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The effective stimulating sites were indentified by defensive cardiovascular response and other components of defence reaction evoked by 3 s trains of rectangular cathodal pulses (100Hz, 0.5ms pulse duration, 70 -150μA intensity for dPAG and 150-250μA for PF) delivered from a programmed multichannel constant current stimulator. The responses included an increase in blood pressure and heart rate, pupillodilation and an increase in the rate and depth of respiration. Currents of 1Hz, intensity of 100- 150μA for dPAG and 200-250μA for PF were used to observe the effect of the stimulation of them on RVLM neurones. The deep peroneal nerve and superfacial deep peroneal nerve were carefully separated, and put on a pair of stimulating electrode respectively, Both nerves were stimulated with constant rectangular pulses of 1Hz, 0.5ms duration and 300μA current intensity to see their effects on RVLM neurones. The baro-and chemo-receptors were activated by the intravenous injection of phenylephrine or 0.208 M NaH2PO4. The degree of convergent inputs to individual RVLM neurone was studied by the stimulation of the sites mentioned above. It was found that neurones about 61.4%(27/44) and 35.3%(6/17) received convergent inputs from three and four places respectively.

过去的研究主要集中于整体水平,利用电刺激或化学刺激RVLM神经元,观察对血压、心率及交感活动的影响,并对影响心血管交感活动的神经递质进行了广泛探讨,积累了大量资料;在神经元水平,主要研究刺激脑内核团、外周神经、化学感受器及压力感受器等对RVLM神经元放电的影响和某些神经递质影响神经元活动的机制、然而,对RVLM神经元如何整合不同信息、其可能突触机制等研究得较少,本互作拟采用在体细胞内、细胞外记录电活动与微电泳,结合中枢核团及外周神经电刺激等方法,对RVLM神经元在不同状态下的整合作用规律与可能机制进行初步探讨,为进一步了解这一复杂的整合过程提供新的理论依据。

Methylene blue-basic magenta staining and electron microscopy Regenerating nerve were arranged in bundles in group A and B, there were minifascicle formation and a large amount of proliferated collagen in between the minifascicled, no cicatrices form, regenerating vasa spread randomly, electron microscopy show a large number of myelinated and unmyelinated fiber arranged in buddies.

亚甲基蓝-碱性品红染色及电镜观察两组均见再生神经纤维呈束状分布,有多个神经束,每一个神经束内见大量密集、粗大的有髓神经纤维,神经束之间充满增生的胶原,未见瘫痕形成,再生血管散在分布。

Continence nerves of female urethra contained the branches of pelvic nerve, intrapelvic and extrapelvic branches of pudendal nerve.

女性尿道的控尿神经是由盆神经和阴部神经的盆内盆外分支组成。

METHODS: The spiral modiolar artery in guinea pig cochlea was exposed by microsurgery, then the fluorogold or horseradish peroxidase was given around the SMA for retrograde tracing.

在正常豚鼠单侧耳蜗螺旋蜗轴动脉局部滴加逆行追踪剂辣根过氧化物酶或荧光金,观察双侧交感颈上神经节、星状神经节内HRP或FG逆标神经元的分布;采用逆标与免疫组化相结合的双重标记方法观察追踪阳性神经元是否表达酪氨酸羟化酶、神经肽Y、钙基因相关肽或P物质。

Methods: the spiral modiolar artery in guinea pig cochlea was exposed by microsurgery, then the fluorogold or horseradish peroxidase was given around the sma for retrograde tracing. the bilateral superior cervical ganglia and stellate ganglia were observed to find retrogradely labeled neurons. the neurotransmitters of these sympathetic fibers around sma were ascertained by using retrograde tracing combined with immunohistochemical double labeled technique.

在正常豚鼠单侧耳蜗螺旋蜗轴动脉局部滴加逆行追踪剂辣根过氧化物酶或荧光金,观察双侧交感颈上神经节、星状神经节内hrp或fg逆标神经元的分布;采用逆标与免疫组化相结合的双重标记方法观察追踪阳性神经元是否表达酪氨酸羟化酶、神经肽y、钙基因相关肽或p物质。

The result showed that, the body wall most outer layer cuticle assumesthe translucent porodine, the surrounding entire body wall; Close isconnected with the cuticle 表皮层 for 合胞体 the structure, inthe back, the abdomen and its two sides separately adds thick to inprominent Cheng Jizhuang, separately contains the back side,腹侧the nerve and compares to the developed back, the abdomen nerve; Themyo- position to the body wall most inner layer, has the muscle cellto arrange becomes; Between the body wall and the digestive tractcavity is a body cavity; Digestive tract for 肌质 structure, afterinference for roundworm's esophagus.

