磷酸酶

As a kind of potential drug for treating the Type II diabetes, the inhibitor of protein tyrosine phosphatase 1B (PTP1B) is an important research field, in which a kind of bidentate inhibitors is the research focus.

中国科学院研究生院，北京 100049摘要：作为二型糖尿病的潜在治疗药物，蛋白质酪氨酸磷酸酯酶1B（PTP1B）的抑制剂研究一直备受关注。

Butea Superba - High Flavonoids and Flavonoid glycosides with c-AMP Phosphodiesterase potent inhibitor Butea Superba is Twinning woody long life herbal plant exist only in Thailand from the central part, eastern part, western part till the northern part of Thailand.

化妆品木高黄酮和黄酮甙与c安培磷酸二酯酶抑制剂化妆品是木结木本长寿中草药植物只存在于泰国中部，东部，部分西方到泰国的北部地区。

In addition, we found that the cyclic nucleotide phosphodiesterase inhibitor IBMX greatly potentiates chronotropic, inotropic, and cAMP responses to CGRP in both left and right atria.

此外，我们发现磷酸二酯酶抑制剂IBMX大大增强了CGRP对左、右心房的变时、变力和cAMP效应。

Response surface analysis(central composite design of uniform precision) was applied to optimize the significant four factors[the ratio of bran and soybean meal,(NH4)2SO4, NaNO3 and KH2PO3] for culture medium of Aspergillus terreus by researching the contributiveness of factors and their interactions to the enzyme activity of acidic proteinase.

用响应面分析法（采用中心组合一致精度的设计）对影响土曲霉产酸性蛋白酶固态发酵培养基的4个主要影响因素（麸皮与豆粕比例、硫酸铵、硝酸钠、磷酸二氢钾）进行了优化，考察了各因素及其交互作用对酸性蛋白酶酶活的影响。

Dissolve the pancreatic digest of gelatin, the dibasic potassium phosphate, and the agar in the water, with warming, and allow to cool.

将白明胶胰酶消化物、磷酸氢二钾、琼脂溶解于水中，伴以加温，并静置放凉。

The results showed that glucose,ammonium sulphate,magnesium sulphate and dipotassium hydrogen phosphate were the main affecting factors.

部分因子实验设计结果表明葡萄糖、硫酸铵、硫酸镁及磷酸氢二钾为影响Saccharomyces cerevisiae B5产生高选择性羰基还原酶的四个显著性因素。

To investigate the expression, location and function, long distance PCR was used to expand the targets containing open reading frame. The expressive vectors of prokaryotic and eukaryotic cells were constructed after product purification and connecting with vectors. The prokayotic vectors were induced to express the protein by IPTG and the protein was seen by denaturalization PAG electrophoresis and dyeing. The eukayotic expressive vectors were transfected into culture cells and the protein was found in the nulceolus under the observation of the confocal microscope. The transfected cells were chosen by the geneticin (G418). The cell cyele was examined by flow cytometer and the cell numbers were reduced obviously in the stage of G〓-G〓 and G〓-M, but increased distinguishably in the S stage.

为研究磷酸化应激诱导蛋白的表达、定位和功能，采用了长距离聚合酶链反应扩增含有开放阅读框架的靶片段，经产物纯化、与载体连接，分别构建了STIP1基因的原核表达载体和真核表达载体；IPTG诱导原核表达载体，变性聚丙酰胺凝胶电泳与染色后，可见相应大小的蛋白质表达；STIP1基因的真核表达载体转染细胞系后，共聚焦显微镜下观察STIP1蛋白主要分布于细胞核内；用药物筛选STIP1基因转染的细胞后，流式细胞仪检测细胞周期，可见G〓-G〓期和G〓-M期的细胞明显降低，S期细胞明显增多。

Since gibberellin is one family of FPP derivatives in the diterpene pathway paralleled with sterol synthesis in the isoprenoid pathway.

对鲨烯合酶的下游产物之一胆固醇的检测显示，转基因烟草的胆固醇含量低于对照，同时以法呢基焦磷酸为前体的二萜支路产物之一的 GA3 含量得到提高。

METHODSequence analysis of Pyruvate orthophosphate dikinase from Echinochloa crusgalli var frumentaceaRoxb.

丙酮酸磷酸双激酶是一种在细菌、原生生物和高等植物中广泛存在的酶[1￣4]。

Understand and familiarize with clinical uses of β receptor blocker, emictory, PDE; phosphodiesterase suppressantin.

熟悉β受体阻断药，利尿药及磷酸二酯酶抑制药等在治疗充血性心力衰竭中的应用。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。