磺胺基的

The domestic demand of acetic acid up to 1,440,000-1,620,000 tons can be anticipated in 2010, if 60% is produced by methanol, 520,000 tons of methanol will be consumed;③Methanol can be used as feedstock to directly produce various pesticides ,such as fenitrothion, omethoate, dipterex etc., we anticipate that approximately 340,000 tons of methanol will be used to produce pesticides in 2010.④ Various medicines use methanol as feedstock, such as sulfanilamide, vitamin B6 etc., we anticipate that approximately 180,000 tons of methanol will be used to produce medicines in 2010.⑤ In the duration of dye production, methanol can be used as feedstock or solvent, such as s carlet base RC, disperse red GLZ, phthalocyanin ⑥ Synthetic resin also need methanol as feedstock such as organic glass, we anticipate that about 70,000 tons of methanol will be used to produce synthetic resin and plastics in 2010;⑦Synthetic fibers and synthetic rubber industry, will use methanol about 800,000 tons in 2010;⑧ At present, the production of MTBE is increase rapidly, there are 27 set of plants, total production capacity is 620,000 tons every year, and the demand is about 800,000 tons, there is gap between demand and supply.

预计2010年我国醋酸的需求量将达144～162万吨，按甲醇羰基法占60%计，将需求甲醇达52万吨；③多种农药的生产直接以甲醇为原料，如杀螟松、乐果、敌百虫等，预计到2010年甲醇用于生产农药约为34万吨；④多种医药药品以甲醇为原料，如长效磺胺、维生素B6等，预计到2010年甲醇用于医药约为18万吨；⑤染料生产过程中用甲醇作原料或溶剂，如红色基RC、分散红GLZ、阴率子GRL、酞菁素等等，预计2010年甲醇用量约为10万吨；⑥有机玻璃等合成树脂也需用甲醇为原料，预计到2010年甲醇用于生产合成树脂与塑料用量约为7万吨；⑦合成化纤与合成橡胶工业，2010年甲醇用量约为13万吨；⑧我国MTBE生产正处于快速增长期，现有MTBE装置27套，总生产能力62万吨/年，而年需求量约80万吨，有一定缺口。

P28 紫色 Formation of a peptide bond 肽键形成–4侧链具有的反应–形成二硫键-其它反应 Tyr,His,Arg Review Isoelectric Point Titration滴定 of an amino acid 滴定 Gly幻灯片 18 His Lys Glu Reaction of amino acids Section 4 Protein isolation and purification 蛋白质分离及提纯 Working With Proteins Experimental techniques for protein analysis and characterization Purification steps A cell contains many types of proteins In the lab we want to isolate a single protein for experiments Purification steps We first grow cells or isolate tissues that contain the protein of interest We break open the cells to produce a crude extract Use centrifugation离心 to separate soluble from insoluble material We fractionate 分离 the protein mixture based on properties of such as size, charge affinity or solubility.

丹磺酰氯与氨基酸反应生成荧光性质强和稳定的磺胺衍生物，用于多肽链NH 用于多肽链 3末端氨基酸的标记烃基化反应(1) 2，4-二硝基氟苯2，4-dinitrofluorobenzene，二硝基氟苯(二硝基氟苯 DNFB也叫做试剂。DNFB在弱碱性溶液也叫做Sanger试剂试剂中与氨基酸发生取代反应，生成黄色化合物二硝基二硝基苯基氨基酸(dinitro phenyl amino acid， DNP氨基酸氨基酸)苯基氨基酸氨基酸(2)苯异硫氰酸酯(phenylisothiocyanate， PITC)在弱碱性条件下，与氨基酸反应在弱碱性条件下，生成苯乙内酰硫脲 PTH衍生物，(phenylthiohydantoin， PTH)衍生物，即PTH-氨基酸，此反应又称之Edman反应，该反应是蛋白质或多肽氨基酸序列测定常用的反应。

The calibration curve of the SM2-Kit with standard SM2 inhibitor was typical sigmoid curve fitted to the four parameter logistic equation with the linear detection of 0.625 μg/L to 80.0 μg/L (R2=0.9911), the sensitivity of 0.9 μg/L, the IC50 of 5.82 μg/L and the detection limit of 1 μg/L. The recoveries of SM2 spiked in pig feed were 83.4 %, in pig urine were 89.5 %. The precision and accuracy of the assay as determined by inter-assay and intra-assay coefficient variation was both below 15 %. The SM2-Kit generally had 2.08 % cross-reactivity towards sulfamerazine and little or no cross-reactivity towards other sulfonamides. The dilution solution of SM2 had no effect on results of SM2-Kit. The validity of SM2-Kit in 4 ℃ was above six months.

研究结果表明，SM2-Kit的标准曲线呈典型的S型，相关系数R2=0.9911，符合4参数logit曲线拟合，线性检测范围为0.625 μg/L～80 μg/L，灵敏度为0.9 μg/L，半数抑制浓度(IC50)为5.82 μg/L，检测限为1 μg/L；饲料样、猪尿样的平均添加回收率分别为83.4 %、89.5 %，平均批内和批间变异系数均低于15 %；SM2-Kit与磺胺甲基嘧啶的交叉反应率为2.08 %，与其它磺胺类药几乎没有反应性；基质对SM2-Kit的检测结果影响不大；试剂盒在4 ℃可保存6个月。

Molecularly imprinted polymers of sulfamethazine(SM 2 ) were prepared with silica gel as a sacrificial material, methacrylic acid and 4 vinylpyridine(4 Vp) as functional monomers, glycol dimethacrylate as crosslinker. The polymer properties were investigated by static adsorption experiments. Scatchard analysis shows that the adsorption ability of MAA MIP is superior to that of 4 Vp MIP. The dissociation constant of MAA MIP is 9 59×10 -4 mol/L. The value of apparent binding a...

