神经元

The result of framework protein and lysosome marked with fluorescence was observed in sym-acorched laser scaning microscope: red fluorescent β-tubulin and green fluorescent eathepsin in nerve cell were seen in distribution in the same sample. The distribution of both was the same, and was located in around nucleus in the early of differentiation, and in cytoplasm and apophysis in the maturity of differentiation.

共焦激光扫描显微镜镜观察荧光双标的骨架蛋白和溶酶体的染色结果：在同一标本上，不同波长的单光激发可分别见到发红色荧光的β-tubulin和发绿色荧光的组织蛋白酶D在神经元内的存在及分布情况，二者的分布区域大致相同，神经元分化初期，较集中分布在核周附近，神经元分化成熟则分布于胞质及突起内。

In the present study we transfected F-GFP into cultured hippocampal neurons at 5 days in vitro(DIV 5).Then ASICs antagonists were applied to the neuronal medium to inhibit the function of ASICs.Dendritic growth and arborization of cultured hippoeampal neuron were observed in DIV8 and DIV14. We attempted to determine whether and how ASICs regulate dendritic development.

在本研究中，我们在体外培养第五天(days in vitro，DIV5)的原代海马神经元中转染定位在膜上的GFP，随后加入ASICs拮抗剂抑制ASICs的功能，观察DIV8和DIV14这两个时间点海马神经元的树突生长、分支复杂程度，来研究酸敏感离子通道是否会影响海马神经元的树突发育。

Research concerning research status and development of CNTF was selected, including changes of CNTF following nerve injury; the repair mechanism and protection of CNTF on neurons; regulation on reparative process; the recovery of axoplasmic transport function of axoplasm, as well as the regeneration on injured nerves.

纳入与神经损伤与睫状神经营养因子研究现状与发展密切相关的研究，包括神经元损伤后的变化；睫状神经营养因子对神经元的修复机制及保护作用，对修复过程的调控，对神经元轴浆运输功能的恢复，对损伤神经的再生作用。

The lesion, the changes of activity of cholinesterase and acid phosphatase of motor neurons in lateral nucleus of spinal cord anterior horn, the function of axoplasm transportation and nerve conduction, the regeneration of axons and myelin sheath, and the recovery of sciatic nerve function were examined at 7, 14, 30 and 90 days intervals after operation, using Nissl and enzyme histochemistry staining, electrophysiological technique, hydrogen-peroxide oxidoreductase retrograde trace method, axon image analysis, and measurement of sciatic function index. The spinal cord anterior horn of injury side was compared to the correspondence region of spinal cord.

分别于术后7、14、30和90d应用酶组织化学方法、电生理方法、HRP （hydrogen-peroxide oxidoreductase）逆行示踪方法、轴突图象分析方法以及坐骨神经功能指数（sciatic nerve function index，SFI）测量等方法检测坐骨神经损伤后对应脊髓神经元的存活率、神经元胞体酶系变化、损伤神经在轴浆运输、电传导以及轴突、髓鞘再生等方面的恢复情况，探索外周神经损伤后OECs及几丁质对神经元的保护作用以及对新生神经功能恢复的作用，为外周神经损伤的治疗提供新的理论基础。

Using in vivo intracellular recording technique, we studied the binocularity of the dorsal lateral geniculate nucleus neurons in the monocular eyelid sutured cats. We found that almost all the neurons responded to the flashing spots to the nondominant eyes, and there was no significant difference of the response properties between the neurons in the deprived and non-deprived layers.

采用在位胞内记录技术，研究了单眼视觉剥夺猫外膝体神经元的双眼反应特性，结果发现单眼剥夺猫外膝体几乎所有的双眼反应神经元都对非优势眼的闪烁光斑刺激有不同程度的反应，并且剥夺层和非剥夺层神经元在反应特性上没有明显差异。

ChAc is an Ach's biological synthesizing enzyme and special mark of every cholinergic neuron.

