神经元

Results showed:(1) The number of ChAc-IR neurons (62. 25±1. 45) in the group of visceral pain increased obviously. The positive neurons (46. 38±1. 27) in the group of electroacupuncture and visceral pain were lower than those of VP (p.05). These indicate that there were changes in cholinergic neurons' activity, increasing of ChAc function and turbulence of intestinal motility in rat's ileum following the acute colonitis.

结果发现：（1）内脏痛组ChAc-IR神经元（62.25±1.45）明显增高，电针+内脏痛组阳性神经元（46.38±1.27）明显低于内脏痛组，二组之间存在显著差异（p.05），提示急性结肠炎可致继发性回肠内在胆碱能神经元活动的改变，ChAc活性增高，肠动力紊乱，而电针可抑制这一改变。

At first, a model of a pair of excitable neurons coupled via gap junction is built by Chay model, the effect of the strength of gap junction on the synchronous oscillation is studied;then, the synchronous oscillations of a 2-D network are also studied;at last, the Lyapunov exponent and phase portrait are utilized in the analysis of nonlinear, the approximate entropy is utilized to measure the complexity.

首先以Chay模型为基础建立了两个神经元电突触耦合模型，研究了不同电突触耦合强度对两个神经元同步振荡的影响；其次，研究了可兴奋神经元组成的二维网络的同步振荡；最后，应用李雅普诺夫指数和相图分析了同步振荡的非线性，应用近似熵分析了同步振荡的复杂性。

The remaining small part protruded with several crassitude processes which were MAG positive. Ratio of neuron is different(30.2+3.8)%(50.9+2.8)%(58.6+3.3)% and (66.7+4.1)%(P.01). Conclusions is Sodium nitroprusside can inhibit the proliferation and promote the differentiation of spinal neural stem cells into neuronal but not glial cells in vitro.

相差显微镜下大部分神经干细胞伸出多个细长突起，且MAPZ免疫荧光染色增强：部分为有多个短小突起、GFAP阳性表达；部分细胞为少数粗大突起，MAG染色阳性。4组神经元数与三种细胞总数之比，即神经元的比例分别为(30.2士3.8)%、(50.9士2.8)%、(58.6士3.3)%和(66.7士4.1)%，可见硝普钠浓度越高，神经元比例越大(只0.01)。

Immunoprecipitation and cresyl fast violet staining were employed to detect the expression of relevant message proteins, activation and the death of the hippocampal neurons.results it was found that hpk1 was expressed in rat hippocampus neurons.conclusions administration of hpk1 antisense oligodeoxynucleotides can significantly protect hippocampus neurons from apoptosis without dose-dependence.

使用免疫沉淀和焦油紫染色法等技术检测相关信号蛋白的表达、活化水平及神经元细胞的死亡。结果在大鼠海马神经元中有hpk1的表达，脑室注射as-odns大鼠海马ca1区存活锥体细胞明显增多。结论 hpk1反义寡核苷酸对缺血/再灌注引起的海马神经元凋亡具有保护作用。

This paper used Elman neural network to establish the model of irrigation water use prediction, having four importing units and exporting units. The determination of concealed level neural single number used the cut-and-try method, ten units were used.

运用神经网络中Elman反馈型神经网络建立了灌溉用水量预测模型，模型输入层神经元数目为4，输出层神经元数目为1，隐含层神经元数目确定采用试验法，最终确定为10。

Some SPR-LI neurons were also positive for TH. After administration of vasopressin, SPR-LI structures became denser, especially at levels of pyramidal decussation and area postrema . The dendrites of motor dorsal nucleus of X in the dorsal part of MVZ appeared thin and straight in morphology instead of curl and thick outlooks. These results implicate that some SPR-LI neurons might receive or regulate the cardiovascular stress information induced by vasopressin.③In PHA-L/FOS/TH triple-labeled experiments, seven kinds of immunohistochemical structures were found in MVZ. They were FOS-LI neurons, TH-LI neurons, PHA-L positive fibers, PHA-L positive fibers terminated on FOS-LI or TH-LI neurons, FOS/TH doublelabeled neurons, PHA-L positive fibers terminated on FOS/TH double-labeled neurons.