以磺胺二甲嘧啶为模板分子，分别用甲基丙烯酸和4乙烯基吡啶为功能单体，二甲基丙烯酸乙二醇酯为交联剂，采用牺牲硅胶法合成了磺胺分子印迹聚合物，通过静态吸附实验，系统研究了不同单体对聚合物识别性能的影响；Scatchard分析表明，以甲基丙烯酸为单体的聚合物具有较高的吸附能力，其离解常数Kd为959×10-4mol/L，最大表观结合位点数Qmax为3070μmol/g。

Three methods are used to prepare molecular imprinted polymers,which include conventional bulk technique or polymerization with ultraviolet initiated in low temperature and polymerization with silica-gel as sacrificial materials. In the polymerization procedure , Sulfamethazine(SM2)and Sulfisoxazole were used as target template molecules, methacrylic acid and 4-vinylpyridine(4-VP) and acrylamide were selected as functional monomers, ethylene glycol dimethacrylate as crosslinker, azobisisobutyronitrile was used as initator.

主要工作如下：首先，以磺胺异噁唑和磺胺二甲嘧啶为模板，分别以4-乙烯基吡啶，甲基丙烯酸，丙烯酰胺为功能单体，以二甲基丙烯酸乙二醇酯为交联剂，偶氮二异丁腈为引发剂，采用了传统方法(60℃恒温水浴)、牺牲硅胶法及低温紫外光聚合法等三种方法合成了一系列的磺胺类药物印迹聚合物。

Drugs (methimazole, methylthiouracil, propylthiouracil and thiouracil); some sedatives (doxylamine succinate and phenobarbital); and some other drugs including the systemic antifungal antibiotic griseofulvin, the diuretic spironolactone, and the antibacterial sulfa drugs sulfamethazine and sulfamethoxazole.

抗甲状腺？药物（他巴唑，甲基硫氧嘧啶，丙基硫氧和硫氧嘧啶）；一些镇静剂（doxylamine琥珀和苯巴比妥），以及包括系统性真菌抗生素灰黄霉素，安体舒通的利尿一些其他药物，以及抗菌磺胺类药物和磺胺二甲嘧啶。

A series of sulfanilamide group substituted derivatives were optimized on the basis of the semi-empirical quantum chemistry PM3 method.

以量子化学半经验PM3法对含磺胺基的一类有机化合物分子进行了几何构型优化，计算了分子的二阶非线性光学系数和电子转移，讨论了磺胺基在非线性光学材料设计中的特点和作用以及不同的取代基及取代位置对分子二阶非线性光学系数的影响

The 7-bromo group was replaced with various aniline, and then treated with 4-methoxybenzenesulfonyl chloride to afford the 6-azaindole-1-sulfonamides.The synthesis of 7-aryl-6- azaindole-1- sulfonamide derivatives were prepared by a Suzuki reaction at 7-position, utilizing the 7-bromo-6-azaindole was treated with a variety of phenylboric acid to give the designed 7-aryl substituted 6-azaindoles.

将七号位碳上的溴基取代成各种苯胺类衍生物，再与4-甲氧基-磺基氯苯反应得到6-氮基吲哚-1-磺胺。7-苯基-6-氮基吲哚-1-磺胺类衍生物的合成是以7-溴基-6氮基吲哚-1-磺胺为起始物和各种苯硼酸经由铃木偶合反应在第七号位碳上，得到在第七号位碳上有苯基取代的6-氮基吲哚-1-磺胺。

It was found that photochemical deposition of Hg occurred at the layer of TiO2 covering the surface of titanium electrode under UV irradiation, the adsorption of sulfadiazine on the photochemically deposited Hg was stronger than that on the electrochemically deposited Hg, and a method for the determination of sulfadiazine was proposed by the adsorptive stripping voltammetry based on photo-chemical deposition of Hg on Ti electrode.

该研究发现采用紫外光照射钛电极表面氧化钛层能够光催化还原Hg(上标致2+)生成Hg微粒，在Ti基电极上光化学沉积Hg比电化学沉积Hg对磺胺嘧啶具有更强的吸附作用，基于光化学沉积Hg/Ti电极建立了一种吸附溶出伏安法测定磺胺嘧啶的方法。

Secondly,UV spectrophotometry was adopted to research the combination action on SM_2 and SIZ with MAA and 4-Vp.The combination action of functional monomer with template molecules were produced in the results. Then,the prediction imprinting principle of SM_2 and SIZ were given. The diagrams of infrared spectrum were analysed in MAA and 4-Vp and IMPs of SM_2. It was discoved that the absorbability peak of C=C function group in the IMPs were turn to be very low ,but the function group of carboxylic acid and pyridyl in the IMPs were no evidence change, and the condition was created in the molecular recognition of MIPs. Thermal Analysis was adopted to research IMPs ,in which ,a better thermal stability and its decompose temperature were showed in the results.

其次，本文利用紫外分光光度法研究磺胺二甲嘧啶和磺胺异噁唑与甲基丙烯酸或4-乙烯基吡啶的结合作用，结果表明，溶液中的功能单体与模板分子之间产生了结合作用，并预测磺胺二甲嘧啶和磺胺异噁唑印迹机理；分别分析了功能单体甲基丙烯酸、4-乙烯基吡啶、磺胺二甲嘧啶和磺胺异噁唑分子印迹聚合物的红外谱图，经过对比发现制得的印迹聚合物中C=C双键峰很小，并且功能键羧酸键和吡啶氮没有明显变化，这为聚合物特异识别特性创造了条件；利用综合热分析仪，对分子印迹聚合物进行综合热分析，结果表明分子印迹聚合物具有较好的热稳定性，分解温度也较高。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。