神经元

There are totally three kind of neurons in the optic lobes: amacrine neurons, columnar neurons and tangential neurons, part of which are GABA immunoreactive and only several tangential neurons are 5-HT immunoreactive.

整个视叶共包含三种类型的神经元：无长突神经元、柱状神经元和切向神经元，它们中均有部分被GABA标记，而只有少数的切向神经元被5-HT标记。

After that we give a presentation of ion lysenkoism which describe the potential change between the two sides of membrane and present the Hodgkin-Huxley model and it\'s equations set.We introduce the basic conception of stochastic resonance and coherence resonance subsequently.

然后，给出了神经元的基本知识，包括神经元的基本结构，神经元细胞膜离子浓度的分布，离子电位梯度和平衡电位，神经元的静息电位、动作电位，接着本文给出了描述神经元膜两边电压变化的离子学说及Hodgkin-Huxley模型和它对应的方程。

The results showed that:(1) The characteristic frequency of IC neurons of house mouse was increased with increasing recording depth, and the masker intensity used was positive relevant to the IC neurons\' minimum threshold. When the minimum threshold was high, the masker intensity needed was loud, vice versa.(2) According to the masking rate at MT+10dB of IC neurons to frequency modulation and tone burst, the IC neurons were classified as three types: type I neurons ( 72/113, 63.7%), in which the masking effect of TB was better than frequency modulation ;type II neurons (32/113, 28.3%), in which the masking effects of the two maskers were same; type III neurons( 9/113, 8.0%), in which the masking effect of FM was better than TB.(3) Type I neurons, the minimum threshold of which was low, but the Q_(10) and DR values of which were large; in type III neurons, the minimum threshold was high, the Q_(10) and DR values were narrow; in type III neurons, the MT Q_(10) and DR values were in between.(4) Both FM and TB could make the MT of type I neurons shift upward distinctively, but the effect of TB was more significant than FM.(5) The Q_(10) values of type I neurons became narrower under the influence of TB, were unaffected under the influence of FM.

结果显示：(1)小鼠下丘神经元的特征频率随着记录深度的增加而增高，掩蔽声强度与神经元的最小阈值呈正相关，即对高最小阈值的神经元进行掩蔽时需要的掩蔽声强度高，对低最小阈值神经元进行掩蔽需要的掩蔽声强度低：(2)根据调频声(frequency modulation， FM)和短纯音(tone burst， TB)对下丘神经元阈上10dB处反应的掩蔽，将小鼠IC神经元分为三类：Ⅰ类神经元(72/113，63.7％)，短纯音的掩蔽效果比调频声更好；Ⅱ类神经元(32/113，28.3％)，两者对神经元声反应的掩蔽率相同；Ⅲ类神经元(9/113，8.0％)，调频声的掩蔽效果更好；(3)Ⅰ类神经元的最小阈值低、Q_(10)值和动态范围大，Ⅲ类神经元最小阈值高、Q_(10)值和动态范围小，Ⅱ类神经元的最小阈值、Q_(10)值和动态范围介于Ⅰ和Ⅲ类神经元之间；(4)短纯音和调频声都可以使Ⅰ类神经元声反应的最小阈值明显上移，但短纯音使神经元最小阈值的上移更显著：(5)短纯音使Ⅰ类神经元的Q_(10)值变小，调频声使神经元的Q_(10)值增大不显著，短纯音与调频声对神经元Q_(10)的影响差别显著；(6)短纯音与调频声都使神经元的动态范围减小，并且作用显著，但短纯音的效果更显著：(7)短纯音与调频声都使神经元反应的潜伏期延长，效果显著，但TB的延长作用更为显著；(8)随着探测声强度的升高，短纯音和调频声对Ⅰ类神经元声反应的掩蔽率都降低，但在各个探测声强度短纯音的掩蔽率都比调频声高。

Results In the control group, 68.6% motoneuron death of spinal cord occurred by 3 weeks following surgery, and the size of surviving motoneurons was significantly atrophy and NOS positive neurons increased. In the experimental group, the mortality was decreased by 35% as compared with that in the control group.

