神经元的

In recent years, rTMS has also been used as a therapeutic tool in a variety of neuropsychiatry disorders such as Parkinson disease and depression that were conceptualized in term of a dysfuction of neuronal circuits and neurons at a cellular and molecular level.

近年来rTMS更是在治疗一系列的神经精神疾患如帕金森病、抑郁等上取得了较好的效果，但其治疗机制尚不明了，而且这些神经精神疾病又被认为是神经环路和神经元在细胞及分子水平上的功能失调，因此探讨rTMS所致的磁刺激对中枢神经系统中神经元基因活性、神经介质或/和调质、结构变化等的调控作用有着重要的意义。

The pathways that regulate tone are similar to those that regulate voluntary and involuntary motor movements and, as a final common pathway, involve the spinal reflex arc.

控制张力的传导通路和那些控制随意或非随意运动的通路是一样的，即脊髓反射弧，这是一个最后的公共通路：控制肌纤维的α运动神经元位于脊髓前角，发出反射弧的传出纤维，源自肌梭的传入冲动经由Ⅰa纤维传向脊髓，这些直接与α运动神经元连接的轴突中的一部分激活主动肌。

Results:(1)NSCs form typical neurospheres under adequate concentration in vitro, which are immunoreactive to Vimentin. Typically and terminally differentiated mature neural cells could not be found without the stimulus of mitogen or only under NSCs self-regulation and self-induction;(2)NSCs derived from hippocampus maintain the character of stem cells much longer with better biological behavior; NSCs passed to the 2-3 passage are the best to graft since they have not differentiated;(3)NSCs cultured in vitro could self-regulate and differentiate into neurospheres and progenitors positively immunoreactive to specific antibodies representing neurons, astrocytes, oligodendrocytes and Schwann cells;(4)There are widespread synaptic contacts between various kinds of descendent clones and cells;(5)Neurospheres could be formed without the stimulus of mitogen when NSCs and OECs are cocultured. Many neurospheres and cells immunoreactive to Vimentin, GFAP, MAP2, 02, p75NGFR, GFAP, S-100, Synaptosis, Vimentin, Tau (Tau is only positive in cocultureof HNSCs+HOECs) could be found;(6)The supernatant fluid triturated from adult rat spinal cord stimulates NSCs to differentiate into neurons, but do not terminally differentiate;(7)Fibroblasts and O4 oligodendrocytes are not supported to grow under this culture medium.Part II: Isolation, culture and identification of rat and human olfactory ensheathing cellsOlfactory ensheathing cells/glials are the most powerful cells to enable the regeneration of axons in the central nervous system.

结果表明：①在适宜的浓度体外培养条件下，NSCs能形成典型的神经干细胞克隆球，Vimentin免疫荧光染色阳性，单靠丝裂原刺激或NSCs自我调节和分化诱导，不会产生典型的终末分化的成熟神经细胞；②海马源性的NSCs维持干细胞特性的时间更长，生物学特性更优；③传至第2～3代的NSCs尚未分化时移植最佳；④体外培养的NSCs能自我调控分化为神经元、星形胶质细胞、O2少突胶质细胞、雪旺氏细胞染色阳性克隆球和前体细胞；⑤各种子代克隆球和细胞存在广泛的突触联系；⑥NSCs与OECs联合培养时，不需丝裂原刺激即能形成克隆球，获得大量Vimentin、GFAP、MAP2、O2、p75NGFR、GFAP、S-100、Synaptosis、Vimentin、Tau(Tau只有人HNSCs+HOECs联合培养时出现阳染)染色阳性的克隆球和细胞；⑦脊髓研磨后的上清液刺激神经干细胞向神经元方向分化，但并不出现终末分化；⑧本研究培养条件不利于成纤维细胞、O4生长。

Barrington's nucleus that located in the pontine dorsolateral tegmentum is an important site for controlling voiding in the mammalian brain and is often referred to as pontine micturition center. Barrington's nucleus sends direct projection fibers to the sacral parasympathetic nucleus in which parasympathetic preganglionic neurons innervating detrusor muscles of the bladder are distributed. Electron or chemical stimulation of Barrington's nucleus clicits bladder contraction and increases discharges of bladder postganglionic nerve. Conversely, lesion of the nucleus results in a permanent inability to empty the bladder. On the other hand, an area located just ventral to Barrington's nucleu has been reported to send projection fibers to the ventrolateral group of Onuf's nucleus in the caudal lumbar cord segments; the vetrolateral group of Onuf's nucleus is mainly composed of pudendal motor neurons innervating the external urethral sphincter muscle.

Barrington's核发出纤维投射至支配膀胱逼尿肌的骶髓副交感核，毁损该区可导致动物永久性失去排空膀胱的能力；而电刺激或化学刺激该区可引起支配膀胱的节后纤维放电增加和膀胱逼尿肌收缩。D区是紧邻Barrington's核腹侧的一团神经元，它发出纤维投射至腰骶髓Ounf核的腹外侧群，而Ounf核的腹外侧群主要是由支配尿道外括约肌的阴部神经运动神经元组成。

Moutains of evidences from laboratary and clinical data show that gene abnormality is the major etiological factor of over 40% patients affected with epilepsy.There were also 6 epilepsy genes been cloned successfully, and mutations of over 1000 genes maybe related to epilepsy. These abnormal genes influence many aspects from molecular level to the neuronal plasticity, such as the development of brain,neuronic degeneration, remodeling of neural circuits, energy metabolism, ion channel and so on.These factors would at last affect the formation of epilepsy focus, propagations of epileptic potentials, vulnerability of epilepsy cells.