ChAc是乙酰胆碱的生物合成酶，也是胆碱能神经元的特异标志酶，存在于所有胆碱能神经元，ChAc免疫反应活性被用于鉴别中枢和外周的胆碱能神经元。

The effects and mechanism of GABAergic neurons, NOergic neurons, opioid peptide and cyclic adenosine monophosphate in the nucleus reticularis thalami on sleep-wakefulness cycle of rats and the effects and mechanism of the 5-HTergic nerve fibers project from the nucleus raphes dorsalis to RT on sleep-wakefulness cycle of rats were investigated with the methods of brain stereotaxic, nucleus spile, microinjection and polysomngraphy.1. The effects of GABAergic neurons in RT on sleep-wakefulness cycle of rats1.1 Microinjection of 3-mercaptopropionic acid (3-MP, a kind of glutamate decarboxylase inhibitor) into RT. On the day of microinjection, sleep only decreased a litter. On the second day, sleep marked decreased and wakefulness marked increased. On the third and fourth day, sleep and wakefulness stages resumed to normal.1.2 Microinjection of gamma-amino butyric acid (GABA 1.0μg) into RT enhanced sleep and reduced wakefulness compared with control; while microinjection of L-glutamate (L-Glu, 0.2μg) decreased sleep and increased wakefulness; microinjection of bicuculline (BIC, 1.0μg), a GABAA receptor antagonist, enhanced wakefulness and reduced sleep; microinjection of baclofen (BAC, 1.0μg), GABAB receptor agonist, had the same effects as GABA.2. The effects of NOergic neurons in RT on sleep-wakefulness cycle of rats2.1 Microinjection of L-arginine (L-Arg, 0.5μg) into RT decreased sleep compared with control, but there were on statistaical difference between L-Arg group and control; while microinjection of sodium nitroprusside (SNP, 0.2μg), a NO donor into RT, sleep marked decreased and wakefulness marked increased. Microinjection of nitric oxide synthase inhibitor, N-nitro-L-arginine (L-NNA, 2.0μg) into RT enhanced sleep and reduced wakefulness.2.2 After simultaneous microinjection of L-NNA (2.0μg) and SNP (0.2μg) into RT, SNP abolished the sleep-promoting effect of L-NNA compared with L-NNA group; after simultaneous microinjection of L-NNA (2.0μg) and L-Arg(0.5μg) into RT, we found that L-NNA could not blocked the wakefulness-promoting effect of L-Arg.3. The effects of opioid peptide in RT on sleep-wakefulness cycle of rats3.1 Microinjection of morphine sulfate (MOR, 1.0μg) into RT increased wakefulness and decreased sleep compared with control; while microinjection of naloxone hydrochloride (NAL, 1.0μg), the antagonist of opiate receptors, into RT, enhanced sleep and reduced wakefulness.3.2 After simultaneous microinjection of MOR (1.0μg) and NAL (1.0μg) into RT, the wakefulness-promoting effect of MOR and the sleep-promoting effect of NAL were not observed compared with control.4. The effects of cAMP in RT on sleep-wakefulness cycle of rats Microinjection of cAMP (1.0μg) into RT increased sleep and decreased wakefulness compared with control; microinjection of methylene blue (MB,1.0μg) into RT enhanced sleep and reduced wakefulness compared with control.5. The effects of the 5-HTergic nerve fibers project from DRN to RT on sleep-wakefulness cycle of rats5.1 When L-Glu (0.2μg) was microinjected into DRN and normal sodium (NS,1.0μg) was microinjected into bilateral RT. We found that sleep was decreased and wakefulness was increased compared with control; when L-Glu (0.2μg) was microinjected into DRN and methysergide (MS,1.0μg), a non-selective 5-HT antagonist, was microinjected into bilateral RT, We found that sleep was enhanced and wakefulness was reduced compared with L-Glu group.5.2 When p-chlorophenylalanine (PCPA, 10μg) was microinjected into DRN and NS (1.0μg) was microinjected into bilateral RT, We found that sleep was increased and wakefulness was decreased compared with control; microinjection of 5-hydroxytryptaphan (5-HTP, 1.0μg), which can convert to 5-HT by the enzyme tryptophane hydroxylase and enhance 5-HT into bilateral RT, could block the effect of microinjection of PCPA into DRN on sleep-wakefulness cycle.

