神经元的

These results suggest that the activate spleen-energy method can achieve appetitive purpose by not only affecting the gastrointestinal tract function in periphery, but also regulating the process of ingestion in brain. The basic research about ingestion indicate: one hand, after food enters the gastro-intestinal tract, the endocrine cell of the intestinal tract is activated and secrete many kinds of brains intestines peptide, the level of the brain intestines peptide in blood as the periphery signal spreads to the central nervous system regions that control digest and feeding behavior. On the other hand, it has been shown that dorsal parabranchial neurons, containing CCK-8S, extend fibers to the VMH and are involved in the inhibition of feeding .

关于摄食控制的基础研究表明，一方面食物进入胃肠道后，激活肠道的内分泌细胞，分泌多种脑肠肽如CCK-8S，这些脑肠肽在血中的水平作为外周信号可通过"肠—脑轴"传入中枢神经系统介导消化和摄食行为的部位，影响摄食中枢神经元的活动调节动物的摄食行为；另一方面中枢神经元可合成释放内源性的神经肽直接作用于摄食中枢神经元调节摄食，如中枢鳃旁体神经元的神经纤维就可延伸到达VMH，并且合成释放内源性的CCK-8S。

4DRG was co-culture with sciatic nerve segment in 10鸖 DMEM;the axons were longer and surround the sciatic nerve segment which was regard as anew evidence for chemotropism.

结果：(1)在无血清条件下单独培养的DRG，背根神经节的轴突数目众多，外形纤细弯曲，不成束，并且施万细胞和成纤维细胞稀少，所以可以排除两者对轴突生长的影响，为观察来源于变性坐骨神经段的可溶性因子对轴突生长的作用提供了有利条件；(2)在无血清条件下DRG和变性坐骨神经段联合培养，①先单独培养DRG，4天后待神经元轴突长出，再与坐骨神经段联合培养，观察到神经元的轴突数目减少，外形挺直，部分轴突之间相互粘附成束；②变性坐骨神经段和DRG同时联合培养，神经元的轴突数目明显减少，外形粗壮，轴突之间相互粘附成束；(3)有血清条件下单独培养DRG，轴突数目较多，外形挺直，长短不一，部分神经元的轴突之间相互粘附，施万细胞和成纤维细胞数目众多，观察到的轴突生长情况受到施万细胞和成纤维细胞的直接或者间接的影响。

It is suggested that there are some Glu sensitive neurons in sublayer Ⅱaf of pigeon optic tectum,the most of which show excitation to exogeous Glu.These data observed in slices provide a strong evidence that Glu may act as a neurotransmitter involving in the activity of the sublayer Ⅱaf neurons in pigeon optic tectum.

以上结果提示，家鸽顶盖Ⅱaf亚层内存在Glu敏感神经元，外源性的Glu对这些神经元有兴奋作用，Glu可能作为神经递质参与了家鸽顶盖Ⅱaf亚层神经元的活动。

The extremities were titanic, and there was no response to touch stimulations.Our results suggested that Pentobarbital sodium inhibited neuronal firing through GABA_A receptors, therefore the local field potentials and neural firing rates were inhibited; Urethane acted on many kinds of receptors including inhibitory and excitory ones, so the local field potentials did not change significantly; however, because of the multiform receptors on the neurons, the changing of firing rates to different neurons were not the same; Ketamine mainly acted on the NMDA receptors, therefore the local field potentials and neural firing rates were enhanced, and most neural firing rates would be increased.

从这三种麻醉剂的底层作用机制来看，戊巴比妥钠通过作用于GABAA受体对神经细胞发挥抑制性作用，因此表现为场电位和神经元放电的降低；氨基甲酸乙酯同时作用于多种受体通道，既有兴奋性受体，也有抑制性受体，因此，对场电位的影响不大，但对单个神经元来说，每个神经细胞上的受体通道分布差异较大，因此，单个神经元的放电频率变化会有所不同；盐酸氯胺酮主要作用于兴奋性的NMDA受体，故整体场电位活性增加，多数神经元的放电频率也会有所升高。

These results suggest that the activate spleen-energy method can achieve appetitive purpose by not only affecting the gastrointestinal tract function in periphery, but also regulating the process of ingestion in brain. The basic research about ingestion indicate: one hand, after food enters the gastro-intestinal tract, the endocrine cell of the intestinal tract is activated and secrete many kinds of brains intestines peptide, the level of the brain intestines peptide in blood as the periphery signal spreads to the central nervous system regions that control digest and feeding behavior. On the other hand, it has been shown that dorsal parabranchial neurons, containing CCK-8S, extend fibers to the VMH and are involved in the inhibition of feeding .