大量的实验和临床资料提示基因异常是40%以上癫痫患者的病因，有6种常见全身癫痫的基因已被成功克隆，1000种以上的基因突变可能与癫痫的发作有关，这些表达异常的基因分布在脑发育、神经元变性及神经环路重组、能量代谢、离子通道等多个环节，从分子、细胞、神经元可塑性等多个方面影响着癫痫灶的形成、痫性放电的扩布及癫痫细胞的损伤，构成了癫痫的基因机制。

The results are as follows:(1) immunoreactive neurons of NPY in medulla oblongata of Taihe silky fowl, New jianghan domestic chicken are mainly localized in the inferior olivary nucleus.

结果如下：(1)在泰和乌鸡和江汉鸡的延髓中，NPY阳性神经元主要存在于下橄榄核，网状核及中缝核也少量出现；(2)在泰和乌鸡和肉鸽的小脑中，NPY阳性神经元主要存在于小脑皮质的蒲肯野氏细胞层，且以小叶顶端的蒲肯野氏细胞阳性明显，而小脑皮质的分子层、颗粒层未见阳性反应细胞；(3)泰和乌鸡延髓的网状细胞核非常发达；泰和乌鸡小脑皮质分3层，由外至内依次为分子层、蒲肯野氏细胞层和颗粒层；小脑分10叶，Ⅰ、Ⅱ和Ⅹ为单叶，其余各叶都分为2～3个小叶；(4)非洲鸵鸟迷走神经运动背核和疑核特别发达，孤束核吻侧部不发达。

Though can not accurate induce neural stem cells specific orientation differentiation yet,to accompany the unceasing deepen recognize of convection mechanisms in cells' signal convection and discovery of new cell facter,new chemo-signal, Homo sapiens would solve the question of orientation differentiation from totipotential stem cell,multipotential stem cell to neural stem cell, orientation nerver ancestor cell then special tipe neural cell, implementation functional reconstruction betwen neural cell,for ultimate solve the proble of neural regeneration developing the way.

尽管目前尚不能准确有效的诱导神经干细胞特异性定向分化为某种特定的神经元，但随着人们对细胞间信号传递机制认识的不断加深和新的细胞因子、化学信号的不断发现，人类将能够解决从全能干细胞、多能干细胞到神经干细胞、定向神经祖细胞直至特异类型神经细胞的定向分化，完成神经元之间的功能重建，为最终解决中枢神经再生这一难题而开辟道路。

These results indicate that the genome of a post-mitotic, terminally differentiated neuron can re-enter the cell cycle and be reprogrammed to a state of totipotency after nuclear transfer.

这是非同小可的，因为供体神经元只表达一个这样的蛋白，而不是完整的一套蛋白，说明大脑中所表达的嗅觉受体的多样性不是嗅觉神经元DNA中永久排列的结果。

Adhibiting the nerve cell and qurdratic capability target, by the self-adapting self-studying consistent and better abiding of the nerve cell ,the thesis realizes the conforming of the parameter not depending the canal mathematics ,furthermore the parameter can self-adjust for the information of the canal.Combining the BP nets and the routine PID controller, and adhibiting the linear optimization target function, the thesis realizes the conforming of the parameter not depending the canal mathematics, furthermore the parameter can self-adjust for the information of the canal. Using the MATLAB the thesis simulates and acquires some significative effects.

在传统PID渠道控制中引入了神经元和二次型性能指标，利用神经元自适应、自学习、并行处理及较强的容错能力，实现了PID控制中参数的整定不依赖于渠道数学模型，且PID参数能根据渠道的适时信息在线调整以满足适时控制的要求将BP网络与常规PID控制结合，并引入了最优化目标函数，利用BP网络来实现PID控制器参数的在线整定，将其运用于渠系等体积控制，并利用MATLAB软件进行了数值模拟仿真，取得了一些有意义的成果和较为满意的结果。

Alter intragastric administration of Ecr to rats, the effect of Ecr on the changes of gastric motility was detected. At the same time, the changes of frequency of electro-physiological activity, as well as expression of c-Fos in nucleus tractus solitari and dorsal-nuclei of the central bulb were also observed. The mechanism of Ecr on gastric motility were also detected by observing the activation of the NTS and DMV.

在前期工作的基础上，采用灌胃给药方法，给大鼠以最有效剂量的萝卜提取物按60mg/kg灌胃后，经胃电记录，观察其对胃运动的影响，并对其对中枢支配胃运动的迷走复合体(dorsal vagal complex， DVC)中神经元放电频率的变化以及c-Fos表达的情况为标志，观察上述两个核团中神经元被激活状况，来探讨萝卜提取物促胃动力的作用机制。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。