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The effective stimulating sites were indentified by defensive cardiovascular response and other components of defence reaction evoked by 3 s trains of rectangular cathodal pulses (100Hz, 0.5ms pulse duration, 70 -150μA intensity for dPAG and 150-250μA for PF) delivered from a programmed multichannel constant current stimulator. The responses included an increase in blood pressure and heart rate, pupillodilation and an increase in the rate and depth of respiration. Currents of 1Hz, intensity of 100- 150μA for dPAG and 200-250μA for PF were used to observe the effect of the stimulation of them on RVLM neurones. The deep peroneal nerve and superfacial deep peroneal nerve were carefully separated, and put on a pair of stimulating electrode respectively, Both nerves were stimulated with constant rectangular pulses of 1Hz, 0.5ms duration and 300μA current intensity to see their effects on RVLM neurones. The baro-and chemo-receptors were activated by the intravenous injection of phenylephrine or 0.208 M NaH2PO4. The degree of convergent inputs to individual RVLM neurone was studied by the stimulation of the sites mentioned above. It was found that neurones about 61.4%(27/44) and 35.3%(6/17) received convergent inputs from three and four places respectively.

过去的研究主要集中于整体水平,利用电刺激或化学刺激RVLM神经元,观察对血压、心率及交感活动的影响,并对影响心血管交感活动的神经递质进行了广泛探讨,积累了大量资料;在神经元水平,主要研究刺激脑内核团、外周神经、化学感受器及压力感受器等对RVLM神经元放电的影响和某些神经递质影响神经元活动的机制、然而,对RVLM神经元如何整合不同信息、其可能突触机制等研究得较少,本互作拟采用在体细胞内、细胞外记录电活动与微电泳,结合中枢核团及外周神经电刺激等方法,对RVLM神经元在不同状态下的整合作用规律与可能机制进行初步探讨,为进一步了解这一复杂的整合过程提供新的理论依据。

Methods Unit responses were recorded extracellularly from thalamic ventroposterolateral nucleus in anesthetized rats.

采用在体细胞外单位放电记录技术,记录麻醉状态下大鼠丘脑腹后外侧核触觉神经元的放电活动。

In this paper, we introduced the method and set of cat perceptual learning and research the character of orientation discrimination and contrast detection of cat, and we also research the effect of visual perceptual learning on the response of the Dorsal Lateral Geniculate Nucleus neuron with the method of single-neuron in vivo recording techniques, by which to the ends of knowing the mechanism of neuron in the perceptual learning. Perceptual learning in orientation discrimination and contrast detection was first observed by psychophysics method in cat in the following two experiments.

本研究介绍猫的视觉学习实验装置和训练方法,观察了方位辨别和对比度检测两种知觉学习的特点,并结合在体细胞外单细胞记录技术研究了视知觉学习对外膝体神经元反应特性的影响,初步探讨猫视知觉学习的神经机制。1、运用心理物理学方法进行了两个知觉学习实验,分别观察了猫对正弦光栅方位辨别和对比度检测的知觉学习。

To clarify the effect of lidocaine oncholinergic synapse, we reconstructed a cultured soma–somachemical synapse model consisting of two identified visceraldorsal 4 (VD4) and left pedal e-1 (LPeD1) neurons from the snail, Lymnaea stagnalis , in vitro , and used it to determine how lidocaineaffects cholinergic synaptic transmission.

为了阐明利多卡因对胆碱能突触的影响,我们从椎实螺属 stagnalis 的蜗牛离体重建了含两种已被鉴定的内脏背4( VD4)和左足 e-1( LPeD1)神经元的培养体细胞-体细胞化学突触模型,来研究利多卡因如何影响胆碱能突触传递。

Methods The effect of mianserin (2 mg/kg, i. v.) on the spontaneous firing of subthalamic neurons was observed by extracellular recording in vivo.

应用在体细胞外电生理学记录的方法观察经静脉注射的米安色林(2 mg/kg)对大鼠STN神经元自发电活动的作用。

We show gain of function of excitatory neurotransmission due to increased action-potential-evoked Ca(2+) influx and increased probability of glutamate release at pyramidal cell synapses but unaltered inhibitory neurotransmission at fast-spiking interneuron synapses.

我们发现兴奋性神经传递的功能增益来自于诱发Ca(2+)内流动作电位的增加,并可能与谷氨酸盐在椎体细胞突触中的释放增加有关,但与快速成峰中间神经元突触的抑制性神经传递无关。

Extracellularly recording the responses of dLGN neurons to sinusoidal gratings with different orientations showed that optimum orientations of dLGN neurons in trained cats, compared with that in untrained cats, did not shift in favour of the trained orientation. For cells with receptive fields located within 15°visual acuity away from the foveae, there are no difference in orientation bias and firing rate at trained orientation between the trained cats and untrained cats.

细胞外记录外膝体背核神经元对不同方位正弦光栅刺激的反应显示,垂直或水平方位正弦光栅信号训练的猫,与正常猫相比,其外膝体细胞的最优方位并未向着训练方位发生明显改变,对于感受野位于中央区15 度视角以内的细胞来说,其方位选择性强度以及在训练方位的发放强度与正常猫无明显差异。

In vitro coculture of NSC and PAS in a ratio of 2:1 may be more advantageous for NSC in differentiating into neurons.

在离体细胞共培养的实验中,因细胞的比例不同,NSC分化亦存在差异,其中NSC:PAS为2:1时,NSC分化为神经元的比例最高。

The aim of this study is to investigate the influence of the lateral nucleus of amygdala stimulation on the auditory response in the auditory cortex evoked by frequency-modulated sound.

采用在体细胞外单细胞记录方法,研究电刺激杏仁外侧核对调频声所诱发的听皮层神经元反应的影响。

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