结果显示,体壁最外层的角质层呈半透明的胶状,包围整个体壁;与角质层紧密相连的表皮层为为合胞体构造,在背、腹及其两个侧面分别加厚向内突出成脊状,分别包含背侧、腹侧神经和相比对发达的背、腹神经;肌层位于体壁最内层,有肌细胞排列而成;体壁与消化道之间的空腔为体腔;消化道为肌质结构,经推断为蛔虫的食道。

Results 5 cases of ependymocytoma, 3 cases of astrocytoma, 1 medulloblastoma hemangioblastoma in 10 cases of intra-medullary tumor; 36 cases of neurogenic tumor, 11 cases of spinal meningioma in 47 cases of subdural extramedullary tumor; 2 cases of neurogenic tumor strided over scleromeninx;2 cases of extradural metastatic tumor, 1 cestodiasis, 2 cases of epidural hematoma, which were mistaken for hemangioma before surgical operation and 1 vascular malformation in 4 cases of intraspinal nontumorous occupying lesion.

结果:10例髓内肿瘤中,室管膜瘤5例;星形细胞瘤3例;血管母细胞瘤1例;成神经管细胞瘤1例。47例脊髓外硬膜下肿瘤中,神经源性肿瘤36例;脊膜瘤11例。2例跨硬膜内外生长的神经源性肿瘤2例。硬膜外的转移瘤2例。4例椎管内非肿瘤性占位性病变中,椎管内绦虫病1例;硬膜外血肿2例,术前误为血管瘤;血管畸形1例。

Several investigators have determined that placing the electrode parallel rather than perpendicular to the target nerve substantially increases the size of the lesion, thereby reducing the likelihood the treatment will miss or only partially coagulate the target nerve.234,235 After a literature review and cadaveric study, Lau et al.234 concluded the ideal electrode position is across the lateral neck of the superior articular process rather than the groove formed at the angle of the superior articular and transverse processes, as was used in most studies.159,160Other investigators have found the maximal lesion size to be reached within 60 s of lesion time,235–237 independent of whether the system is temperature or voltage controlled.238 Studies conducted in human myocardium have determined that irrigation fluid has either no effect or a slightly beneficial effect on lesion size.239 Hence, the use of LA to prevent procedure-related pain or steroid to reduce the incidence of neuritis184 should theoretically have no adverse effects on the efficacy of radiofrequency denervation.

一些研究者认为,将点击平行的置于目标神经比将其垂直置于目标神经增加了消融的范围,因而减少损伤可能使目标神经不被或部分被消融。Lau等回顾了文献中对尸体的研究,总结出理想的电极放置位置为上关节突颈部外侧而非常采用的上关节突与横突形成的沟。其他学者研究发现,不论系统是温度控制或电压控制,其最大的损伤范围都在60秒内达到。在心肌灌注的研究中,灌注液多损伤范围造成轻微或不造成损伤。因此,使用LA预防过程相关性疼痛或类固醇防止神经炎发生时,需理论上确保对射频消融效果无副作用。

Methods: Dorsal root ganglions were derived from the chick embryo and dispersed into single neuron cell,The rhCNTF was added to neuron cells and incubated for 64 hours,The activity of acid phosphatase in neuron cells was determined and the biological activity of rhCNTF was analyzed quantificationally.

从鸡胚中分离出背根神经节并制成神经细胞,将重组人睫状神经营养因子加入到细胞中继续培养64h后,用酸性磷酸酶法检测活细胞内酸性磷酸酶的活性,从而定量测定重组人睫状神经营养因子的生物活性。

When the effect ofNTP was examined after depletion of monoamines in the spinalcord by intrathecal neurotoxins, the antiallodynic and antihyperalgesiceffects were still observed after serotonergic denervation,but not after noradrenergic denervation.

鞘内给予神经毒素耗竭脊髓单胺递质后检查 NTP 的作用时,去除5羟色胺能神经支配后 NTP 的抗触觉性痛感和抗痛觉过敏作用仍然存在,但是去除去甲肾上腺素能神经支配后则消失。

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