本研究采用脑立体定位、核团插管、微量注射、多导睡眠描记等方法，研究丘脑网状核(nucleus reticularis thalami，RT)中γ-氨基丁酸(gamma-amino butyric acid ，GABA)能神经元、一氧化氮(nitrogen monoxidum，NO)能神经元、阿片肽类神经递质、环一磷酸腺苷(cyclic adenosine monophosphate，cAMP)及中缝背核(nucleus raphes dorsalis，DRN)至RT的5-羟色胺(5-hydroxytryptamine，5-HT)能神经纤维投射对大鼠睡眠-觉醒周期的影响及其作用机制。1 RT内GABA能神经元对大鼠睡眠-觉醒周期的影响1.1大鼠RT内微量注射GABA合成关键酶抑制剂3-巯基丙酸(3-MP，5μg)，注射当天睡眠时间略有减少，第二日睡眠时间显著减少，觉醒时间明显增多，第三、四日睡眠和觉醒时间逐渐恢复至正常。1.2大鼠RT内微量注射GABA受体激动剂GABA( 1.0μg)后，与生理盐水组比较，睡眠时间增加，觉醒时间减少；而RT内微量注射L-谷氨酸(glutamic acid， L-Glu， 0.2μg)后，睡眠时间减少，觉醒时间增加；RT内微量注射GABAA受体阻断剂荷包牡丹碱(bicuculline，BIC，1.0μg)后，睡眠时间减少，觉醒时间增加；RT内微量注射GABAB受体激动剂氯苯氨丁酸(baclofen，BAC，1.0μg)后，产生了与GABA相似的促睡眠效果。2 RT内NO能神经元对大鼠睡眠-觉醒周期的影响2.1大鼠RT内微量注射NO的前体L-精氨酸(L-Arg，0.5μg)后，与生理盐水组对比，睡眠时间略有减少，但无显著性意义；而RT内微量注射NO的供体硝普钠(Sodium Nitroprusside，SNP，0.2μg)后可明显增加觉醒时间，缩短睡眠时间；微量注射一氧化氮合酶抑制剂L-硝基精氨酸(L-arginine，L-NNA，2.0μg)后，引起睡眠时间增多，觉醒时间减少。2.2大鼠RT内同时微量注射L-NNA(2.0μg)和SNP(0.2μg)后与L-NNA组比较发现SNP逆转了L-NNA的促睡眠作用；RT内同时微量注射L-NNA(2.0μg)和L-Arg(0.5μg)后，与L-NNA(2.0μg)组比较发现L-Arg可以增加觉醒而缩短睡眠，其促觉醒作用未能被NOS的抑制剂L-NNA所逆转。3 RT内阿片肽对大鼠睡眠-觉醒周期的影响3.1大鼠RT内微量注射硫酸吗啡(morphine sulfate，MOR，1.0μg)后与生理盐水组对比，睡眠时间减少而觉醒时间增加； RT内微量注射阿片肽受体拮抗剂盐酸纳洛酮(naloxone hydrochloride，NAL，1.0μg)后与生理盐水组比较，睡眠时间增加而觉醒时间减少。3.2大鼠RT内同时微量注射MOR(1.0μg)和NAL(1.0μg)后，与生理盐水组对比，原有的MOR促觉醒效果和NAL的促睡眠效果都没有表现。4 RT内环一磷酸腺苷信使对大鼠睡眠-觉醒周期的影响大鼠RT内微量注射cAMP(1.0μg)后与NS(1.0μg)组比较，睡眠时间增多而觉醒时间减少；RT内微量注射亚甲蓝(methylene blue，MB，1.0μg)后，与NS组比较，睡眠时间增多而觉醒时间减少。5中缝背核投射到丘脑网状核的5-羟色胺能神经纤维对大鼠睡眠-觉醒周期的影响5.1大鼠DRN内微量注射L-Glu(0.2μg)，同时在双侧RT内微量注射NS (1.0μg)后，与对照组(DRN和双侧RT注射NS， 0.2μg)比较，睡眠时间减少，觉醒时间增多；大鼠DRN内微量注射L-Glu(0.2μg)，同时在双侧RT内微量注射二甲基麦角新碱(methysergide， MS， 1.0μg )后，与对照组(DRN注射L-Glu 0.2μg，双侧RT注射NS 1.0μg)比较，睡眠时间增多，觉醒时间减少。5.2大鼠DRN内微量注射对氯苯丙氨酸(p-chlorophenylalanine，PCPA，10μg)，同时在双侧RT内微量注射NS (1.0μg)后，与对照组(DRN和双侧RT注射NS， 1.0μg)比较，睡眠时间增多，觉醒时间减少；大鼠DRN内微量注射PCPA(10μg)，产生睡眠增多效应后，在双侧RT内微量注射5-羟色胺酸(5-hydroxytryptaphan ， 5-HTP， 1.0μg )后，与对照组(DRN注射PCPA 10μg，双侧RT注射NS 1.0μg)比较，睡眠时间减少，觉醒时间增多。

