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Tissue samples were examined on 0, 7, 14, 21, 28 and 35 d with micro section paraffin and histochemistry staining method.

分别于0,7,14,21,28和35d取材,利用显微石蜡切片、组织化学染色的方法检测其肝、消化道和肌肉细胞糖原、酸性磷酸酶和碱性磷酸酶细胞化学特征。

Tissue samples were examined on 0, 7, 14, 21, 28 and 35day with micro section paraffin and histochemistry staining method.

分别于0、7、14、21、28和35 d取材,利用显微石蜡切片、组织化学染色的方法检测其肝、肠和肌肉细胞糖原、酸性磷酸酶和碱性磷酸酶细胞化学特征。

The level of tumor necrosis factore alpha, interleukin-6 (IL-6) and interleukin-8 (IL-8) were evaluated using ELISA method. Western blotting was used to detect the protein expression of protein kinase B and lipid phosphatase PTEN. Immunoprecipitations and radioactivity of γ〓P incorporated into its specific substrate histon H〓B was utilized to determine the PKB activation. Phosphate concentration released from PTEN-specific substrat diC16PIP3 was assessed using green reagent method.

以细胞计数、噻唑蓝比色法测定细胞增殖能力,酶联免疫法检测培养上清液中细胞因子TNFα、IL-6、IL-8水平,Western免疫印记定量蛋白激酶B、蛋白磷酸酶PTEN表达水平,免疫沉淀、特异底物组蛋白H2Bγ〓P掺入量测定PKB活性,特异底物dic16PIP3脱磷酸绿色试剂法反应PTEN活性。

The results show that lipid in megagametophyte is preferentially used during germination than lipid from embryo or seedlings and starch accumulation in cotyledon and hypocotyl functioned as temperate storage material.

在子叶中细胞质和质体果糖1,6二磷酸酶的活性都明显高于下胚轴,根和未萌发胚中细胞质和质体果糖1,6二磷酸酶的活性极低,表明子叶和下胚轴进行活跃的蔗糖和淀粉合成活动。

The activities of catalase, polyhenol oxidase, urease, protease, saccharase of grafted eggplants were higher than those of the control, but the activities of peroxidase and phosphatase were obverse.

嫁接茄子的过氧化氢酶、多酚氧化酶、脉酶、蛋白酶、蔗糖酶的活性均高于自根茄子,但过氧化物酶和磷酸酶的活性自根茄子高于嫁接茄子。

Methods seventy-two 15-week-old sd rats were divided into six groups,included control group and five ovariectomized model groups.the model groups were fed with different-dose lycopene[10,15,20 mg/] for 12weeks.then related indexes including length bone mineral density,bone mineral,serum estradiol,sertum alp and uterus index were analyzed.results compared with model group,three different dose groups and nilestriol[1.05mg/] group showed increased bone mineral density,bone mineral,serum estradiol and uterus index,but decreased serum alp (p.05).conclusion as a female sex hormone-like component,lycopene may ameliorate the bone quality and inhibit osteoporosis in ovariectomized rat.

将72只15周龄sd大鼠随机分为6组,空白组做假手术,其余5组做卵巢切除术,术后用药12周后处死,测定右侧股骨骨密度、骨矿含量、血清雌二醇、碱性磷酸酶含量及子宫湿重。结果番茄红素高[20mg/]、中[15mg/]、低剂量组[10mg/]、尼尔雌醇组[1.05mg/kg·bw]能对抗骨密度、骨矿含量、血清雌二醇的下降、血清碱性磷酸酶的升高,与模型组比较,p.05;番茄红素各剂量组、尼尔雌醇组能对抗子宫指数的减轻,与模型组比较,p.05。结论番茄红素可以改善骨质量,抑制大鼠去卵巢骨质疏松的发生。具有类似雌激素的效应。

A complex sample containing BMP and sodium fluoride was implanted under the abdominal skin of young male Wistar rats.

在纯化的BMP中加入氟化物再植入大鼠皮下,通过比较植入物在不同植入时间的组织学变化,碱性磷酸酶、酸性磷酸酶活性及钙含量来反映BMP的生物活性。

Protein tyrosine phosphtases are a superfamily of structurally and functionally related enzymes for dephosphorylation of pr...

蛋白质酪氨酸磷酸酶是催化蛋白质去磷酸化的一类庞大的酶家族,在细胞信号传导及代谢的调节中起重要作用。

The EDTA inhibition dynamics of Ulva pertusa Kjellm alkaline phosphatase and calf intestine alkaline phosphatase were the complexing irreversible inhibition, but these two were different in the inhibition process.

EDTA对孔石莼碱性磷酸酶和小牛肠碱性磷酸酶的抑制动力学均为络合型不可逆抑制,但是二者在抑制过程中具体情况稍有不同。

Results The bone mineral density of Lumber vertebra and trachanter and BGP were increased and bone pain was alleviated and serum ALP,ACP were significantly decreased and the blood,urine routine and biochemical quota were no obvious change in Bugu capsula group.

结果 补骨胶囊组腰椎及转子骨密度均升高,骨痛明显改善,与此同时,骨钙素显著提高,血清碱性磷酸酶、酸性磷酸酶明显降低,治疗后血尿常规及生化指标无明显改变。

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