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磷酸酶

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Up-regulated proteins under salt stress include an ABC transporter permease, glycerol-3-phosphate permease, pyrimidine nucleotide transporter, formate dehydrogenase, and down-regulated proteins include succinate dehydrogenase iron-sulfur subunit, flavoprotein subunit, cytochrome b-556 subunit, and a hypothetical membrane protein similar to charperone DnaJ. Some of these changes were further verified by enzyme activity assay.

盐胁迫诱导上调表达的蛋白包括ABC型转运蛋白,3-磷酸甘油透性酶,嘧啶核苷转运蛋白和甲酸脱氢酶;下调表达的蛋白包括琥珀酸脱氢酶铁硫亚基、黄素蛋白亚基、细胞色素b556亚基,以及分子伴侣DnaJ的同源蛋白,酶活力测定结果表明胁迫条件下上述蛋白的活性变化与表达量变化相一致。

The oxidation of glyceraldehyde 3-phosphate to 1,3-bisphosphoglycerate, catalyzed by glyceraldehyde 3-phosphate dehydrogenase, proceeds with an unfavorable equilibrium constant, yet the flow through this point in the glycolytic pathway proceeds smoothly .

3-磷酸甘油醛氧化成1,3-二磷酸甘油酸,被3-磷酸甘油醛脱氢酶所催化,按照一个热力学不利的平衡常数进行,但是糖酵解途径中反应可以平稳地通过这一点。

The strawberry fruit were sampled from 1 to 8 weeks after full blossom. The contents of sucrose, glucose and fructose and the activities of sucrose phosphate synthase, sucrose synthase, acid invertase, neutral invertase, hexokinase and fructokinase were measured in developing strawberry fruits.

采集花后1~8周的果实,分别测定果实中葡萄糖、果糖、蔗糖的含量和蔗糖磷酸合酶、蔗糖合酶、可溶性酸性转化酶、中性转化酶、己糖激酶及果糖激酶等蔗糖和己糖代谢酶的活性。

The total number of colony forming units of culturable bacteria and fungi were increased significantly(p.01 in transgenic sugarcane rhizosphere soil, but The total number of CFUs of culturable was not effected. No significantly higher CFUs of bacteria, fungi and actinomycetes resistant to kanamycinwere obtained in transgenic sugarcane soil.

结果表明:转基因甘蔗显著(p.05)降低了土壤脱氢酶的活性,提高了土壤的纤维素酶、蛋白酶和脲酶活性(p.01),而对蔗糖酶和磷酸单脂酶没有影响;转基因甘蔗显著增加了根际土壤可培养的细菌和真菌数量,但对放线菌影响很小。

In these chloroplasts carbon dioxide combines with phosphoenolpyruvate to form oxaloacetic acid, which is transported to the bundle sheath cells, where the carbon dioxide is released, then fixed by the enzyme ribulose bisphosphate carboxylase to form glycerate 3-phosphate, the first step in the Calvin cycle.

在叶肉细胞的叶绿体中二氧化碳与磷酸烯醇丙酮酸结合形成草酰乙酸,后被运到邻近的维管束鞘细胞,在那里二氧化碳被释放,后被核酮糖二磷酸羧化酶固定形成3-磷酸甘油酸,这是卡尔文的循环第一步。

A diverse range of effects:on protein solubility and secretion was observed,and these effects were highly dependent on the particular chaperone.In the host strain over-expressing chaperone SecB harboroing pSecB,the amount of total periplasmic proteins was increased by about 71%and the activities of alkaline phosphatseand GL-7-ACA acylase(GL7A)were increased by about 54%and l.5-fold respectively.In the host strain over-expressing chaperone GroEL bearing pGroEL,the amount of total periplasmic proteins was increased by 52%,and the activity of penicillin G acylasewas in creased by about 76%;about 90%of Hexameric Calcitonin(Cal6)inclusion body and l5%of MS2interleukin3(MS2-HIL3)inclusion body became soluble respectively.All the data were compared with the parental strains un-bearing plasmdis pSecB or pGroEL.

在过量表达SecB的宿主菌中,周质空间分泌蛋白总量较对照组提高了约71%,GL-7-ACA酰化酶在周质空间酶的活力较对照组提高了约1.5倍,碱性磷酸酯酶在周质空间酶的活力较对照组提高了约54%;在过量表达GroEL的宿主菌中,周质分泌蛋白总量较对照组提高了约52%,青霉素G酰化在周质空间酶的活力较对照组提高了约76%,鲑鱼降钙素六聚体的可落性组分的比例由原来的45%增加到约90%,而MS2-人白介素-3融合蛋白的包涵体有约15%转变为可溶性组份。

Fruits of mango cultivar Yuexi No.1(Mangifera indica L.) at the mature stage were collected and detected in terms of starch, glucose, fructose and sucrose, as well as acid invertase, neutral invertase, sucrose synthase and sucrose phosphate synthase.

以粤西1号为试材研究了芒果果实成熟阶段淀粉、葡萄糖、果糖和蔗糖的含量以及蔗糖代谢相关酶-酸性转化酶、中性转化酶、蔗糖合成酶和蔗糖磷酸合成酶的活性变化。

Trehalase plays an important role in glycogen accumulation in the formarion of diapaused eggs. At the beginning of diapause, glycogen phosphorylase is the key enzyme to glycogen decomposition and the formation of sorbitol and glycerol, whereas at the stage of diapause termination, NAD-sorbitol dehydrogenase is the key enzyme to sorbitol decomposition and glycogen re-synthesis.

在滞育卵形成过程中,海藻糖酶对糖原的积累起着重要的作用;在滞育开始时,糖原的分解与山梨醇、甘油的形成,糖原磷酸化酶是关键酶;在滞育解除时,山梨醇的分解与糖原的再生成时山梨醇脱氢酶(NAD-sorbito1 dehydrogenase)是关键酶。

A series of pH- and temperature-sensitive hydrogels carrying pendant phosphategroups have been synthesized by copolymerizing 2-methacryloyloxyethyl dihydrogenphosphate with N-isopropylacrylamide. The swelling ratio, swelling and deswellingkinetics have been investigated. High loading percentage of lysozyme into hydrogelshas been achieved and the release profile has been studied.

通过N-异丙基丙烯酰胺和含磷酸基团的单体2-甲基丙烯酰乙氧基二氢磷酸酯的共聚合,合成了一类含磷酸基团的既对温度敏感又对pH敏感的水凝胶,研究了它们在不同pH及温度时的溶胀率及溶胀动力学,并初步研究了其对溶菌酶的释放行为。

Overview Glycogen degradation and glycogen synthesis are controlled both by allosteric regulation and by hormonal control.

变构控制和共价修饰磷酸化酶存在有磷酸化的活性 a 型河渠磷酸化的正常情况下无活性的 b 型。

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