结果 对照组68.6%的神经元死亡，存活神经元胞体明显萎缩，同时表达NOS神经元增多；实验组的死亡率较对照组降低35%，存活神经元胞体无萎缩，表达NOS神经元未见增多。

Based on extended Linsker's network developed by Wimbauer et al.,we build a model for the receptive field of toad's R3 ganglion cell and a model for the receptive field of the nLM neuron of pigeon. Computer simulations on the 'head/tail preference' property,the configurational selectivity of the R3 neuron,and the selectivity for contrast,direction,orientation and the length of the moving edges of the nLM neuron,have been made. The simulation results are consistent with the physiological experiments qualitatively.

为揭示这类神经元的信息加工原理，以Wimbauer等人提出的时延Linsker网络为基础，通过将不同的时空动态感受野线性组合，建立了蟾蜍视神经节R3类神经元和家鸽扁豆核神经元的感受野模型，并模拟了R3神经元对蠕虫样刺激物的"头/尾偏爱"特征，细胞对刺激物的构型选择性以及nLM神经元对运动边缘的反差、方向、边缘朝向和长度的选择性。

The effective stimulating sites were indentified by defensive cardiovascular response and other components of defence reaction evoked by 3 s trains of rectangular cathodal pulses (100Hz, 0.5ms pulse duration, 70 -150μA intensity for dPAG and 150-250μA for PF) delivered from a programmed multichannel constant current stimulator. The responses included an increase in blood pressure and heart rate, pupillodilation and an increase in the rate and depth of respiration. Currents of 1Hz, intensity of 100- 150μA for dPAG and 200-250μA for PF were used to observe the effect of the stimulation of them on RVLM neurones. The deep peroneal nerve and superfacial deep peroneal nerve were carefully separated, and put on a pair of stimulating electrode respectively, Both nerves were stimulated with constant rectangular pulses of 1Hz, 0.5ms duration and 300μA current intensity to see their effects on RVLM neurones. The baro-and chemo-receptors were activated by the intravenous injection of phenylephrine or 0.208 M NaH2PO4. The degree of convergent inputs to individual RVLM neurone was studied by the stimulation of the sites mentioned above. It was found that neurones about 61.4%(27/44) and 35.3%(6/17) received convergent inputs from three and four places respectively.

过去的研究主要集中于整体水平，利用电刺激或化学刺激RVLM神经元，观察对血压、心率及交感活动的影响，并对影响心血管交感活动的神经递质进行了广泛探讨，积累了大量资料；在神经元水平，主要研究刺激脑内核团、外周神经、化学感受器及压力感受器等对RVLM神经元放电的影响和某些神经递质影响神经元活动的机制、然而，对RVLM神经元如何整合不同信息、其可能突触机制等研究得较少，本互作拟采用在体细胞内、细胞外记录电活动与微电泳，结合中枢核团及外周神经电刺激等方法，对RVLM神经元在不同状态下的整合作用规律与可能机制进行初步探讨，为进一步了解这一复杂的整合过程提供新的理论依据。

Third, it has a discussion on the development of modern and traditional artificial neural network technology and their application in image processing. It summaries that the model of the Pulse-coupled neural network based on Elkhorn's model of the cat visual cortex was constructed according to the mammalian vision systems. The nonlinear modulation property, which is a generic and pervasive biological pulse-coupling mechanism, is used in the construction of PCNN. So, PCNN finds many applications in image processing, including segmentation, edge extraction et al. As all known, the performance of the image segmentation depends not only directly on the adjustment of PCNN parameters and the statistical properties of image but also on the cyclic iteration times N of PCNN. If the parameters have been properly set, it turns out to be essential to select a suitable criterion to determine N.

回顾了神经网络技术的现状，指出传统人工神经网络只利用了生物神经元有限的属性进行模型构建，而90年代产生的脉冲耦合神经网络充分利用了哺乳动物视神经网络特有属性，其神经元模型除了考虑生物神经元特有的线性相加、兴奋与抑制属性外，还考虑到生物神经元特有的非线性相乘调制耦合特性；考虑了哺乳动物视神经系统视野受到适当刺激时，相邻连接神经元同步激发产生35Hz～70Hz振荡脉冲串特性等，所以PCNN网络模型更靠近真实生物视觉特性，特别是哺乳动物视神经网络，特别适合计算机图像处理应用，尤其在生物细胞图像分割、图像平滑、降噪及图像中目标的分类、计数等方面的研究。

