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The effects of pH value, RNase from malted barley roots and the added proteinase on the yeast autolysis wereresearched.

在酵母核酸酶的催化性质中,仅有pH稳定性和温度稳定性与麦芽根核酸酶(5'-磷酸二酯酶)的性质有较大差别。

Ribulose 1,5 bisphosphate carboxylase/oxygenase regulates photosynthesis and photorespiration. It is a key enzyme to control the net photosynthesis in plants.

1 ,5二磷酸核酮糖羧化酶/加氧酶是调节光合和光呼吸,决定净光合作用的一个关键酶;也是植物可溶性蛋白质中含量最高的蛋白质。

Objective To study the effects of different doses of manganese on activities of GS and PAG in the rat striatum and the activities of SDH and Na^+-K^+-ATPase in pallium in order to explore the cell molecular mechanism of manganese-induced exeitotoxicity.

目的研究不同剂量锰对大鼠纹状体谷氨酰胺合成酶、谷氨酰胺酶和脑皮质琥珀酸脱氢酶、钠钾三磷酸腺苷酶(Na^+-K^+-ATPase)活性的影响,以探讨锰的兴奋性毒性的细胞分子学机制。

The biosynthesis of the quinoid nucleus are the same in all coenzyme Qs. The biosynthesis of the isoprenoid side chain determine the kind of coenzyme Q.

芳香环的合成在所有的辅酶Q族物质中都是相同的,异戊烯基侧链的长度则决定了辅酶Q的种类,这里存在一个关键酶,即异戊烯焦磷酸合成酶。

The purpose of this study is to reveal the change characteristics of biological properties of sandy soil and its development trend under the establishment of C. microphylla plantations in flowing sand dune. With semi-moving sand dune and natural community as controls, the activities of phosphomonoesterase, saccharase, protease, urease, dehydrogenase and the Contents of microbial biomass C, N, P of the soil in an age sequence (5-, 10-, 22-year) of C.

为探讨采用小叶锦鸡儿固定流沙后,土壤生物活性的变化特点和发展趋势,选取5、10、22年生小叶锦鸡儿人工固沙群落为对象,以半流动沙丘和天然群落为对照,研究了人工固沙群落发育过程中土壤磷酸单酯酶、蔗糖酶、蛋白酶、脲酶、脱氢酶的活性和微生物生物量C、N、P质量分数的变化特征。

Enzymes of glycolysis (hexokinase,lactate dehydrogenase,aldolase) showed low activities during overwintering when more carbon flowing into treh alose synthesis.

越冬期间体内糖原磷酸化酶活性明显地增加,糖酵解有关的酶(己糖激酶、乳酸脱氢酶和醛缩酶)活性较低,以保证更多的碳源转化成海藻糖。

Methods Myocardial ischemia was induced by subcutaneous injection of ISO (30 mg/kg for two days, once a day). The experimental animals were randomly divided into the normal group, ISO group, positive control group and three crocetin groups (25, 50, 100 mg/kg). Cardiac indexes were examined. The level of LDH, CK, MDA in serum were measured. The content of MDA, GSH-Px and the ATPase activity heart and mitochondria were assayed by colorimetric analysis. The histopathological change of myocardia was investigated by HE staining.

采用大剂量ISOsc诱导产生大鼠急性心肌缺血模型(1SO30mg/kg,每日一次,连续2d),实验大鼠随机分为正常组、ISO模型组、阳性对照组和3个西红花酸给药组(25,50,100mg/kg),测定心脏系数和血清中乳酸脱氢酶、磷酸肌酸激酶和丙二醛含量,观察心肌组织谷肮甘肤过氧化物酶、Na^ ,K^ —ATP酶和Ca^2 ,Mg^2 —ATP酶活性及心脏病理改变,观察西红花酸的保护作用。

This method conformed the following conditions: spleen phosphodiesterase and micrococcal nulcease for digesting reagent and the concentration, temperature and digesting time of the two enzymes;the components of p32-post label alleviant and labeling time;the concentration of p1 nuclease; the concentration of T4 polynucleotide kinase for catalysing and labeling reagent;the components of bidirectional spreader and the spreading time ,etc.

确定了采用脾磷酸二酯酶和微球菌核酸酶消化底物以及两种酶的使用浓度、温度及消化时间;确定了P32标记缓冲液的主要成分及标记时间;确定了P1核酸酶的浓度;确定了T4多核苷酸激酶催化标记底物的浓度;确定了双向展开剂的主要成分及展开时间等。

Compared with the ck, the sugar accumulation increased extremely significant by grafting with the wild watermelon 2, but that of the other treatments all decreased and the sugar accumulation of loofah/Zaojia was the lowest. The sucrose invertase activity decreased; the activitives of sucrose phosphate synthase and net for sucrose-metabolism enzymes all increased during the development of own-rooted and grafted watermelons.

与对照相比,野生西瓜2号/早佳组合的果实糖积累极显著增高,而其他嫁接组合的糖积累均降低,丝瓜/早佳组合的果实糖含量最低;自根和嫁接西瓜果实发育过程中蔗糖转化酶活性降低,蔗糖磷酸合酶活性升高,蔗糖合酶在西瓜果实发育后期主要起合成作用,促进果实蔗糖积累,蔗糖代谢相关酶净活性值增大。

Feeding for 24h by third instar larvae of the maize stalk borer induced farnesyl pyrophosphate synthase gene expressing, but had no effect on expression of AOC, BX9, PAL and PR2a genes and DIMBOA content.

3玉米螟3龄幼虫处理24h能诱导玉米根中法呢烯基焦磷酸合成酶基因表达,但不能诱导丙二烯氧化物环化酶、β-1,3葡聚糖酶基因(PR2a)、糖基转移酶(BX9)、苯丙氨酸转氨酶基因表达,同时对第二叶和根中丁布含量无显著影响,使第二叶对羟基苯甲酸及第三叶丁布、对羟基苯甲酸、香草酸含量显著降低。

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