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Isolation, cultivation and identification of human embryonic NSC from cerebral cortex of 7-12 week abortus. By using flow cytometry and MTT assay, the effects of various concentration of TSPG and TSPG cooperating with cytokines in NSC culture media for 3 days on proliferation of human embryonic NSC has studied. By employing immunocytochemistry assay of the expression of tyrosine hydroxylase, the effect of different dilution of TSPG and TSPG cooperating with IL-1 on induced differentiation of human embryonic NSC into dopaminergic neuron has researched.

从7~12周人工流产胚胎脑组织中分离培养人胚胎NSC,免疫细胞化学鉴定NSC特异性nestin抗原表达,在NSC的培养体系中加入TSPG,采用流式细胞术和MTT法检测不同浓度TSPG以及TSPG与EGF,bFGF联合应用对人胚胎NSC增殖的影响;经免疫细胞化学检测培养细胞酪氨酸羟化酶表达,分析不同浓度TSPG以及TSPG与IL-1联合应用对NSC定向诱导为DA能神经元的影响。

The primary noxious afferent neurotransmitters excitatory glutamate and substance P play a pivotal role in the development and maintenance of chronic inflammatory pain.

初级伤害性传入神经元的兴奋性递质谷氨酸和P物质对慢性炎性痛的形成和维持起到非常关键的作用。

In summary, the activation of Lateral amygdaloid nucleus can directly effect the function of auditory neurons in A-Ⅰ, and participate in integration and modulation of auditory information at cortex level. This influence holds the character of the quantity-effect physiological relevance and cumulating effect.

以上实验结果表明,作为边缘系统重要的结构,杏仁外侧核可以直接影响听皮层AⅠ区神经元的功能活动,在皮层水平参与对听觉信息的整合和加工处理。

At this stage, the brain activity of the neurons and clear the same time.

在这个阶段,大脑的神经元的活动与清醒的时候相同。

In summary, age-related changes of GABA metabolism and dendrite on rat visual cortex were observed in the present study.

大鼠初级视皮层神经元的GABA分布、代谢具有明显的年龄相关性的变化。

In addition to glial cells, CNTF immunoreactivity are widely localized at neurons in the olfactory bulb, cortex, hippocampus, cerebellum, diencephalon and brainstem.

结果表明,CNTF免疫反应物质除广泛存在于中枢神经系统的胶质细胞外,亦有神经元的广泛染色,而且分布广泛,在嗅脑、大脑皮层、海马、小脑、间脑、脑干中均有明显的染色。

Immunocytochemical studies showed that the transduced muORs were expressed in all types of DRG neurons.

免疫细胞化学研究表明转导的 muORs在背根神经节神经元的各种类型的细胞中都能表达。

Results Under optical lens positive products of ACP reaction were brown particles scattered in cyton and process;under electron lens its positive products were black precipitates with high electron density scattered in lysosome and Golgi bodies in different shapes,and often companied by linear lysosome and some cell organs.

结果光镜下可见 ACP 反应阳性产物为棕色颗粒,散在分布于神经元的胞体及突起内;电镜可见其阳性产物为电子密度高的黑色沉淀,分布于不同形状的溶酶体及高尔基体内,并常见线状溶酶体与一些细胞器相伴行。

The effect of bFGF on development and survival of basal forebrain NOS-positive neurons.

bFGF对细胞培养和移植的基底前脑神经元的发育和生长的作用。

OBJECTIVE: To investigate the association between olfactory disturbance and ultrastructural mitochondrial changes in olfactory bulb neurons of aged rats in comparison with young rats.

目的:对衰老和年轻大鼠嗅球内神经元的线粒体超微结构进行对比观察,分析衰老大鼠嗅觉障碍与嗅球内神经细胞线粒体改变的关系。

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