磷酸酶

Our subordinate companies exclusively distribute chemical raw material awarded from province and ministry such as 10,000-30,000 unit amylase, protease, high grade different propyl alcohol, cymene naphthalene, glacial acetic acids, Adipic acid, emulsifier, Sodium hexametaphosphate, industry alcohol, etc.

下属分厂专业生产省优部优淀粉酶1-3万单位、蛋白酶、植酸酶、木聚糖酶、优质异丙醇、甲基萘、冰醋酸、α-乙酰乳酸脱羧酶、乳化剂、六偏磷酸钠、工业酒精等化工原料。

Sequencing revealed that the most common genes appearedin the library is photorespiratory-related genes, such as ribulose bisphosphate carboxylase, Rubisco activase, glyoxylate aminotransferase, glutamine synthetase,glyceraldehyde dehydrogenase, ferredoxin, transport protein, etc.

测序之后发现最为显著的是一些光呼吸相关的基因如核酮糖-1，5-二磷酸羧化／加氧酶、Rubisco激活酶、乙醛酸转氨酶、谷氨酸转氨酶、甘油醛脱氢酶、铁氧还原蛋白和转运蛋白等。

To explore the function of PDE in canine central nerve , gene expression of 18 PDE subtypes in different fractions of canine central nerve (cerebrum,cerebellum,myelencephalon and spinal cord) were detected by RT-PCR,and PDE activity was calculated by the content changes of cyclic nucleotides before and after the PDE reaction examined by high performance liquid chromatography.

为探明磷酸二酯酶(phosphodiesterase，PDE)在犬中枢神经活动中的作用，采用反转录聚合酶链反应检测18个PDE亚型在犬中枢神经不同部位中(大脑、小脑、延髓和脊髓各段)的表达分布，以高效液相色谱法检测环核苷酸在酶反应前后的含量变化，计算PDE活性。

The first step of the plant pterin branch is catalyzed by GTP cyclohydrolase Ⅰ and the first step in the conversion of chorismate to pABA is mediated in plants by aminodeoxychorismate synthase.

叶酸分子是由喋呤和对氨基苯甲酸从头合成的，二者合成的关键酶腺苷三磷酸环化水解酶和氨基脱氧分支酸合成酶已得到克隆鉴定。

The expression and activity of the thiamine-dependent enzyme, transketolase--the pacemaking enzyme of the reductive pentosephosphate pathway, is consequently decreased.

维生素B1依赖性酶（转酮醇酶——还原型戊糖磷酸循环的限速酶）的表达及活性下降。

Also, some enzymes are named according to the type of reaction they catalyze, such as phosphorylase or dehydrogenases.

此外，某些酶是根据它们所催化的反应类型来命名的，例如磷酸化酶或脱氢化酶。

Purified protein samples were separated by 2-DE with 17 cm IPG strips, and the protein spots were detected by fast silver staining. Six protein spots were randomly excised from the silver stained gel for primary analysis by MALDI-TOF MS. Four proteins were identified, and they were 1-aminocyclopropane-1-carboxylate synthase 2, ribulose-1, 5-bisphosphate carboxylase/oxygenase large subunit, F11O4.2 and Rubisco small subunit.

根据预实验结果，选用17 cm IPG预制干胶条对纯化的蛋白样品进行2-DE实验，采用快速银染法进行染色，随机挖取银染胶上6个蛋白点进行MALDI-TOF MS分析和数据库检索，鉴定了3个蛋白，它们是：1-氨基环丙烷-1羧酸合酶2，核酮糖-1，5-二磷酸羧化酶/加氧酶大亚基和F11O4.2。

However the addition order of tartrate and phosphate on activity of enzyme adsorbed by goethite produced the greatest impact.

胶体表面类型（硅氧烷表面或羟基化表面）及阴离子配体对胶体表面负电荷的贡献量在酶的吸附及活性保持中的重要性；B阴离子配体后加入时酶的吸附量一般比配体先加入时的高；C）配体加入顺序对二氧化锰的影响最显著，对砖红壤和高岭石的居中；对针铁矿的次之；对黄棕壤的最小。D）乙酸或草酸加入顺序对高岭石上固定化酶比活性的影响最大，而酒石酸或磷酸则对针铁矿影响最大。

After thecomplete genome extraction of the strain was performed, the genomic DNA was partiallydigested by restriction enzyme Sau3AⅠ, the DNA fragments from 1 to 5Kb was clonedinto prokaryote expression vector pET-28a-c, and transformed host bacteria. The resultsshowed that we succeeded in constructing the gene expression library of haemophilusparasuis serovar 5, which is fundamental for the study of advanced gene screening. Inaddition, primer design was performed based on haemophilus influenzae in this study. In addition, PCR was performed by using genomic DNA of haemophilus parasuisserovar 5 as the template. The results demonstrated that we obtained two neo-gene:23SrRNA gene(conserved gene belonging to the large-subunit of ribosome) and adenylatecyclase gene(encodes adenylate cyclase and participates in converting adenyl nucleosidetriphosphate to cyclic adenosine3",5"-monophosphate). Furthermore, the phylogeneticanalyses between the species was performed, and neighbor-joining tree was constructedbased on comparison of 23S rRNA gene sequences, so it was illuminated betweenHaemophilus parasuis and other species in molecular evolution relationship.

选择我国流行优势菌株副猪嗜血杆菌血清5型地方株为研究对象，提取细菌基因组DNA，用限制性内切酶Sau3AⅠ对基因组DNA进行部分酶切，回收大小为1～5Kb的DNA片段，将其连接入原核表达载体pET-28a-c，最后转化宿主菌，结果成功地构建了基因表达文库，为后续的基因筛选工作奠定基础；另外，本研究选择嗜血杆菌属的流感嗜血杆菌为参考对象进行引物的设计，以副猪嗜血杆菌血清5型地方菌株的基因组DNA为模板，进行PCR扩增反应，结果表明成功地获得两个新基因：23S rRNA基因(存在于核糖体大亚基中的保守性基因)和腺苷酸环化酶基因(负责将腺嘌呤核苷三磷酸转变为环腺苷酸)，并进一步做了不同物种之间的分子系统发育分析，构建了基于23S rRNA基因的邻接法系统发育树，阐明了副猪嗜血杆菌与其它菌种的分子进化关系。

The catalytic action of various kinds of functional coordination compound and metallomicelles which are made from the complex and micelle, as mimic hydrolase or peroxidase, have been studied respectively in catalyzing the carboxylic ester and phosphatic diester hydrolysis, and the phenolic derivant oxidation in this paper. Some novel results of academic significance have been obtained.

本文以不同类型的功能配合物及其与表面活性剂形成的金属胶束作为水解酶和过氧化物酶的模拟酶模型，分别研究它们对羧酸酯、磷酸二酯水解反应和酚类氧化反应的催化作用，取得了新的有学术意义的成果。

By the time of its fall, most of the prisoners were writers who had written against the corruptions of the government.

到它被攻陷的时候，里面多数的犯人是写了反对政府贪污文章的作家。

The most obvious variation to ovum morphological character was that the color was changed from light green to sepiaceous in embryonic development, and all the ovums were almost hatched after 96h.

在胚胎发育过程中卵的形态特征最明显的变化是颜色从淡绿到深褐色，卵在发育96h后卵基本全部孵化。