磷酸酶

A great quantity of phosphorylase mRNA wasdistributed in endosperm cells accumulating starch grains.

在积累淀粉的胚乳细胞中磷酸化酶mRNA大量分布。

Objective: To furthur study dual impact on thymidine phosphorylase mRNA expression and apoptosis in vitro in hepatocellular carcinoma cells by interferon-alpha.

目的 进一步研究干扰素-α对人肝细胞癌胸苷磷酸化酶表达水平及细胞凋亡的双重影响。

The activity of Ca2+-ATPase and phosphorylase A of liver in rats was determined to study the effect of acrylonitrile on calcium homeostasis and to clarify the toxicological mechanism of ACN.

本文通过丙烯腈对大鼠肝脏Ca2+-ATPase和磷酸化酶A活性影响的研究，探讨了丙烯腈对动物肝脏钙稳态的影响，进一步阐明丙烯腈的毒作用机制。

The function of hypothesis transport genes NMB0098 and NMB0097 were tried to study by way of gene knock out. The phototype of N. meningitidis strain BT878 was observed before and after gene NMB0098 was knocked out.

选用质粒pUC18为目的基因的载体，经过酶切、去磷酸化、连接、电穿孔法转导和抗生素筛选等步骤，分别将目的基因NMB0098和NMB0097与质粒相连，并在目的基因中插入标记基因—卡那霉素基因，构建盒式插入的重组质粒pNB0098-K和pNB0097-K。

Understand and familiarize with the classification of cholinergic agents, M effects, N effects, and also the pharmacological effects, clinical application and adverse reaction of physostigmine.

熟悉拟胆碱药的分类、M、N样作用、毒扁豆碱的作用、应用及主要不良反应；有机磷酸酯类中毒解救原则、胆碱酯酶复活药碘解磷定的作用机制和临床用途。

One such effect may be involved in the potentiation of the Iso relaxation of isolated guinea pig airways.

Nic和Theo一样，可能通过抑制磷酸二酯酶而增高Iso对气管平滑肌的舒张作用。

With a serial of dilutions, the standard curves of the threshold cycle relative to the log of each initial template copy of NAS1 and SPS gene were obtained, and the correlation coefficients were 0.99976 and 0.99571, respectively.

以蔗糖磷酸合成酶基因作为水稻的内源参照基因，通过梯度稀释法，分别获得了NAS1和SPS基因的Ct值与起始模板数的相关性标准曲线，相关系数分别为0.99976和0.99571，相关性高。

To produce the scientific evidence for developing and manufacturing new antitumor drugs.Methods: 1 The inhibitory effect on cell growth of Hela was measured by MTT assay in treated or untreated groups (3.125, 6.25, 12.5, 25, 50μg/ml TAM and control) for three different treatment times (24h, 48h and 72h).2 Apoptosis and cell cycle were measured by FCM in four experimental groups (0, 4, 16, 40μg/ml TAM) for 48h.3 Adopting Wright and Giemse's staining to observe the morphology of Hela cells which treated with 40μg/ml TAM.4 Using invasion experiment to detect the Hela cells'invasive abilities which treated with 40μg/ml TAM.5 The protein expressional levels of P-ERK, ERK, C-myc and Cyclin D1 in Hela cells untreated or treated with 4, 16, 40μg/ml TAM for 24h were measured by Western blot.6 Expression of anti-apoptotic gene bcl-2, apoptotic gene bax and MMP-9 in Hela cells of four experimental groups (0, 4, 16, 40μg/ml TAM for 24h), were observed by revers transcription PCR.7 The protein expression of P-ERK, ERK, Bcl-2 and Bax in Hela cells treated with 40μg/ml TAM for 24h observed by laser scanning microscopes.

1采用四甲基偶氮唑蓝法检测不同浓度北豆根总碱(3.125、6.25、12.5、25、50μg/ml)处理不同时间(24、48和72小时)对Hela细胞增殖反应的抑制作用。2采用流式细胞技术(flow cytometry，FCM)检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用48小时，对Hela细胞凋亡及周期变化的影响。3瑞氏-姬姆萨染色后显微镜观察北豆根总碱(0、40μg/ml)作用24小时后Hela细胞形态学变化。4采用Transwell小室法检测北豆根总碱(0、40μg/ml)作用24小时后对Hela细胞侵袭性的影响。5采用免疫印迹方法检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用24小时后，Hela细胞中磷酸化ERK、ERK、C-myc、CyclinD1的表达变化。6采用逆转录-聚合酶链反应(revers transcription PCR，RT-PCR)半定量检测北豆根总碱(0、4、16、40μg/ml)作用24小时，Hela细胞抗凋亡基因bcl-2、促凋亡基因bax、基质金属蛋白酶-9(MMP-9)的表达变化。7应用激光共聚焦显微镜(laser scanning microscope，LSM)观察北豆根总碱(0、40μg/ml)作用24小时后，Hela细胞内P-ERK、ERK、Bcl-2、Bax蛋白的表达变化。

This gene contained 1 530 bp and encoded 510 amino acids, named IbMIPS-1. Sequence analysis indicated that IbMIPS-1 had high homology with Ricinus communis MIPS gene, Nicotiana tabacum MIPS gene, Sesamum indicum MIPS gene and Glycine max MIPS gene.

该基因由1530个碱基组成，编码510个氨基酸，与蓖麻、烟草、芝麻、大豆肌醇－1-磷酸合酶的氨基酸序列比对表明，其同源性很高。

Intestinal microsomes of sea bream fed different lipid sources (fish, soyabean and rapeseed oils) at three different inclusion levels were isolated and incubated with l-[14C]glycerol-3-phosphate and [1-14C]palmitoyl CoA.

海鱼的肠微粒体用不同来源的油脂食物营养、用不同的含量，同时单独而且用碳14标记的3-磷酸甘油和棕榈酰辅酶A。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。