磷酸酶

Blasting in the GenBank, 16 of them were found that they have no significant similarity with data in GenBank, which means they were new sequences, but one sample(020) of them has high homology with data of Glyceraldehyde 3-phosphate dehydrogenase genes of other species in GenBank.

在GenBank中做Blast检索后，发现其中16条与数据库中序列无明显同源性，为新序列；一条序列(编号020)与数据库中其他物种的3-磷酸甘油醛脱氢酶基因高度同源。

Predict the effect on the net reaction catalyzed by glyceraldehyde 3-phosphate dehydrogenase if phosphate were replaced by arsenate.

预言：如果磷酸盐被砷酸盐取代，会对3-磷酸甘油醛脱氢酶催化的总反应产生何种影响？

Methods The real-time fluorescence PCR method for the detection of TSLC1 gene expression was established with designed specific primers, TaqMan probe and glyceraldehyde 3-phosphate dehydrogenase gene as control, and verified by cell lines and cervical tissue samples.

设计特异性引物和TaqMan探针，以3-磷酸甘油醛脱氢酶基因作为对照，建立检测TSLC1基因表达的实时荧光PCR方法，并采用细胞株和宫颈组织样本进行验证。

Prediction for secondary structure and function suggested that the 23rd amino acid residue-cysteine and nearby sequence is in a consensus pattern of Glyceraldehyde 3-phosphate dehydrogenase active site, in which the Cys is the catalytic residue, and there are some potential sites which may have biological signification in E20-GPD.

二级结构和功能预测分析表明，E20-GPD第23位的半胱氨酸及其附近序列为3-磷酸甘油醛脱氢酶活性位点一致序列，C为活性位点，第50位的组氨酸也是十分保守的，且E20-GPD具有多个可能的有生物学意义的位点。

Among the genes were glyceraldehyde 3-phosphate dehydrogenase, serine protease inhibitors, mitochondrion coding region, part of myosin heavy chain gene and two new genes, respectively.

结果 筛选出 6种蛋白分子基因：3-磷酸甘油醛脱氢酶，丝氨酸蛋白酶抑制剂，线粒体编码蛋白，肌球蛋白部分重链基因以及两个未知新基因。

Methods The total RNA of leucocyte was extracted 43 allergic vasculitis patients and 24 normal subjects before and after treatment with Trizol reagent and the integrity and concentrations of HLA-DRw53 gene mRNA were determined by agarose gel electrophoresis and UV spectrophotometer after denatured with 1% formaldehyde, the product was transcribed into complementary retroviral synthetic deoxyribonucleic acid, and sequence-specific primers were designed based on the sequences of second HLA-DRw53 gene exon and housekeeping gene glyceraldehyde phosphate-dehydrogenase cDNA.

利用随机引物逆转录合成互补脱氧核糖核酸，根据HLA-DRw53基因第二外显子序列和管家基因人磷酸甘油醛脱氢酶的cDNA序列设计序列特异性引物。检测43例变应性血管炎患者治疗前后与24名正常人HLA-DRw53基因mRNA表达量的变化。

Glyceraldehyde-3-phosphate dehydrogenaseis known for its pivotal role in glycolysisasa classical glycolytic protein in cytosolic energy production.

3-磷酸甘油醛脱氢酶作为一种糖酵解蛋白在糖酵解的能量产生中发挥着重要作用。

Glycerol obtained from the breakdown of fat is metabolized by conversion to dihydroxyacetone phosphate, a glycolytic intermediate, in two enzyme-catalyzed reactions.

来源于脂肪的甘油经两步酶促反应代谢转化为糖酵解的中间产物磷酸二羟丙酮。

The apparent Km values with guaiacol, catechol and pyrogallol as substrates were 2.75, 12.4 and 12.8mmol/L at 25℃ and pH 7.0, respectively.

该酶在25℃和0.05mol/L磷酸缓衝液(pH7.0)条件下对愈创木酚、邻苯二酚和没食子酸的Km分别是2.75、12.4和12.8mmol/L。

Objective To explore the relationship between postmortem interval and degradation of Hypoxanthine guanine phosporibosyltransferase mRNA in the kidney of rats.

目的：探讨大鼠死后肾次黄嘌呤鸟嘌呤磷酸核糖转移酶mRNA降解情况与死亡时间的关系。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。