Get the neonate Sprague-Dawley rats medulla spinalis, through the conventional primary cell culture procedure, we use the method of difference adherence time and get the motor neurons of anterior spinal cord. When the neuron is in maturity, we make the mechanical injury model in vitro. All the models were divided into four groups: group A is control group; group B is 100μM ATP group; group C is 100μM ATP+20μg/ml suramin group and group D is 100μM ATP+10μM ouabain+10μg/ml Thapsigargin group. Culture the four groups neurons, after one day, we count the motor neuron, observe the survival and activity of neurons through MTT shade selection experiment, use flow cytometry to analyze the percentage of apoptosis of motor neurons of anterior spinal cord and detect the expression of protein p-GSK-3β(ser9) through Western-Blot technology.

方法取新生大鼠脊髓，通过常规的原代细胞培养程序，采用差速贴壁法分离出大鼠脊髓前角运动神经元，培养成熟后制作神经元机械损伤体外模型，分为A组：对照组、B组：100μMATP组、C组：100gMATP+20μg/ml Suramin组和D组：100μM ATP+IOμM Ouabain+10μg/ml Thapsigargin组，对各组机械损伤的运动神经元进行培养，1天后分别进行运动神经元计数、MTT比色实验观察运动神经元的存活及活性、流式细胞仪分析机械损伤的脊髓前角运动神经元凋亡百分率和Werstern Blot技术检测p-GSK-3β(ser9)的表达。

Kangdai Ⅰ has protective function to the damaged neurons and astrocytes: Main results:(1) It has direct protective function to the damaged neurons. It can increase the activity and survival rate, decrease the mortality and the transudation rate of LDH in cultured medium and the strong positive cell count of NOS expression of injured neurons.(2) It has also directly protective function to the damaged astrocytes. It can increase the activity and survival rate and protein content in conditioned medium.(3) It can strengthen the ability of BDNF, GDNF, bFGF, HSP and IL-6expression in damaged astrocytes.(4) It can also strengthen obviously the expressions of NSE, bFGF-receptor and bc1-2, lower the expression of bax and caspase-3.(5) It can indirectly protect and restore the damaged neurons by astrocytes. Because the effect of ACMK (ACM interfered by Kangdai Ⅰ) is stronger than ACM+K (ACM associated with Kangdai Ⅰ).

抗呆Ⅰ号对受损的神经元和星形胶质细胞均具有保护作用：主要表现为：(1)对受损神经元具有直接的保护作用，可提高受损神经元的活性和存活率，降低细胞培养液LDH的漏出率、细胞死亡率和NOS染色强阳性细胞的表达量；(2)对受损的星形胶质细胞也有直接的保护作用，可提高其活性、存活率以及培养液蛋白质的含量；(3)能增强受损星形胶质细胞分泌BDNF、GDNF、bFGF、HSP和IL-6的能力；(4)可明显增强受损神经元对NSE、bFGF的受体和bc1-2的表达，降低受损神经元对bax和caspase-3的表达；(5)抗呆Ⅰ号可通过星形胶质细胞间接地保护和修复受损的神经元，因为在多数实验组中经抗呆Ⅰ号作用的ACM的作用远大于ACM与抗呆Ⅰ号联合应用的作用，统计学上具有显著性差异。

Type II cells were mainly distributed in layer V of cortex. It appeared that these cells receive the CRF immunostaining fibers terminals afferent from other cells rather than synthesize CRF by themselves. It is probable that type II cells receive afferent fibers from both extracortex and type I cells within layers II -III cortex. Considered that Type I cells morphologically are inhibitory interneuron, we presumed that type I in layer II -III could inhibit activity of type II cells in layer V.

CRF阳性Ⅱ型细胞主要分布在皮层第Ⅴ层，形态学特征显示这类细胞更像是接收来自其它神经元的CRF纤维投射而非自身分泌CRF，证据显示Ⅱ-Ⅲ层CRF神经元纤维可进入第Ⅴ层，考虑到皮层的CRF神经元形态上为抑制性中间神经元，这些结果提示Ⅱ-Ⅲ层CRF神经元能够抑制第Ⅴ层神经元的活动。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了