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The PRL-like cells were also presented in the ventral ependyma of the lateral ventricle and the glial lamina in the basal surface of the brain. The processes of PRL-LIR cells in PPN, SCN, PVN and PAN mainly projected to the third ventricle, those in AN and ELV mainly projected to the lateral ventricle, and those in SON to the glial lamina in the basal surface of the brain. The results showed that PRL could be released into ventricularis system and participate in the regulation of the cerebrum-cerebrospinal fluid circuit.

视前室旁核、视交叉上核、下丘脑室旁核、弓状核等核团内的PRL阳性神经元有突起向第三脑室投射,伏隔核内及侧脑室室管膜上的PRL阳性神经元有突起伸至侧脑室,视上核的PRL阳性神经元也有突起投射至脑基底神经胶质板,表明鸡脑内的PRL可以释放入脑室系统,参与调节脑-脑脊液神经体液回路。

METHODS: Sixty healthy Sprague-Dawley rats were randomly divided into 3 groups: 6 rats in normal control group, 6 rats in EGb treating normal IOP group, and the other 48 rats were established chronic high IOP models of rats by cauterizing two episcleral veins. The n 30 rats satisfying experimental level were selected and randomly divided into five groups: 6 rats in physiological brine treating group, and the other 24 rats were divided into four experimental groups according to the dose of EGb: group A, EGb 50mg/; group B, EGb 100mg/; group C, EGb 150mg/; and group D EGb 200mg/. After the treating time of 1 month, the rats were sacrificed on schedule.

取健康SD大鼠60只,正常对照组和正常银杏叶治疗组各6只,其余48只采用烧烙法,烙闭大鼠左眼2条浅层巩膜静脉,制作大鼠持续性高眼压模型,从中选出眼压稳定在实验要求水平的大鼠30只,随机分为生理盐水组,治疗A组(每日EGb 50mg/kg)、治疗B 组(每日EGb 100mg/kg)、治疗C 组(每日EGb 150mg/kg)、治疗D 组(每日EGb 200mg/kg),治疗时间为1mo,处死大鼠后做视网膜全层铺片,对RGCL神经元做特异性染色后行神经元计数分析来评估神经元的情况。

METHODS: Sixty healthy Sprague-Dawley rats were randomly divided into 3 groups: 6 rats in normal control group, 6 rats in EGb treating normal IOP group, and the other 48 rats were established chronic high IOP models of rats by cauterizing two episcleral veins. Then 30 rats satisfying experimental level were selected and randomly divided into five groups: 6 rats in physiological brine treating group, and the other 24 rats were divided into four experimental groups according to the dose of EGb: group A, EGb 50mg/; group B, EGb 100mg/; group C, EGb 150mg/; and group D EGb 200mg/. After the treating time of 1 month, the rats were sacrificed on schedule. Flat preparation of whole retinaes was stained distinctively and neuron counting in retinal ganglion cell layer from both eyes of each rat were performed to evaluate neuron situation.

取健康SD大鼠60只,正常对照组和正常银杏叶治疗组各6只,其余48只采用烧烙法,烙闭大鼠左眼2条浅层巩膜静脉,制作大鼠持续性高眼压模型,从中选出眼压稳定在实验要求水平的大鼠30只,随机分为生理盐水组,治疗A组(每日EGb 50mg/kg)、治疗B 组(每日EGb 100mg/kg)、治疗C 组(每日EGb 150mg/kg)、治疗D 组(每日EGb 200mg/kg),治疗时间为1mo,处死大鼠后做视网膜全层铺片,对RGCL神经元做特异性染色后行神经元计数分析来评估神经元的情况。

METHODS: Sixty healthy Sprague-Dawley rats were randomly divided into 3 groups: 6 rats in normal control group, 6 rats in EGb treating normal IOP group, and the other 48 rats were established chronic high IOP models of rats by cauterizing two episcleral veins. The n 30 rats satisfying experimental level were selected and randomly divided into five groups: 6 rats in physiological brine treating group, and the other 24 rats were divided into four experimental groups according to the dose of EGb: group A, EGb 50mg/; group B, EGb 100mg/; group C, EGb 150mg/; and group D EGb 200mg/. After the treating time of 1 month, the rats were sacrificed on schedule. Flat preparation of whole retinaes was stained distinctively and neuron counting in retinal ganglion cell layer from both eyes of each rat were performed to evaluate neuron situation.

