磷酸酶

Activities of chymase and ACE were tested by radioimmunological method. Expressions of phosphoric extracellular signal regulated kinase 1/2 (p-ERK1/2) in hearts were measured by western blot. Results: As compared to the control group levels of GSP, myocardial enzymes and myocardial Ang Ⅱ were much lower in those of APS group.

心肌组织的血管紧张素Ⅱ含量；RT-PCR法检测心肌糜酶和血管紧张素转换酶 mRNA表达；放免法检测chymase和ACE的活性；免疫蛋白印迹法检测心肌磷酸化的细胞外信号调节激酶1/2(p-ERK1/2)含量。

GSK-3βis now recognized as not only a key rate-limiting enzyme of glycogen metabolic process, which phosphorylats hepatic glycogen synthase and makes it devitalize by insuline regulation, but also plays an important role in at least three signal transductory systems, namely, the Wnt/wingless, PI3'-kinase and Hedgehog pathways,which influence cellular processes, such as protein synthesis, cell proliferation, cell differentiation, cell motility and apoptosis.

GSK-3β是糖原代谢过程中的重要限速酶，除了可以在胰岛素调控下磷酸化肝糖原合成酶并使之失活外，GSK-3β还在多个重要信号通路中扮演了关键角色，例如Wnt/β-catenin pathway，PI3K/AKT pathway，Hedgehog pathway，在蛋白合成、细胞增殖、细胞分化、细胞运动、细胞凋亡以及肿瘤发生等方面都扮演了重要角色。

The protein is then transported by specialized cesicles to a late endosome that later matures into a lysosome . The mannose 6-phosphate receptor recycles back to the Golgi for re-use .

溶酶体蛋白质即由特异的囊泡运输到后内吞体中，最终形成溶酶体。6-磷酸甘露糖受体回到高尔基体上重新使用。

Methods The concentrations of serum PCⅢand angiotensinⅡ were measured by radioimmunoassay in 43 patients with AMI, the serum concentrations of creatine kinase isoenzymes MB also were measured by enzym immounoassay at the same time.

采用放免法检测 4 3例AMI患者血清PCⅢ、血管紧张素Ⅱ(angiotensinⅡ，AngⅡ)水平，酶免疫测定法检测肌酸磷酸激酶同工酶MB水平。

Moreover, we evaluated the inhibitory series in enzymic kinetics, selectivity among other phosphatases and two related cellular models.

针对这样一系列化合物，我们还在酶动力学、与其他磷酸酯酶选择性和细胞水平上进行了全面的评价。

With the aid of PDQuest 2-DE software, 11 significant protein dots were found out and then 9 of them are pick out to go through peptide mass fingerprinting based on MALDI TOF MS and NCBI database searching . In all, 5 proteins were identified: Ribulose bisphosphate carboxylase , Nucleic acid-binding protein , Ribosomal protection tetracycline resistance protein , hypothetical protein FG04726.1 [Gibberella zeae PH-1], nitric oxide synthase .

应用PMF技术对这11个具有显著表达差异蛋白点中的9个进行鉴定，得到了5个质谱阳性鉴定结果，其中2个蛋白分别为玉米种属的1，5-二磷酸核酮糖羧化酶／加氧酶和叶绿体的核酸结合蛋白；其它的3个蛋白在玉米蛋白质数据库中未见报道，但与其它物种基因编码的蛋白质具有高度的序列同源性。

This product can suppress the activity of PDE III, reduce the infiltration of oil, and promote the synthesis of hormone-sensitive type esterase, thus has the effects of decomposing and releasing lipocyte lipid.

抑制磷酸二酯酶Ⅲ活性，减少油脂渗入，并促进激素敏感型酯酶的合成，从而具有分解和释放脂肪细胞内脂质的作用。

The oxidase system includes both membrane-bound and soluble cytosolic proteins. The primary feature of this enzyme is stimulus-dependent activation and which mechanism is the phosphorylation and translocation of a complex of cytosolic factors to the membrane, where they associate with a flavocytochrome enzyme.The fully assembled complex functions as an electron transport system,moving electrons from cytosolic NADPH to molecular oxygen to form superoxide anion（O2-）, which, along with subsequent reactive products called respiratory burst,exerts microbicidal and cytotoxic activities.

此氧化酶体系包括膜结合的和可溶的胞浆蛋白，其主要特征为依赖刺激而活化，机制为复合体的胞浆因子磷酸化并转位至膜，在这里与细胞色素酶相关联，组装完全的复合体在功能上作为一个电子转运体系，从胞浆NADPH转移电子给分子氧形成超氧阴离子（O2-），伴随着接下来的活性物质产生，也叫呼吸爆发，发挥抗菌和抗细胞毒素的活性。

Isolation and purification and molecular characteristic of a myrosinase ,which was isolated and purified from the extraction by ammonium sulfate,DEAE-52 and Sephadex G-100 chromatography were studied.

以茎用芥菜为原料，采用磷酸缓冲液浸提、(NH4)2SO4盐析、DEAE-52和Sephadex G-100色谱对其中的芥子苷酶予以分离纯化，并就该酶的分子特性进行了研究。

The branched nature of glycogen makes it more accessible to glycogen phosphorylase during degradation, since this enzyme degrades the molecule by sequential removal of glucose residues from the nonreducing ends.

糖原的支链性质使它在降解时更易接近到糖原磷酸化酶，因为这种酶降解分子是从非还原端依次去除葡萄糖残基。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。