关于摄食控制的基础研究表明，一方面食物进入胃肠道后，激活肠道的内分泌细胞，分泌多种脑肠肽如CCK-8S，这些脑肠肽在血中的水平作为外周信号可通过&肠—脑轴&传入中枢神经系统介导消化和摄食行为的部位，影响摄食中枢神经元的活动调节动物的摄食行为；另一方面中枢神经元可合成释放内源性的神经肽直接作用于摄食中枢神经元调节摄食，如中枢鳃旁体神经元的神经纤维就可延伸到达VMH，并且合成释放内源性的CCK-8S。

These results can lead to the following conclusion:〤GRP synthesized by the motoneurons may be a self-serving neurotrophic factor after axotomy.(2) CGRP may serve as a "injury signal" activating both the central and peripherical gli

本研究结果提示，运动神经轴突损伤后，运动神经元合成分泌的CGRP对神经元自身具有直接的神经营养作用，CGRP还可以作为损伤神经元分泌的&损伤信号&激活中枢和外周胶质细胞，从而为运动神经元的存活和轴突再生创造有利的微环境。

It has been proved by previous studies that peripheral nerve injury can induce a series of changes of expression of some neural active material and their receptors either in the somata or in the terminals of the damaged primary afferent neurons and motoneurons. These changes may reflect a re-ordering of metabolic priorities so that the synthesis of trophic factors or growth-associated proteins augmented while that of neurotransmitter-related proteins decreased.

以往的研究表明，周围神经损伤能够引起受损神经元(包括初级传入神经元和运动神经元)的胞体或终末内某些神经活性物质及其受体的表达发生一系列变化，其中与神经生长和营养有关的物质常发生上调，而与神经传递有关的物质则发生下调；与此同时，受损神经元的周围常伴随发生胶质反应及神经胶质增生等变化。

These results suggest that in the central nervous system of small mammals, AP waveforms mature rapidly in many types of neurons, thus, coordinated activities in neuronal circuits may occur. In early postnatal development, GABA receptor mainly mediates an excitation effect, depolarizes P6 MGBv neurons and dramatically increases the frequency of AP firing. Inhibition of GABA receptor matures slowly along with increment of postnatal days.

结论大鼠MGBv神经元的AP波形成熟较快，可在中枢神经系统的神经元环路中产生协调性的活动；在MGBv神经元发育早期，GABA主要作为兴奋性神经递质而发挥作用，它可使P6的MGBv神经元产生去极化，AP发放频率显著增加，随着出生后日龄的增加，GABA受体的抑制作用才逐渐发育成熟。

GnRH 1R neurons were found as early as on the first day of culture. During the 1st-3rd days in vitro, they grew fastest. The average growth rates of the perikarya and processes were 45 59μm 2/d and 19 39μm/d, respectively.

培养1d即可见GnRH－IR神经元；1～3d，GnRH－IR神经元生长最快，胞体和突起的平均生长速度分别为45.59μm2／d和19.39μm／d，染色加深；培养7d，GnRH－IR神经元的胞体截面积、胞体染色强度和突起长度均达最高峰，分别为171.21±43.17μm2，0.289±0.034，87.01±22.33μm；培养14d，GnRH－IR神经元的胞体大小和突起长度维持在7d时的水平，但染色减弱。

The other seven neurones were tested by the stimulation of SPN with the current intensity of 100, 200, 300, 400 and 500μA respectively.

同样刺激DPN对RVLM神经元的作用也随强度的改变而改变，在7只神经元也分别用100、200、300、400和500μA的电流刺激DPN，观察对其影响；只有强度增至500μA时才使所有神经元兴奋，且引起兴奋的潜伏期差别很大；个别神经元诱发放电的潜伏期也随强度改变。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。