磷酸酶

Twenty-four hours after the last oral administration, the blood were obtained and used for testing adrenocorticotrophic hormone, cyclic adenosine monophosphate, cyclic guanosine monophosphate, glucose, cholesterol, alanine aminotransferase, urea nitrogen. Results Before taking medicine, model rats' cAMP/cGMP and ACTH were lower than normal rats', and they showed a trend of rise with adding dose. Before taking medicine, model rats' GLU was lower than normal rats'. With adding dose, model rats' GLU increased, however, normal rats' GLU reduced. When taken medium dose white prepared lateral root of aconite, model rats' CHO was higher than normal rats'. Taking medicine made both groups ALT rise, but normal rats' went up more quickly than model rats'. Model rats' BUN was higher than normal rats', and taking low dose or little dose medicine could lead to incline of reduction.

结果 给药前模型组环磷酸腺苷/环磷酸鸟苷和促肾上腺皮质激素低于正常组，随着给药剂量的增加，两者有升高的趋势；给药前模型组血糖低于正常组，随着给药剂量的增加，模型组GLU有升高的趋势，而正常组有下降的趋势；中剂量给药时模型组总胆固醇显著高于正常组；随着给药剂量的增加，2组丙氨酸氨基转移酶均有升高的趋势，且正常组ALT升高的幅度更大；给药前模型组尿素氮高于正常组，低、小剂量给药有降低模型组BUN的趋势。

An enzymatic method for the determination of fructose _ 1,6 diphosphate in plasma was established in order to study its pharmacokinetics.

建立了用酶法测定血浆中1，6_二磷酸果糖的方法，为含1，6_二磷酸果糖制剂的药代动力学研究提供基础。

This process was studied by following the substrate reaction of GPa in this paper.

本文第五章用底物反应动力学的方法研究了糖原磷酸化酶的磷酸化过程。

Effect of albendazole upon activity of phosphoenolpyruvate carboxykinase,pyruvate kinase,fumarate reductase,isocitric dehydrogenase, malate dehydrogenase of Cysticercus cellulosae in swine bodies had been determined in the experiment .

测定了在阿苯达唑作用下猪体内囊尾蚴磷酸烯醇式丙酮酸羧激酶、丙酮酸激酶、延胡索酸还原酶、异柠檬酸脱氢酶、苹果酸脱氢酶活性的变化。

SOD, ATPase, MDH and ME isoenzymes in gill, digestive gland, foot, adductor muscle and mantle of Mytilus edulis were tested and analyzed using polyacrylamide gel electrophoresis.

采用聚丙烯酰胺垂直电泳法对紫贻贝 Mytilus edulis 的鳃、消化腺、外套膜、足、闭壳肌等5种组织的超氧化物歧化酶、三磷酸腺苷酶、苹果酸脱氢酶、苹果酸酶进行了检测分析。

The Charon library DNA was then digested with restriction enzymes BamH Ⅰ and Hind Ⅲ, and DNA fragments of length of about 0.5-1. 5kb was subcloned into plasmid pUC 18 which was also digested by BamH Ⅰ and Hind Ⅲ and dephosphorylated by alkaline phosphatese before being ligated with Charon fragments.

抽提λCharon文库总DNA，用BamHⅠ和HindⅢ完全酶切电泳，用低熔点琼脂糖回收长约0.5-1.5kb的DNA片段；连接到pUC18上，pUC DNA先用BamHⅠ和HindⅢ酶切，后用碱性磷酸酯酶脱磷。

By now, there is seldom report on the Heilongjiangwood frog which hibernates at low temperature condition. Our study mainly analyze theadaptation changes of liver proteins, SOD, LDH, G6PDH and EST activities of Heilongjiangwood frog under low temperature.

本文旨在研究低温条件下黑龙江林蛙肝脏蛋白质、超氧化物歧化酶、乳酸脱氢酶、葡萄糖6-磷酸脱氢酶(G6PDH)及酯酶的适应性变化，以探讨黑龙江林蛙冬眠期低温耐受的机制，为两栖类低温生物学和比较生理学的深入研究提供理论参考和实验依据。

Metal complex and metallomicelle as model compounds for artificial enzyme have been widely and extensively studied. In this dissertation, a series of homo- and hetero-dinuclear oxamido-bridged complexes have been synthesized, and the catalytic hydrolyses of carboxylic acid esters and phosphate by these complexes have been systematically investigated, and the catalytic mechanisms have also been dissected.

金属配合物及金属胶束作为人工酶的模型化合物受到广泛而深入的研究，本文通过合成一系列的草酰胺桥联同核和异核双核配合物，较为系统地研究了它们催化羧酸酯、磷酸酯水解反应的动力学，探讨了它们的作用机制；同时，本文还探索了将金属胶束这一较为成功的水解酶模拟模型应用于过氧化物酶模拟研究的可行性。

As an important enzyme in both glycolysis and glyconeogenesis, D-Glyceraldehyde-3-phosphate dehydrogenase has been shown to be an allosteric enzyme, The yeast enzyme binds 〓 with positive cooperativity, whereas the muscle enzyme binds 〓 with negative cooperativity.

D—甘油醛—3—磷酸脱氢酶是生物体内葡糖酵解与糖原异生过程中的关键酶，不同种属的酶在协同效应与半位反应方面表现出较大差异。

To determined the role of this gene in gluconeogenesis and in pathogenesis of Xcc, mutants of pgi gene were constructed by homology plasmid integration method.

六磷酸葡萄糖异构酶是糖异生途径中另一个关键酶，在野油菜黄单胞菌中编码该酶的基因是pgi基因(Xcc 8004基因组中基因编号为XC2379)。

By the time of its fall, most of the prisoners were writers who had written against the corruptions of the government.

到它被攻陷的时候，里面多数的犯人是写了反对政府贪污文章的作家。

The most obvious variation to ovum morphological character was that the color was changed from light green to sepiaceous in embryonic development, and all the ovums were almost hatched after 96h.

在胚胎发育过程中卵的形态特征最明显的变化是颜色从淡绿到深褐色，卵在发育96h后卵基本全部孵化。