GnRH 1R neurons were found as early as on the first day of culture. During the 1st-3rd days in vitro, they grew fastest. The average growth rates of the perikarya and processes were 45 59μm 2/d and 19 39μm/d, respectively.

培养1d即可见GnRH－IR神经元；1～3d，GnRH－IR神经元生长最快，胞体和突起的平均生长速度分别为45.59μm2／d和19.39μm／d，染色加深；培养7d，GnRH－IR神经元的胞体截面积、胞体染色强度和突起长度均达最高峰，分别为171.21±43.17μm2，0.289±0.034，87.01±22.33μm；培养14d，GnRH－IR神经元的胞体大小和突起长度维持在7d时的水平，但染色减弱。

The other seven neurones were tested by the stimulation of SPN with the current intensity of 100, 200, 300, 400 and 500μA respectively.

同样刺激DPN对RVLM神经元的作用也随强度的改变而改变，在7只神经元也分别用100、200、300、400和500μA的电流刺激DPN，观察对其影响；只有强度增至500μA时才使所有神经元兴奋，且引起兴奋的潜伏期差别很大；个别神经元诱发放电的潜伏期也随强度改变。

Based on the return map and the principle of closed vectors, a new method is proposed to extract unstable periodic orbits embedded in chaotic attractors. As examples, the UPOs embedded in chaotic attractors of Logistic, Hénon and Lorenz are extracted respectively by this method. And our results of Skewed Hénon map also be compared with Nusse's. These results suggest that this method is valid for unstable periodic orbits from period one to period infinite of arbitrary dimension chaotic system. The dynamic considerations of spiking and UPO coding for individual neuron and neural system under external periodic and chaotic exciting stimulus also be studied in this dissertation. A lot of spiking phenomena, such as synchronization, period, and chaos appear alternatively with the changing of the stimulus frequency. For the small stimulus frequency the neuron could completely convey the periodic signal in synchronous anti-phase into interspike intervals sequences. For the slow time–scale chaotic input, the output two ISI sequences are reciprocally related to input signals, and their oscillation wave shape in time course can be derived from that of the input signals variation, furthermore, the similar input sequence and order of UPOs, distribution of LES and value of KYD remain in attractors reconstructed from ISI sequences.

发现周期信号在单个神经元传递过程中，随着激励频率的改变，神经元输出的峰峰间期interspike interval时间序列呈现出周期、混沌和准周期的交错变化，特别当外加激励信号频率较低时，周期信号可以通过神经元ISI序列以反相同步的周期运动形式传递下去；同时无论是周期还是混沌激励信号，在神经系统中的传递均与其自身强度和神经元之间的耦合强度的大小密切相关；快变时间尺度的混沌激励信号在耦合的神经系统传递过程中，会造成大量基本信息的丢失；而慢变时间尺度的混沌激励信号在神经系统传递中，它的非线性特征信息，如混沌吸引子、不稳定周期轨道、Lyapunov指数谱和分形维数，会通过系统输出的ISI序列部分地重现出来，如与输入慢变时间尺度的混沌激励信号相比，神经系统输出的ISI序列具有：相似几何形状的混沌吸引子、相近的Lyapunov指数谱和分形维数、局部结构相同的不稳定周期轨道的排列方式。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了