取健康SD大鼠60只,正常对照组和正常银杏叶治疗组各6只,其余48只采用烧烙法,烙闭大鼠左眼2条浅层巩膜静脉,制作大鼠持续性高眼压模型,从中选出眼压稳定在实验要求水平的大鼠30只,随机分为生理盐水组,治疗A组(每日EGb 50mg/kg)、治疗B 组(每日EGb 100mg/kg)、治疗C 组(每日EGb 150mg/kg)、治疗D 组(每日EGb 200mg/kg),治疗时间为1mo,处死大鼠后做视网膜全层铺片,对RGCL神经元做特异性染色后行神经元计数分析来评估神经元的情况。

METHODS: Sixty healthy Sprague-Dawley rats were randomly divided into 3 groups: 6 rats in normal control group, 6 rats in EGb treating normal IOP group, and the other 48 rats were established chronic high IOP models of rats by cauterizing two episcleral veins. n 30 rats satisfying experimental level were selected and randomly divided into five groups: 6 rats in physiological brine treating group, and the other 24 rats were divided into four experimental groups according to the dose of EGb: group A, EGb 50mg/; group B, EGb 100mg/; group C, EGb 150mg/; and group D EGb 200mg/. After the treating time of 1 month, the rats were sacrificed on schedule. Flat preparation of whole retinaes was stained distinctively and neuron counting in retinal ganglion cell layer from both eyes of each rat were performed to evaluate neuron situation.

取健康SD大鼠60只,正常对照组和正常银杏叶组各6只,其余48只采用烧烙法,烙闭大鼠左眼2条浅层巩膜静脉,制作大鼠持续性高眼压模型,从中选出眼压稳定在实验要求水平的大鼠30只,随机分为生理盐水组,治疗A组(每日EGb 50mg/kg)、治疗B 组(每日EGb 100mg/kg)、治疗C 组(每日EGb 150mg/kg)、治疗D 组(每日EGb 200mg/kg),治疗时间为1mo,处死大鼠后做视网膜全层铺片,对RGCL神经元做特异性染色后行神经元计数来评估神经元的情况。

Based on Rough Set Theory, a neuralnetwork model of enterprise tacit knowledge communication isestablished and studied. Followingly this part discusses thedecision-making mechanism of enterprise tacit knowledgecommunication. R analyzes the multiple attribute decision-makings inenterprise tacit knowledge learning and a multiple attributedecision-making model is provided.

运用粗糙集理论对条件属性进行相对约简,通过去掉冗余条件属性,得到决策表的最小条件属性集和核,以决策表的某个约简作为输入层神经元,根据经验公式确定隐含层神经元个数,并以核元素连接对应输出层神经元,通过去掉冗余训练样本,提高沟通的学习效率,构建了企业隐性知识沟通神经网络优化模型。

NAA is the most sensitive marker of early brain damage decreased NAA correlate with prolongation of seizure time and loss of neurons; the change of Lac reflected moderate to severe injury of neurons;Increase in Cho may represent gliosis;decreased GABA can be detected when neuronal damage becomes severe.

1H MRS检出的NAA是反映早期脑损害的最敏感指标,其含量因癫痫持续时间延长和神经元数量减少而降低;Lac可以反映中等程度以上的神经元损害; Cho值升高,可能反映胶质增生;GABA值降低在神经元损伤较严重时可检出。

Results Apoptotic cells were found among normal dentate hilus cells and CA1 cells. Chromatin condensation and clumping could be seen in the apoptotic nuclei. The shrinkage of the nucleus was shown by foldings and whorls of the nuclear membrane. At late stage of apoptosis, the nuclear membranes could be broken.

结果 电镜下,实验组大鼠海马齿状回门区和CA1区内可见散在的凋亡神经元,凋亡神经元主要表现为核周染色体的聚集和凝结成块,其核膜表现为皱缩和扭曲,在晚期凋亡的神经元有时可见核膜破裂。

The plasticity of NSCs was detected by the differentiating test. HESCs were induced into dopaminergic neurons by means of adding Sonic Hedgehog Peptide and fibroblast growth factor eight (FGF8) early under the condition of mocked microenvironment and different development stage of dopaminergic neurons in vivo ,meanwhile made comparison with traditional method . The marker of dopaminergic neurons was tested by immunocytochemistry and RT-PCR, the content of dopamine and its derivation homovanillic acid were measured by HPLC-ECD.

模拟体内多巴胺神经元分化发育的不同阶段及微环境,采用早期添加音猥因子及成纤维细胞生长因子-8(FGF8)的方法,诱导hESCs定向生成多巴胺能神经元,并与传统的诱导方法相比较,免疫荧光细胞化学和RT-PCR法检测多巴胺能神经元的标志,高效液相色谱法检测诱导后细胞及细胞培养上清液中多巴胺及其代谢产物高香草酸的含量。

GABA-ergic neuron may involve in the modulation of acetylcholine releasing in spinal cord and in the thermoregulation in preoptic area and anterior hypothalamus,which may lead to the TPM related hypohidrosis.

4GABA能神经元可能参与节前胆碱能神经元调节、GABA能神经元参与丘脑下部对高温的反应。这种参与活动可能与TPM泌汗障碍相关。

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