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First neurotrophic factors could be concentrated within nerve conduits. Second fibre-bridge to anastomose two parts of nerve gaps could be formed ecxactly. Third operation of nerve conduits could be transplanted easily.

神经导管一方面能使神经营养因子在导管内积聚,浓度增高,另一方面引导神经缺损两端准确对合,同时导管的植入操作简单,可以减少手术对神经的损伤。

MethodsWe chose 20 adult cadavers' lower limbs, dissected the MCNs, observed their original sites, shapes, courses and distribution, and measured the perpendicular distance from original sites of MCNs from tibial nerve, original sites of their branches to the tip of medial malleolus, and the external diameters of their main trunks and branches. ResultsThe frequency of the MCN was 95% in this array.

选择成人尸体标本20个下肢,解剖跟内侧神经,观察其发出部位、形态、走行与分布情况,分别测量并记录跟内侧神经自胫神经发出部位及其属支分支处到内踝尖平面的距离和跟内侧神经主干及其主要分支的外径。

Result bFGF immunoreactivity was found in neuron and gli al cell in corte x,hippocampus habenular nucleus,and found in macrophage surrounding hematoma; Ln immunoreactivity was found in neurons,glial cells and blood vessles in front a l cortex,hippocampus,thalamus hypothalamus.

结果 脑出血后脑内bFGF主要在扣带皮质、海马、缰核及其他区域表达,阳性细胞骨折主要有神经哮喘元、神经哮喘胶质、吞噬细胞骨折、室管膜细胞骨折、伸长细胞骨折等;Ln不仅在大脑皮质、海马、缰核、下丘脑等区域的神经哮喘元表达,且在这些区域的神经哮喘胶质细胞骨折也表达,血管也有阳性反应。

It was achieved by intradural microanastomosis of the L6 ventral root to S2 ventral root. After axonal regeneration, the "patellar ligament-spinal cord center-bladder" reflex pathway was reestablished. The function of the reflex arc was observed by nerve electrophysiological experiment, detrusor electromyography experiment, urodynamic testing and HRP retrograde tracing before and in 8 months after the spinal cord was destroyed horizontally between S1 and S4 level. There was one case of conical SCI patient who had L1 fracture and paraplegia was received intradural microanastomosis of the L6 ventral root to S2 ventral root after Li fracture and paraplegia.

将Beagle犬L6前根近端与S2前根远端在硬膜内显微吻合,经一段时间的轴突再生后,建立&膝腱-脊髓中枢-膀胱&人工反射弧,术后8个月,在破坏S1~S4脊髓节段前后分别进行神经电生理、膀胱肌电图及尿流动力学和辣根过氧化物酶逆行示踪等检查;对1例L1压缩性骨折伴截瘫患者行右侧T11与52前根经腓肠神经移植硬膜内吻合术。

Intrafascicular microelectrodes can stably record the electrical signals of cardiac sympathetic nerve.

神经束内微电极可稳定记录心交感神经电信号,能较好反映神经束内电活动的变化。

Methods: The expression level of NOS and NGF was detected in pterygopalatine ganglion of the allergic rhinitis rats using histochemieal and immunofluorescence staining methods respectively.

建立AR大鼠模型,用HE染色方法观察鼻黏膜内腺体的变化;用组织化学的方法观察翼腭神经节内NOS表达的变化;用免疫荧光的方法观察翼腭神经节内NGF表达的变化。

At postnatal 1st week immunopositive reaction of NGF was detected mainly in sustentacular cells and the spermatogonia also showed positive staining. NGF positive staining in the testes was observed in interstitial cells, spermatogenetic cells, sustentacular cells and Leydig cells at 3rd week. After the postnatal 5th week, NGF-positive immunostaining was also detected in intersitial cells and spermatogenetic cells, but the intensity of reaction was weaker than that at 1st and 3rd weeks.

免疫组化定位分析显示:睾丸组织的神经生长因子蛋白表达于小鼠出生后的各个时期内,1周龄睾丸组织免疫阳性反应主要位于支持细胞,精原细胞也有着色;3周龄睾丸组织的间质细胞、各级生精细胞、支持细胞、管周肌样细胞表达均呈现阳性;5周后的睾丸组织内神经生长因子呈低水平表达,主要表达于间质细胞和生精细胞内。

Morphological features of IND under TEMIn normal neurons, the membrane of cells and nucleus is intact, the cell organs such as mitochondria and endoplasmic reticulum and ribosome are clear, the nucleolus are distinct, the chromatin is loosen, the cytoplasm is filled with neuroendocrine vesicle.

S-100免疫组化结果:神经丛内神经纤维和胶质细胞呈阳性反应,神经元细胞反应呈阴性。IND病变可见巨神经丛内布满大小不一的缺损区,缺损区中央可见发育不良细胞核,神经纤维增生不明显。

Objective To explore the effects of engrafted neural stem cellson the recovery of structure and function in the spinal cord transected rats.

结果 移植的神经干细胞能在宿主内存活并向前后方向迁移到脊髓内,部分神经干细胞分化为GFAP、NF 2 0 0和GAP 4 3阳性细胞。

RESULTS:In BN rat retina,Cx43 was immunolocalized to internal limiting membrane,nerve fiber layer,ganglion cell layer and pigment epithelium.Cx43 appeared in the form of distinct puncta distributed on ganglion cell.

结果:正常视网膜内界膜,视神经纤维层以及神经节细胞层明显表达,神经节细胞表达的主要位置是细胞质和细胞膜,色素上皮细胞全层表达,内外颗粒层及内外层状层无表达。

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