磷酸酶

Objective To investigate the relationship between the two common glucose-6-phosphate dehydrogenase (G6PD) gene mutations in Chinese and their clinical manifestations.

目的 探讨中国人中两种常见葡萄糖-6-磷酸脱氢酶(G6PD)基因突变与临床表现的关系。

In blood, luteotropin significantly increased at DCAB 400 fed sample, estradiol (E2) and urea decreased at DCAB 400 and 500 fed sample. However, Lactate dehydrogenate, creatine phosphokinase and total protein had a significant increase for DCAB 300 and 400 fed sample.

血液指标中，DCAB 400组提高了促乳素，DCAB 400和500组提高了雌二醇浓度和尿素；DCAB 300和400组乳酸脱氢酶、肌酸磷酸激酶和总蛋白显著提高；处理组钙含量显著高于对照组。

Objective To investigate the correlation between the serum level of glucose-6-phosphate isomerase and autoimmune disease, and to explore the diagnostic value of GPI for AID.

目的 探讨血清葡萄糖－6-磷酸异构酶水平与各种自身免疫性疾病相关性及其诊断价值。

Objective To observe the expression of GAP-43 and the changes of malelic dialdehyde,nitric oxide and adenosine triphosphatase in the retinal tissues after optic nerve injury and to explore their function.

目的 通过对视神经损伤后，视网膜中生长相关蛋白-43（GAP-43）的表达及丙二醛、一氧化氮、三磷酸腺苷酶含量变化的分析，探讨上述物质在视神经损伤中的变化及作用。

Vector pDLBRBNPT contained plant selective marker gene nptⅡ and vector pCDMAR-Hyg contained plant selective marker hygramycin phosphotransferase gene , they fitted to dicotyledon and monocotyledon transformation respectively.

载体pDLBRBNPT含有植物选择标记基因nptⅡ，适合双子叶植物转化；而载体pCDMAR-Hyg的植物选择标记潮霉素磷酸转移酶(Hygramycinphosphotransferase，Hyg)分离，主要用于单子叶植物转化。

The study of triazole derivatives targeted IGPD is mainly reviewed, the enzymology and catalytic mechanism are also described in this paper.

文中介绍了IGPD的酶学特征与催化机理，并着重概述了以IGPD为靶标的三唑磷酸酯类抑制剂的研究进展。

The types of histone methyltransferases, the relationship between methylation of Lysine 9 of H3 and the formation of heterochromatin, gene regulation in euchromatin, and that with DNA methylation, were mainly introduced.

主要阐述了组蛋白甲基转移酶的类型，组蛋白H3中第9位赖氨酸甲基化与异染色质的形成、常染色体中基因表达的调控，以及与DNA甲基化之间的关系，说明了组蛋白甲基化与组蛋白乙酰化、磷酸化的相互关系，指出组蛋白甲基化对维持细胞各种状态的平衡起到极其重要的作用。

With electromicroscopic-cytochemical assay, comparative observation on the ultracytochemical localization of ATPase in the mesophyll cells of Euonymus radicans was carried out when the seedlings were cold-acclimated or not and then they were treated at -5℃ for 1d.

采用磷酸铅沉淀的电镜细胞化学方法，对经冷驯化（4℃处理）及未经冷驯化（22℃处理）的金边卫矛以及两者在-5℃下处理一天后的叶肉细胞进行了ATP酶的超微细胞化学定位的比较观察。

Three RNAi target sites (named as Abil1, Abil2, Abil3) targeting the E3B1 (NCBI: NM-024397), an identified target sites and positive control GAPDH-A were selected. The pGenesil-1 eukaryotic expression vectors with the neoR mark and GFP green fluorescent mark were selected to construct E3B1 RNAi plasmid. The effect of RNAi targeting various sites on E3B1 genes expression was evaluated by testing the transfection efficiency with fluorescence microscope and western blot. The most effective siRNA in inhibiting E3B1 genes were screened. The most effective siRNA in inhibiting E3B1 genes screened and used to transfect the neuronal cells. After that, the inhibitor of axon growth was added and the axon growth was observed. Result: The siRNA expressing plasmids targeting the E3B1 were constructed successfully.

选择针对E3B1(NCBI： NM-024397)RNAi的3个靶位点Abil1、Abil2、Abil3和1个不针对任何mRNA的RNAi靶位点以及甘油醛－3-磷酸脱氢酶，用带有neoR选择标志和GFP绿色荧光标志的真核表达载体pGenesil-1构建E3B1 RNAi质粒，分别转染培养的神经元，在荧光显微镜下观测转染率，经G418筛选得到单一的转染细胞，并用Western blot法检测各转染组神经元E3B1蛋白的表达情况，选出具有最佳抑制效应的siRNA转染神经元，加入轴突生长抑制物，观测轴突生长情况。

Ribose 5-phosphate and its derivatives are components of important cellular molecules such as RNA, DNA, NAD+, flavine adenine dinucleotide, ATP and coenzyme A.

5-磷酸核糖和它的衍生物是细胞内分子如RNA、DNA、NAD+、黄素腺嘌呤二核苷酸、ATP和辅酶A的主分成分。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。