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We find that there are two types of CRF immunoreactive neurons in the cerebral cortex. Type I cells were mainly distributed in layers II -III. Morphologically this type of cells is interneuron and is the true CRF neuron in general meaning.

我们的研究显示,皮层中有两种类型的CRF阳性神经元,其中CRF阳性Ⅰ型细胞主要分布在皮层的Ⅱ-Ⅲ层,形态学特征显示这些细胞为抑制性中间神经元,且为真正的CRF神经元

First, the SVZa NSCs continuously migrate from SVZa to olfactory bulb in mammal whole life, form the rostral migratory stream and become the resource of OB interneuron renewing.

与其他部位的神经干细胞相比,SVZa神经干细胞还具有以下特点:(1)自产生时起即具备发育为神经元的特征;(2)迁移过程中保持增殖状态和神经元特征而不进一步分化;(3)到达迁移终点后,才进一步分化为DA能和GABA能神经元

The neonatal Wistar rats, clean degree, were used. The hippocampal neuron cells out of 9 rats were cultured and divided into the control groups of before and after hypoxia-oxygenation,and xionggui diwan 1 × 10-6 mol/L and 10 × 10-6 mol/L groups. Xionggui diwan was provided by the 88 Hospital of Chinese PLA and its active ingredient was the volatile ingredient of chuanxiong and danggui.

选择新生清洁级Wistar大鼠1只,取其海马,将其分离培养成海马神经元细胞,然后选取9例海马神经元细胞,将其放入3个培养皿中培养,每个培养皿中3例神经元细胞,将其分为缺氧-复氧前后、芎归滴丸(由解放军第八十八医院制剂室提供,主要成分为川芎和当归的挥发油)1×10-6mol/L组和芎归滴丸10×10-6 mol/L组。

Results After spinal cord hemisected, the number of CN and RN neurons was decreased obviously, the area of CN was diminished, and the NOS positive neurons of CN were increased at lesioned side in the control group.

结果 脊髓半横断后,对照组损伤侧背核神经元和红核神经元数目明显降低,背核面积减少,背核NOS阳性神经元增多。

It is widely known that during the development of nervous system, the synapse between neurons and target tissues plays an important role in the survival and maturation of neurons because target tissues or target cells can produce and secrete many kinds of neurotrophic factors and neuroinductive factors which can promote the growth, differentiation and migration of innervating neurons.

在神经系统发育中,神经元与相应靶组织建立的突触联系,是神经元存活和分化的关键因素,因为靶组织或靶细胞可以合成、分泌多种神经营养因子和神经诱向因子,促进支配神经元的生长、分化和迁移。

Results 45-51% of the nerve fibers innervating the superior rectus muscle in rats of group Ⅰ and 81-87% in group Ⅱ came from the neuron in contralateral midbrain, and the distribution of the neurons in midbrain was closer to normal rats in group Ⅱ than group Ⅰ.

结果 实验Ⅰ组大鼠支配上直肌的神经纤维有45%~51%由对侧中脑运动神经元发出;实验Ⅱ组81%~87%由对侧中脑运动神经元发出,神经元在中脑的分布更接近正常大鼠。

Results In contrast with enzymatic dissociation, the method of mechanical dissociation, breaking up great neurospheres into small spheres with gradually reduced pipette, had advantages of raising cell viability and shortening cell doubling time (P.05). After NSCs being cultured long term in vitro, the number of neurons generated decreased with passaging, whereas the number of astrocytes increased.

体外长期培养条件下,随着培养时间的延长,NSCs分化为神经元的比例逐渐降低,星形胶质细胞的比例逐渐增高;在4~12代NSCs分化为神经元的能力表现出下降的趋势,但仍较为稳定;12代后,神经元的分化比例迅速下降,星形胶质细胞的比例迅速上升。

The number of spinal motoneurons continued to decline in the first 3 months postoperativdy and plateaued after three months.

脊髓前角运动神经元数目在3个月内持续减少,3个月后趋于稳定,6个月时脊髓前角大型运动细胞坏死比率在40%左右,残存的神经元多为受损的神经元,尼氏体减少或消失。

Results: 1 The analysis revealed that GAP-43 immunopositive neurons in rats were localized in all the layers of lateral geniculate nucleus and the layers II -VI of visual cortex area 17,and GAP-43 is stained by a ring and dot figure and brown-yellow colour in cytomembrane ; 2, In the critical period, gene expression concentration and number of GAP-43 immunopositive neurons, in both the lateral geniculate nucleus and the visual cortex area 17, received from the deprived eyes, are reduced.

结果:1、GAP-43在正常大鼠视觉系统表达主要见于外侧膝状体全层和视皮质17区Ⅱ—Ⅵ层神经元胞膜中,呈环状或点状棕黄色免疫阳性反应;2、在敏感期内剥夺眼对侧外侧膝状体和视皮质17区GAP-43表达的免疫阳性神经元染色变淡(P<0.05),且免疫阳性神经元数目减少(P<0.05)。

Methods: The cerebral cortical astrocytes and neurons of rats were cultured and the model of simulated cerebral ischemia and reperfusion was set up in vitro. The activity, survival rate, death rate of the neurons, leakage rate of lactate dehydrogenase and the activity of nitric oxide synthase of cerebral cortical neurons were measured after 18 boors of reperfusion.

分离纯化培养大鼠大脑皮质Ast和神经元,体外模拟脑缺血再灌注损伤模型,用ACM培养损伤后的神经元,测定其神经元的活性、存活率、死亡率、培养液乳酸脱氢酶的漏出率和NOS强阳性细胞的表达量。

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