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AbstractWe present the early diagnosis method of agerelated macular degeneration , and characterize various pathological changes arising in the development of AMD, such as drusen, chorioretinal mapshrinking, retinal pigment epithelial atrophy, choroidal neovascularization, retinal pigment epithelium and neural epithelial detachment, and so on. We analyze its images by using optical coherence tomography.

本文给出了一种老年性黄斑变性(agerelated macular degeneration, ARMD )的早期诊断方法,在 ARMD 的发展过程中出现的各种病理改变如玻璃膜疣、脉络膜视网膜地图状萎缩、视网膜色素上皮萎缩、脉络膜新生血管、视网膜色素上皮/神经上皮脱离等,用光学相干断层成像技术(optical coherence tomography,OCT)对其进行图像分析。

AbstractWe present the early diagnosis method of agerelated macular degeneration , and characterize various pathological changes arising in the development of AMD, such as drusen, chorioretinal mapshrinking, retinal pigment epithelial atrophy, choroidal neovascularization, retinal pigment epithelium and neural epithelial detachment, and so on.

本文给出了一种老年性黄斑变性(agerelated macular degeneration,ARMD)的早期诊断方法,在ARMD 的发展过程中出现的各种病理改变如玻璃膜疣、脉络膜视网膜地图状萎缩、视网膜色素上皮萎缩、脉络膜新生血管、视网膜色素上皮/神经上皮脱离等,用光学相干断层成像技术(optical coherence tomography,OCT)对其进行图像分析。

The expression of nestin antigen, NSE and GFAP were identified by immunohistochemistry, and the number of NSE-positive cells and GFAP-positive cells were dividedly counted.

结果大鼠胚胎神经管神经上皮干细胞在无血清培养基中可形成大量呈nestin抗原阳性细胞构成的神经球,经传代有血清培养后分化为NSE阳性和GFAP阳性细胞,其中NSE阳性细胞占细胞总数的47.7%,GFAP阳性细胞占细胞总数的39.8%。

At histopathologic analysis, neuronal tumors are usually classified as pure neuronal cell tumors (gangliocytoma, Lhermitte-Duclos disease , central neurocytoma) and mixed neuronal-glial tumors (ganglioglioma, desmoplastic infantile ganglioglioma, dysembryoplastic neuroepithelial tumor, ganglioneuroma).

根据组织病理学的分析,神经元肿瘤通常被分为:单纯的神经元细胞肿瘤(神经节细胞瘤,Lhermitte-Duclos 病〔发育不良性小脑神经节细胞〕瘤),中枢神经细胞瘤)和混合性的神经元-胶质肿瘤(神经节神经胶质瘤,成结缔组织性婴儿节细胞胶质瘤,胚胎发育不良性神经上皮瘤,神经节瘤)。

Objective: To explore the influence of serum of different concentrations towards co-cultured neuroepithelial stem cells and Schwann cells, and further to seek the factors inducing the orientation differentiation of neural stem cells.

目的:探讨血清对联合培养神经上皮干细胞与Schwann细胞的影响,进一步寻求诱导神经上皮干细胞定向分化的影响因素。

Methods The neuroepithelial stem cells were isolated and cultured by microdissection, mechanical blowing and serum-free suspension culture.

方法采用显微解剖、机械吹打、无血清悬浮培养方法分离培养神经上皮干细胞,采用巢蛋白免疫细胞化学染色技术检测神经上皮干细胞,用NSE和GFAP免疫组化染色检测并计数神经细胞和神经胶质细胞。

Results: The Schwann cells promoted the survival and differentiation of neuroepithelial stem cells, and the number of the neurons decreased with an increase of serum concentration. Both the neuroepithelial stem cells and serum promoted the survival and the proliferation of the Schwann cells.

结果:Schwann细胞促进神经上皮干细胞存活和分化,随着血清浓度增加,神经上皮干细胞分化形成的神经元比例减少;神经上皮干细胞及血清均促进Schwann细胞存活和增殖,随血清浓度增加,存活及增殖率增加。

Results: Neuroepithelial stem cells derived from the embryonic neural tube could expand into neurospheres in the free serum medium and express nestin.

结果: GFP转基因鼠神经管来源的神经上皮干细胞在无血清培养基内能增殖形成神经球,呈巢蛋白阳性。

KCTD10 (potassium channel tetramerisation domain-containing 10); antibody cros s-reactivity ; immunohis-tochemistry ; neuroepithelium of neural tube ; dorsal root ganglion

KCTD10;抗体交叉反应;免疫组化;神经管神经上皮;背根神经节

Results: the findings on ct and mri were as follows: 57 cases of oligodendroglioma, 15 cases of ganglioglioma , 5 cases of dysembryoplastic neuroepithelial tumor , 3 cases of pleomorphic xanthoastrocytoma .conclusion:the images on ct and mri of cerebra peripheral tumor occur some characters , so ct and mri were certain worth on the diagnose and differentiate diagnose of cerebra peripheral tumor.

结果:少枝胶质瘤47例,间变性少枝胶质瘤10例,节细胞胶质瘤15例,胚胎发育不良性神经上皮瘤5例,多形黄色细胞瘤3例。结论:脑浅表肿瘤存在影像特征,ct及mri对其诊断有一定的价值。少枝胶质细胞瘤;节细胞肿瘤;胚胎发育不良性神经上皮瘤;多形性黄色星形细胞瘤;ct;mri

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