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Sodium malate and 1-Naphthyl phosphoric acid sodium were used as substrates. NBT and fast blue RR salt were used to stain MDH and ALP respectively.

鄄萘磷酸钠盐为特异性底物,氯化硝基四氮唑蓝和固蓝RR盐为染色剂,分别对MDH和ALP染色,以相对迁移率标记酶带。

METHODS: The subcutaneous adipose tissue was obtained from adult New Zealand rabbits under aseptic condition, and cultured in vitro with collagenase digestion. All cells were divided into 3 groups: in the BMP-2 group, cells were cultured with medium containing 0.1 g/L vitamin C, 10 mmol/Lβ-sodium glycerophosphate and 10μg/L BMP-2 for 10 minutes, followed by 4-14 days inoculation with density of 18×104 cells per pore. In the BMP-7 group, cells were cultured with BMP-7 with the same methods as BMP-2 group. The cells were cultured with simple culture medium in the control group.

无菌切取成年新西兰大白兔皮下脂肪,胶原酶消化法体外分离培养脂肪干细胞,分为3组:骨形成蛋白2组加入含0.1 g/L维生素C、10 mmol/L β-甘油磷酸钠、10 μg/L骨形成蛋白2的诱导培养基培育15 min,然后按18×104个细胞/孔接种,再培育4~14 d;骨形成蛋白7组培养方法基本相同,仅将骨形成蛋白2更换为骨形成蛋白7;对照组同法加入单纯培养基进行培育。

Omeprazole is a specific inhibitor of hydrogenpotassium ATP ase of the gastric parietal cell.

Omeprazole是对胃壁细胞之氢钾离子三磷酸腺核酶抑制剂。

It has very high heat stability, and may be used for preparing 1-phosphoglucose and oligosaccharide in different fields.

该酶具有极高的热稳定性,可用于医药、食品、生物工程领域1-磷酸葡萄糖及寡糖的制备。

Methods Platelet aggregations induced by ADP, AA and PAF, respectively, were measured by turbidimetric method after platelet incubated with 5'-nucleotidase.

采用比浊法测定白唇竹叶青蛇毒5'-核苷酸酶对二磷酸腺苷、花生四烯酸、血小板活化因子诱导血小板聚集的影响。

The addition of SHMP before spray-drying procedure could inhibit the activity of the protease and ensure the quality of the MTG preparation.

在喷雾干燥前添加六偏磷酸钠可以有效地抑制蛋白酶的活力,可以达到酶制剂的应用要求。

Diagnostic value of glucose-6-phosphate isomerase in patients with rheumatoid arthritis GONG Yi , ZHU Hong, Yu-rong Zhang The Affiliated Hosp.

导读:目的探讨血清中葡萄糖-6-磷酸异构酶(G6PI)抗原检测在类风湿关节炎患者中的诊断意义。

Effects of free fatty acid elevation on post absorptive endogenous glucose production and gluconeogenesis in humans.

提示它可以通过激活糖原磷酸化酶活力来加强小鼠肝细胞的糖原分解过程,促进肝细胞对葡萄糖的释放,从而使血糖升高。

PKA activators such as cAMP, 8 - Br -cAMP or phosphodiesterase inhibitor isomethyl butyl xanthine or purified PKA catalytic subunit all can inhibit germinal vesicle breakdown and meiotic maturation in mouse oocytes, also in Xenopus oocytes.

以小鼠卵母细胞为实验对象,给予PKA激动剂cAMP、8-Br-cAMP或磷酸二酯酶抑制剂IMBX均可抑制小鼠卵母细胞的胚泡破裂及减数分裂成熟。

Abstract—A yeast plasmid was constructed to contain a hybrid GAL–CYC promoter, the NPTII neomycin phosphotransferase gene, and the FRT sequence between them.

摘要,酵母质粒构建了含有混合放射公益少年团启动,新霉素的NPTII 磷酸转移酶基因,并首次登记税它们之间的序列。

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