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To detect the marks reflecting the liver functions as follows:①The marks to reflect the capability to synthesize proteins, TPROT, ALB, GLB;②The marks to reflect the injury of the liver cells: AST, ALT, CHE;③The marks to reflect the capability to egest the bile, excrect, and detoxicate: TBILI, DBILI, IBILI, TBA;④The enzymes to reflect the cholestasis: GGT, ALP. Results The results of both the morphological and the pathological studies showed that the degree of the liver cirrhosis was of the terminal stage.

肝功能指标:①反映肝细胞蛋白合成代谢功能的指标:总蛋白TPROT,白蛋白ALB,球蛋白GLB;②肝细胞受损的指标;谷草转氨酶AST,谷丙转氨酶ALT,胆碱脂酶CHE;③反映肝脏胆排泄、分泌及解毒功能的指标:总胆红素TBILI,直接胆红素DBILI,间接胆红素IBILI,总胆汁酸TBA;④胆汁淤积指示酶:谷氨酰转肽酶GGT,碱性磷酸酶ALP。

PAGE was used to analyze and compare the variation of composition and content of four isoenzymes , esterase, acid phosphatase, malate dehydrogenase and lactate dehydrogenase, of Paramecium bursaria which cultured in light and dark .

作者应用聚丙烯酰胺凝胶电泳技术分析比较了光培养绿草履虫和暗培养绿草履虫中酯酶、酸性磷酸酶、苹果酸脱氢酶和乳酸脱氢酶四种同工酶的组分及含量的变化。

For nearly thirty years the reactions of protein phosphorylation-dephosphorylation involving protein kinases and phosphoprotein phosphatases have been recognized as means of reversibly modulating the function of proteins.

数十年的研究表明,由蛋白激酶和蛋白磷酸酶催化的蛋白质磷酸化-去磷酸化反应是可逆地调节蛋白质功能的重要方式。

Potassium, natrium determines it is good that use ion chooses electrode indirect method it is better also with indirect method to determine at calcium of direct way;, it is next occasionally phosphor of; of law of azotic arsenic Ⅲ determines use phosphor molybdic acid is ultraviolet law outcome is better it is better with result of law of personal candy kinase that; blood sugar determines; urea determines use Niao enzymatic ultraviolet anhydride of better; flesh uses rate law result enzymatic law outcome is better; albumin determines, it is vanadium acid next alkalescent phosphoric acid of oxidation law; is enzymatic determine outcome of fluid of use AMP amortize is better; amylase determines law of thing of enzymatic standard background and iodic colorimetric law coefficient of variation all bigger, former a bit small, latter of percent of pass is tallish, each lab can try to choose according to his condition.

结论钾、钠测定使用离子选择电极间接法好于直接法;钙测定用间接法也较好,其次是偶氮砷Ⅲ法;磷测定使用磷钼酸紫外法结果较好;血糖测定用己糖激酶法结果较好;尿素测定用脲酶紫外速率法结果较好;肌酐用酶法结果较好;白蛋白测定用溴甲酚绿法较好;胆红素测定用酶法结果好,其次是钒酸氧化法;碱性磷酸酶测定使用AMP缓冲液结果较好;淀粉酶测定酶法底物法和碘比色法变异系数均较大,前者稍小,合格率后者稍高,各实验室可根据自己的条件加以选用。

The mount of germ and fungus quantity in soil are remarkably increased for the continuous cropping about 5 years, puts the trichobacteria quantity always change tendency to reduce;The edaphon total quantity, the bacterium quantity change reduces generally above the continuous cropping 5 years and below 5 years (including 5 years) the change are not obvious;In the nitrogen physiology group by ammoniates the bacterium primarily and the quantity changes are not very big;The nitrifying bacteria quantity is lower than the comparison (non- continuous cropping), the quantity of denitrifying bacteria is opposite;The alkalinity phosphatase, the urease, invertin activeness have a closed relations with the continuous cropping when it will increase less than 5 years or decrease in more than 5 years. The relation between catalase activeness and the continuous cropping is not very big. The reduction of the invertin activeness, the alkalinity phosphatase, urease activeness have slightly elevates, catalase activeness have no the regular changes.

