神经分布

METHODS:The distribution changes of N CAM at flatfish muscle neuromuscular junction of rat in its embryon phase,14 day after birth,adulthood and 10,20 and 30 days after its L5 lumber nerve root injury were observed by immunohistochemistry and confocal laser scanning microscopy technologies.

采用免疫组织化学方法和激光共聚焦显微扫描技术，观察了大鼠胚胎期，出生后14d，成年期以及L5神经根受压后10，20，和30d，大鼠比目鱼肌神经肌肉接头部N-CAM的分布变化。

Microanatomical dissection of the median nerve was done to observe the distribution of fascicular groups of the median nerve in the upper arm.

对34侧成人上肢标本的臂部正中神经内神经束组的分布作了显微外科观察。

The distribution of intraneural fascicular groups should be concerned when partial transfer of median nerve in the upper arm was done.

在臂部采用正中神经部分束移位时，要了解神经干内束组的分布特点。

Methods: animals were randomly classified into 5 groups: group a, gaster poured by physiological solution; group b, gaster poured by ethanol; group c, gaster poured by ethanol after hypophrenic vagectomy; group d, gaster poured by ethanol after big small splanchnicotomy; group e, gaster poured by ethanol after hypophrenic vagotomy and big small splanchnicotomy.the distribution of c fos positive neuron in central nervous system was displayed and immunohistochemical staining method was used.

取30只成年雄性wistar大鼠，随机分为5组，每组6只。a组：单纯生理盐水灌胃组；b组：单纯乙醇灌胃组；c组：膈下迷走神经切断乙醇灌胃组；d组：内脏大、小神经切断乙醇灌胃组；e组：膈下迷走神经和内脏大、小神经均切断乙醇灌胃组。运用免疫组化技术显示fos阳性神经元在大鼠中枢神经系统中的分布。

Five patterns of perineural invasion were observed and 3 nerve plexuses in the hepatoduodenal ligament and Glisson sheath were found.

肿瘤浸润神经有5种方式，肝十二指肠韧带与一、二级肝管所在的Glisson鞘内分布的神经丛主要有3丛。

Methods Pathological sections from 68 patients with hilar cholangiocarcinoma were observed by microscope and the distribution of nerve plexuses in normal hepatoduodenal ligament was analyzed based on the observation of 40 histological sections from 5 autopsy cases.

观察68例肝门部胆管癌患者的病理切片，总结归纳肿瘤神经浸润的方式和规律；分析5例尸检标本正常肝十二指肠韧带内的神经丛分布特点。

We performed a study to determine the actual location of local anesthetic when ilioinguinal/iliohypogastric nerve blocks are performed using landmark-based techniques in children in an attempt to explain a failed block.

我们做了一项研究来观察在定位技术下儿童髂腹下/髂腹股沟神经阻滞时局麻药的实际分布位置，试图以此来解释无效的神经阻滞。

We introduced improved primary mixed glial culture and different-attachment method to isolate and purify the OPCs, the cells were proliferated in serum-free medium, flow cytometry and immunohischemistry methods were employed to estimate the purity of cultured OPCs. Their abilities of differentiation and expression of trophic factors were identified by RT-PCR and immunostaining. Several methods including TUNEL and MTT were adopted to estimate the protective effects of conditioned culture medium from oligodendrocyte lineage cells on the primary cultured cerebellar granule neurons. Intravitreal transplant of OPCs, combined with retrograde fluorescent labeling the superior colliculus and intraorbital optic nerve transection, were used to investigate the protective effects of OPCs on the axotomized RGCs in vivo. Intravitreal transplantof OPCs or NSCs on the newborn rats, and retinal transplant of OPCs on the young rats were performed, to observe the myelin formation in the retina at different stages after cellular transplantation. Optic nerve transection was carried out on some rats with myelinated retinae, to study the influence of myelination on the injuried RGCs.

为此，本研究采用改良的胶质细胞混合培养与差速贴壁方法获得大鼠OPCs，使用无血清培养基进行扩增、培养，用免疫组织化学和流式细胞技术对培养细胞的纯度进行鉴定，对少突胶质系细胞表达部分营养因子的情况进行检测；采用TUNEL、MTT等方法对少突胶质系细胞条件培养基对原代培养小脑颗粒神经元的保护作用进行检测；将OPCs移植入成年SD大鼠玻璃体内，利用上丘逆行荧光标记技术，观察眼内移植的OPCs对眶内视神经切断时的视网膜神经节细胞的保护作用及其持续时间；将OPCs或NSCs移植入新生和幼年SD大鼠玻璃体或视网膜内，观察不同时期视网膜内髓鞘形成与分布特点，分析髓鞘的超微结构，并观察眼内髓鞘形成对损伤神经节细胞的保护作用。

The basal laminae on each side of perineural cells were thickened and incomplete. Microfibrils were compressed and scattered at the periphery of the nerve.

神经周围细胞两侧的基底板增厚且不完整；微纤维散乱地分布在神经的周围且呈被压缩的状态。

At 14 days after sciatic nerve torsion injury, all rats were anaesthetized again and the distal section of sciatic nerve was obtained and cut into slices, meanwhile the cross sectional area, the density and size frequency distribution of medullated fibers, as well as the number of medullated fibers in each nerve were determined.

坐骨神经捻挫损伤后14 d，全部动物再次麻醉，取捻挫部位远端坐骨神经制备切片，测量并计算神经横截面积，有髓纤维密度和有髓纤维大小频率分布和每根神经的有髓纤维数目。

The absorption and distribution of chromium were studied in ryeusing nutrient culture technique and pot experiment.

采用不同浓度K2CrO4(0，0.4，0.8和1.2 mmol/L)的Hoagland营养液处理黑麦幼苗，测定铬在黑麦体内的亚细胞分布、铬化学形态及不同部位的积累。

By analyzing theory foundation of mathematical morphology in the digital image processing, researching morphology arithmetic of the binary Image, discussing two basic forms for the least structure element: dilation and erosion.

通过分析数学形态学在图像中的理论基础，研究二值图像的形态分析算法，探讨最小结构元素的两种基本形态：膨胀和腐蚀；分析了数学形态学复杂算法的基本原理，把数学形态学的部分并行处理理念引入到家实际应用中。