在锥体交叉和最后区水平，SPR免疫反应阳性结构较正常组明显增强；背内侧部迷走神经运动背核的SPR纤维也由弯曲的粗纤维变为细的直纤维，30％的SPR阳性神经元为SPR/FOS双标记神经元，提示这些神经元参与感受心血管伤害性刺激信息，继而参与心血管活动信息的中枢传导或整合；③三重标记研究结果显示，在MVZ可见到七种免疫反应阳性结构，即PHA-L，FOS，TH，FOS/TH，PHA-L/FOS，PHA-L/TH，PHA-L/FOS/TH。

The part of the ectoderm in a vertebrate embryo that lies on either side of the neural tube and develops into the cranial, spinal, and autonomic ganglia.

神经脊，脊椎动物胚胎外胚层的一部分。位于神经管的两侧，并逐步发展成头颅神经元、脊髓神经元、自主神经系统神经元

Three RNAi target sites (named as Abil1, Abil2, Abil3) targeting the E3B1 (NCBI: NM-024397), an identified target sites and positive control GAPDH-A were selected. The pGenesil-1 eukaryotic expression vectors with the neoR mark and GFP green fluorescent mark were selected to construct E3B1 RNAi plasmid. The effect of RNAi targeting various sites on E3B1 genes expression was evaluated by testing the transfection efficiency with fluorescence microscope and western blot. The most effective siRNA in inhibiting E3B1 genes were screened. The most effective siRNA in inhibiting E3B1 genes screened and used to transfect the neuronal cells. After that, the inhibitor of axon growth was added and the axon growth was observed. Result: The siRNA expressing plasmids targeting the E3B1 were constructed successfully.

选择针对E3B1(NCBI： NM-024397)RNAi的3个靶位点Abil1、Abil2、Abil3和1个不针对任何mRNA的RNAi靶位点以及甘油醛－3-磷酸脱氢酶，用带有neoR选择标志和GFP绿色荧光标志的真核表达载体pGenesil-1构建E3B1 RNAi质粒，分别转染培养的神经元，在荧光显微镜下观测转染率，经G418筛选得到单一的转染细胞，并用Western blot法检测各转染组神经元E3B1蛋白的表达情况，选出具有最佳抑制效应的siRNA转染神经元，加入轴突生长抑制物，观测轴突生长情况。

The expressions of iNOS mRNA and proteins in VTA and FrA increased less than those in CPu and SNpc.

表达增高的iNOS可能对SNpc和VTA脑区DA能神经元及CPu和FrA脑区的DA能神经元末梢起了损伤作用，从而导致了DA能神经元的减少及其末梢退变的发生。

Results from whole-cell patch clamp showed: 1 BmK Ⅰ(α-like neurotoxin) was found to prolong the duration of action potential and promote the amplitude of action potential. The effects could be attributed to the selective action of BmK Ⅰ on the TTX-S Na〓 channel: slowing the inactivation process of Na〓 channel, increasing the amplitude of peak Na〓 currents; 2 BmK abT, a new-type neurotoxin with higher sequence homology to β-type scorpion neurotoxin, was found to slow the inactivation process of Na〓 channel and prolong the duration of action potential in DRG neurons.

全细胞膜片钳研究发现：1）类α型哺乳动物毒素BmK Ⅰ可延长部分DRG神经元全细胞动作电位的时程，增加动作电位的幅度，这些效应可归咎于BmK Ⅰ选择性地作用于DRG神经元TTX—S钠通道，延缓TTX—S钠电流的失活化相，峰钠电流幅度增加；2）BmK abT，一个与β型毒素同源性较高的新型蝎毒素可抑制DRG神经元全细胞钠电流失活化过程，使动作电位时程增加。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了