表现为连作条件下土壤真菌数量显著增大,放线菌数量总的变化趋势降低;土壤微生物总量、细菌数量变化在连作5年以上普遍降低,5年以下(包括5年)则变化差异不明显;氮素生理群中以氨化细菌为主,但数量变化不大;硝化细菌数量低于对照组,反硝化细菌数量则相反:多酚氧化酶、转化酶活性在5年之前逐渐上升,5年之后逐渐下降,,脲酶活性随着连作年限增加持续上升,过氧化氢酶活性、碱性磷酸酶活性变化无规律。

U 2+, Co 2+ and Mn 2+ whose concentrations were 40, 40, 20μg·L -1 respectively could increase the AKP activity significantly in mysis, but higher con...

o2 +在 2 0μg·L-1时明显提高两种酶的活性。而Ni2 +只使两种酶活性降低。鉴于碱性磷酸酶和ATPase与生长发育直接相关,金属离子对对虾幼体生长的影响与对两种酶活性的影响基本一致,因此体内这两种酶活性的高低可作为判别环境因素是否适合对虾幼体生存的指标。

Correlation coefficients between ammonifying bacteria and urease, polyphenol oxidase, bacteria and phosphatase, total microbe quantity were significant at 0.05 levels, and correlation coefficients between aromatic decomposing bacteria and urease, catalase, phosphatase and total microbe quantity were significant at 0.01 levels.

相关分析表明,氨化细菌与脉酶、多酚氧化酶均呈显著正相关;芳香族分解菌与脉酶、过氧化氢酶呈极显著正相关;细菌、微生物总量分别与磷酸酶呈显著、极显著正相关。

Bt toxin from Bt crops is introduced into soil primarily in root exudates and by incorporation of crop residues after harvest of the crop, with probably some input from pollen during tasseling, which probably have an effect on soil specific organisms, biodiversity and the functions of soil ecosystem.

而加有转Bt基因玉米农大61秸秆的土壤和加有常规玉米农大3138秸秆的土壤相比,土壤脱氢酶活性和土壤呼吸作用强度在秸秆分解期间都没有显著差异;秸秆分解了30d、45d、60d 时,显著提高了土壤蔗糖酶活性;分解了45d和90d时,显著提高了土壤酸性磷酸酶活性;30d时显著增加了土壤脲酶活性,而在45d时则显著降低了土壤脲酶活性;分解45d、60d、90d时显著降低了土壤蛋白酶活性。

The soil microorganisms quantities, microbial diversity index, microbial biomass carbon and the cellulose decomposition strength significantly reduced with increasing of heavy metal concentration, but the soil basal respiration rate were positively enhanced. The soil urease, alkaline phosphatase and polyphenol oxidase in the lead zinc mining area were sensitive. The invertase and catalase were not obvious inhibited to the heavy metals.

该矿区土壤微生物区系组成和微生物活性显著不同于临近非矿区土壤,随着重金属含量的增加,土壤微生物数量、微生物多样性指数、微生物生物量碳以及纤维素分解强度均显著降低,但土壤基础呼吸却明显升高;土壤脲酶、碱性磷酸酶、多酚氧化酶对铅锌矿较为敏感,而蔗糖酶和过氧化氢酶受到的抑制作用不明显。

In order to investigate effects of bound residues of metsulfuron-methyl on the activities of soil enzymes, the activities of urease, acid phosphatase, catalase, and sucrase were determined in Huangfentuspiked with bound residues of metsulfuron-methyl for 98 days.

为了研究甲磺隆结合残留对土壤酶活性的影响,将黄粉土经甲磺隆结合残留处理98天,检测土壤脲酶、酸性磷酸酶、过氧化氢酶和蔗糖酶活性。

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