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Other polysaccharides are formed from other sugar, which rose by enzymic transformations of phosphorylated hexoes and sugar nucleotides.

其它多聚糖则是由磷酸化己糖和核苷酸糖通过酶作用生成的其它糖聚合形成的。

An expression vector was constructed with a safe marker gene, 6-phosphomannose isomerase, and an insect-resistant gene, Galanthus nivalis agglutinin, was used in rice genetic transformation in the present study.

采用农杆菌介导法将来源于大肠杆菌的6-磷酸甘露糖异构酶安全选择标记基因和雪花莲凝集素抗虫基因构建于同一表达载体上进行遗传转化,获得带有安全标记基因的抗褐飞虱转基因植株。

Objective To study G6PD gene mutation diversity in Yao and Han people in Guangxi and compare the genotypic frequencies.

目的 研究广西瑶族葡萄糖-6-磷酸脱氢酶(G6PD)基因突变特点,并与汉族作比较。

The invention clones geranyl pyrophosphate synthase GGPPS gene from the ginkgo to construct plant expression vectors which contain ggpps gene, the ggpps gene is inducted into immature embryos of the ginkgo to induct out the callus tissue by the mediating of agrobacterium tumefaciens, PCR and semiquantitative RT-PCR detect the conformation and expression status of exogenous target gene ggpps, high-performance liquid chromatography and an evaporative light-scattering detector are employed to determine the terpene lactones content inside the callus tissue of the ginkgo, and the obtained callus tissue of the ginkgo of which the terpene lactones content is increased is screened.

本发明从银杏中克隆香叶基香叶基焦磷酸合成酶GGPPS基因,构建含ggpps基因的植物表达载体,用根癌农杆菌介导,将ggpps基因导入银杏幼胚并诱导出愈伤组织,PCR和半定量RT-PCR检测外源目的基因ggpps的整合和表达情况,高效液相色谱法及蒸发光散射检测器测定银杏愈伤组织中萜内酯含量,筛选获得银杏萜内酯含量提高的转基因银杏愈伤组织。

Their dynamic morphological changes,growth curve,glycerophosphate dehydrogenase pattern,oil red O staining,and reaction to insulin and dexamethasone all verified their preadipocyte identity.

经形态学动态变化、生长曲线、甘油磷酸脱氢酶活性变化、油红O脂肪染色及对胰岛素和地塞米松反应的测定,证明是功能活跃的前脂肪细胞,并在体外重现了其增殖、肥大的全过程。

Degradation test in vitro was carried out in phosphate buffer solution (0.1M, pH7. 4) at 37 ℃. The buffer solution was changed daily. Degradation test in vivo was implanted the sample to subdermal in adult ICH rat in the scapular area lateral to the dorsal midline. At suitable time the samples were recovered. Molecular weight changes in surface layer and bulk of polymer sample were measured by GPC and weight loss was determined gravimetrically. It was found that the degradation behavior can be regulated by changing the composition of copolymers. The critical compositions from surface to bulk degradation behavior for PGCA, PLCA, PLMCA, PLDCA systems were 15-20, 20-30, 30- 40, 40 of mol percent GA or LA unit in copolymers, respectively. The degradation behavior of PGCA, PLCA, PLMCA, PLDCA systems were compared and analysed. Some factors influencing the degradation character, such as copolymer composition, hydrophobicity, crystallinity and enzyme affect etc. played important role.

体外实验中材料降解环境为37℃,0.1M,pH7.4磷酸缓冲溶液,每天换液,定期取样;体内实验中将聚合物试样埋置于ICH小白鼠背部肩胛骨皮下部位,定期处死小鼠,取样,将体内体外样品进行重量损失及试样内外层分子量变化测定,分析各聚合物试样降解行为特性,实验结果证明,改变共聚物组成,可以调节各聚合体系降解行为特性,对PGCA,PLCA,PLMCA,PLDCA共聚体系,交酯摩尔百分含量15-20%,20-30%,30-40%,40%分别为各体系内降解行为特性由表面降解型向本体降解型过渡的临界转折点,交酯含量较低的聚合物不同程度地表现表面降解行为特性,论文对各共聚体系体内外降解行为作了分析对照,例如共聚物组成对材料降解速度与降解行为的影响;生物体内酶对降解行为的影响;材料亲疏水性,聚合物链段结晶行为及碳酸酯结构对材料降解行为的影响等,得出交酯/环碳酸酯共聚体系降解行为一些共性和规律。

These pathways include the production of advanced glycation end products, alterations in the sorbitol, hexosamine and protein kinase C pathways and activation of poly-ADP ribose polymerase.

代谢和血管通道的缺陷包括三个方面,一是糖基化终未产物形成,二是山梨醇、氨基己糖和蛋白激酶 C 改变,三是多聚二磷酸腺苷核糖多聚酶激活。

Thus, a vanadate inhibitable event , presumably a phosphotyrosine dephosphorylation event, was required for the initiation of differentiation process.

在用DEX,M1X和insulin诱导3T3-L1前脂肪细胞分化过程中,酪氨酸磷酸酯酶专一性抑制剂Vanadate则能有效阻断细胞分化的进程,但这种阻断是有时间限制的。

Oxidation system could play an important role in the blooming and aroma releasing of Jasminum sambac . External ethylene could not in2

明显的呼吸跃变现象,呼吸高峰出现在释香高峰前;氧化磷酸化解偶联剂和细胞色素氧化酶特异性抑制剂叠

Lamblia . Total genomic DNA was extracted and partial fragments of tim gene were amplified by PCR.

对不同来源虫株的磷酸丙糖异构酶基因进行 PCR扩增、序列测定后,用简约法和 NJ法构建分子系统树进行系统学分析。

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