观察不同时期视网膜内髓鞘形成与分布特点，分析髓鞘的超微结构，并观察眼内髓鞘形成对损伤神经节细胞的保护作用。

METHODS: We performed neurological examination, neurophysiological, neuroimaging, and neuropathological studies on sural nerve biopsy in 10 hypomyelination and congenital cataract patients from 5 unrelated families.

我们进行神经系统检查、神经生理学、神经影像学和腓肠神经活检的神经病理学研究，这些是对来自于5个不相关家庭的10例先天性白内障和神经髓鞘形成不良患者进行的检查。

The study suggested that TNF〓 and IL-1〓 might be produced in the cascade of events resulting in Wallerian degeneration and hyperalgesia following peripheral nerve compression, and increased TNF〓 and IL-1〓 mRNA may trigger other later responses in nervous system contributing to the initiation of peripheral neuropathic pain or other degeneration/regeneration processes.

提示：外周神经受压后诸如华勒氏变性、痛觉过敏等一系列反应可能有TNF〓和IL-1〓的产生，而后者的增加又会启动其它神经反应而在外周神经源性痛形成和神经退变/再生中发挥作用。

Several investigators have determined that placing the electrode parallel rather than perpendicular to the target nerve substantially increases the size of the lesion, thereby reducing the likelihood the treatment will miss or only partially coagulate the target nerve.234,235 After a literature review and cadaveric study, Lau et al.234 concluded the ideal electrode position is across the lateral neck of the superior articular process rather than the groove formed at the angle of the superior articular and transverse processes, as was used in most studies.159,160Other investigators have found the maximal lesion size to be reached within 60 s of lesion time,235–237 independent of whether the system is temperature or voltage controlled.238 Studies conducted in human myocardium have determined that irrigation fluid has either no effect or a slightly beneficial effect on lesion size.239 Hence, the use of LA to prevent procedure-related pain or steroid to reduce the incidence of neuritis184 should theoretically have no adverse effects on the efficacy of radiofrequency denervation.

一些研究者认为，将点击平行的置于目标神经比将其垂直置于目标神经增加了消融的范围，因而减少损伤可能使目标神经不被或部分被消融。Lau等回顾了文献中对尸体的研究，总结出理想的电极放置位置为上关节突颈部外侧而非常采用的上关节突与横突形成的沟。其他学者研究发现，不论系统是温度控制或电压控制，其最大的损伤范围都在60秒内达到。在心肌灌注的研究中，灌注液多损伤范围造成轻微或不造成损伤。因此，使用LA预防过程相关性疼痛或类固醇防止神经炎发生时，需理论上确保对射频消融效果无副作用。

Neural stem cells have a strong self-renew mechanism and it can transform after a little break. Neural stem cells have a long term survival, which mean that it has more probability of wrong copy than mature cells. These cells are formed glioma stem cells in the end. The genes who adjust neural stem cells can express in glioma stem cells, which hold out glioma stem cells from neural stem cells. There is another presume that glioma stem cells come from differentiated cells. Through the gene break of these cells, they can obtain characteristics of stem cells, then form glioma stem cells.

神经干细胞具有很强的自我更新机制，获得较少突变即有可能恶性转化，而且干细胞存活时间较长，这意味着干细胞比成熟细胞发生细胞复制的错误几率更大，因外界环境的刺激而发生突变的机会更多，最终形成脑胶质瘤干细胞，同时调节神经干细胞增殖和自我更新的基因在脑胶质瘤的脑胶质瘤干细胞中也表达，这也是支持神经干细胞是脑胶质瘤干细胞来源的；也有推测认为它可能起源于已分化的细胞，由这些细胞突变发生去分化得来，并通过基因突变而获得了干细胞自我更新的特性，从而形成脑胶质瘤干细胞。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。