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After 6 to 8 weeks behavioral test, electrophysiologic test, muscle weight measurement of flexor carpi ulnaxis and abductor digiti minimi manus, and ultrastructural observation were carried out.

对120只Wistar大鼠在上臂肌皮神经人肌水平不同程度切断尺神经(分别切断1/6,1/3,1/2,2/3及全部),术后6~8周观察大鼠的行为变化,尺神经电生理变化,尺侧腕屈肌及小指展肌的肌湿重比及肌细胞超微结构的改变。

Objective To study changes of expression in Ephrin and its receptors in DRG neurons after peripheral nerve injury.

目的 研究外周神经损伤后背根神经节细胞中Ephrin B1及其相关受体的表达变化。

After 14 weeks of operation, the structure of epineurium was in integrity and there was no obvious difference in perineurium and endonurium between two groups.

结果 术后 2周,A组炎性反应稍重于 B组;至 4周时两组的炎性反应程度相似,近端少许胶原纤维增生;8周时两组的炎性反应基本停止,胶原纤维增生稍明显;14周时两组神经外膜构成完整,束膜、内膜结构无明显差异。

Results: Current of 1 mA, 210 s could cause necrosis and cavitation in the dorsal funiculus, resulting in bilateral corticospinal tract injury; rats with electrolytic lesions exhibited declined motor function with decreased Tarlov score, increased Ashworth score and reduced inclined plane angle, the HRP labeled neuron counts in motor cortex were also significantly lower in the lesioned group than sham-operation group.

结果:1 mA, 210 s的损伤电流,可在脊髓后索造成神经组织坏死,形成空腔,使双侧皮质脊髓束纤维受损;术后1周、2周、3周观察,电损毁组大鼠运动功能显著低于假手术组,表现为后肢运动评分下降,Ashworth 肌张力评分增大,斜板实验角度下降;电损毁组运动皮质标记神经元计数也显著低于假手术组。

Seizures and focal neurologic signs, including cranial-nerve palsies, are common, as are peripheral-blood leukocytosis and hyponatremia.

癫痫样发作和局部神经体征,包括颅神经麻痹,以及外周血白细胞增多和低钠血症都是通常所见的表现。

Figure 2 Cranial nerve susceptibility in superficial siderosis of the central nervous system in relation to glial length. Relative length of the glial and peripheral segments of each cranial nerve. Open bar, peripheral nerve; closed bar, glial component. Adapted from Tarlov 1937

图2。 中枢神经系统表面含铁血黄素沉积症中颅神经的易感性与神经胶质长度有关,下图显示了每条颅神经中心部分神经胶质长度与外周组成部分的相对比例。

Electromyographic, histological and ultramicroscopic analysis were performed in 2, 8 and 16 weeks postoperatively. The nerve conduct velocity, amplitude of compound muscle action potential, myelinated nerve fiber counts and ultramicrostructure were studied and processed statistical analysis.

分别于术后2、8、16周对再生神经及正常对照行电生理、组织学及超微结构观察,分析并比较神经传导速度、复合肌肉动作电位、神经纤维计数、神经超微结构等指标,行相应的统计学处理。

Methods:plasmatic neuropeptid y concentration was determined with radioimmunoassay in 38 schizophrenia patients and 20 normal controls.results: compared with normal controls, plasmatic neuropeptid y concentration in schizophrenia patients was significant decreased (t=9.49,p.01).there were significant difference in plasmatic neuropeptid y of patients before and after clozapine treatment (t=2.20,p.05).conclusions: the results suggest that there are abnormal metabolism in plasmatic neuropeptid y of schizophrenia patients .

采用放射免疫法测定38例精神分裂症患者及20例正常对照者的血浆神经肽y含量。结果:精神分裂症患者血浆神经肽y含量较对照组低,差异非常显著(t=9.49,p.01)。精神分裂症患者经氯氮平治疗4周,患者症状好转后,其血浆神经肽 y含量较治疗前显著升高(t=2.20,p.05)。

Gelatin microsphere is firstly made by encapsulating bFGF into gelatin by special manufacture process, then this microsphere was combinated into PLGA conduit or PLGA film.when used, we need only pack the injury peripheral nerve, injury spinal cord, wounded pars encephalic with the PLGA conduit. The conduit or film can slowly release buff which would be absorbed by around tissues.bFGF was already proved to be nutritious to the regeneration and alive of neuron and protect injury neuron.bFGF can also promote morphogenesia and division of nerve cell which would raise the survival rate of neuron, guide the generation of axon etc.bFGF is one of the important factors of sustaining the normal survival of cholinergic nerve.

使用时,只需将此材料贴敷外周损伤神经伤患处,脊髓伤口,或在脑部开颅手术后填充于术后创面,可有效地在伤口局部缓慢地释放并由机体组织吸收bFGF等神经营养因子。bFGF等已被证明具有神经营养作用,可促进神经元的再生与存活,保护受损的神经元。bFGF等对神经细胞兼有促进形态发生与分裂作用,具有提高神经元的成活率、诱导轴突向外生长等功能,是维持前脑基底部胆碱能神经元正常生存的重要因素。

This experiment selects material E14d SD the big mouse SVZ nerve stemcell as well as P7d the SD big mouse internal ear stem cell, does nothave the blood serum complete culture medium raise through D-MEM/F12to cause its massive multiplication, a part carries on Nestin, NF,CNP, GFAP, MAP-2, Myosin, the P27 immunity group, proved the raise thenerve stem cell has many to the split up ON potential; Another partdyes EGFP after the extension to carry on the guinea pig internal earto transplant, after two weeks fills the class to take the cochleafixed slice observation, discovered has the stem cell ball group inthe internal ear survival, proved the nerve stem cellheterotransplantation the possibility and for transplants the stemcell migration differentiation repair hair cell damage the thoroughresearch accumulation experiment experience.

本实验取材E14d SD大鼠SVZ神经干细胞以及P7d SD大鼠内耳干细胞,通过D-MEM/F12无血清完全培养基培养使其大量增殖,一部分进行Nestin、NF、CNP、GFAP、MAP-2、Myosin、P27免疫组化,证明培养的神经干细胞具有多向分化的潜能;另一部分通过转染EGFP后进行豚鼠内耳移植,两周后灌流取耳蜗固定切片观察,发现有干细胞球团在内耳存活,证明神经干细胞异体移植的可能性并为移植干细胞迁移分化修复毛细胞损伤的深入研究积累实验经验。

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