磷酸酶

Two gene fragments of DEN-1(GenBank accession number DQ886390)and DEN-2(GenBank accession number DQ886391),differentially expressed between the ocular skin tissues of normal and albino turbot,were isolated by mRNA differential display and sequenced.It was confirmed by RT-PCR method that the expression levels of both DEN-1 and DEN-2 were down-regulated in the ocular skin tissue of albino turbot.The sequence homology search of DEN-1 revealed high sequence homologies with the UGGT genes of zebrafish and cattle,and the Ugcgl1 gene of Norway rat.High sequence homologies of DEN-2 were observed with the eya4 gene(the eye absent homolog 4)from red jungle fowl,zebrafish,human,Norway rat,mouse and dog.

应用mRNA差异显示技术，对比研究正常与白化大菱鲆有眼侧皮肤组织，克隆差异表达基因，经测序，RT-PCR 验证，差异表达基因片段DEN-1(GenBank登录号：DQ886390)与DEN-2(GenBank登录号：DQ886391)均在白化大菱鲆有眼侧皮肤组织中下调表达；通过BLAST检索发现，DEN-1与GenBank中的斑马鱼和牛的类似尿苷二磷酸-葡萄糖：糖蛋白葡糖基转移酶基因、与挪威鼠类似尿苷二磷酸-葡萄糖：神经酰氨葡糖基转移酶1(Ugcgl1)基因有较高同源性；DEN-2 与原鸡、斑马鱼、人、挪威鼠、家鼠和狗的眼缺乏同源框4(eya4)基因的同源性均较高。

The identified proteins were divided into two following categories: one is regulatory proteins, for example, caffeic acid 3-O-methyltransferas, HSP70, glyoxalase I , actin, and so on. They are involved in protein biosynthesis and processing, cellular structural organization and stress responses. The other is function proteins, for instance, ascorbate peroxidase, cytoplasmic malate dehydrogenase, cytosolic phosphoglycerate kinase, S-adenosyl methionine synthetase, ATP synthase, and so on. They are involved in photosynthesis, photorespiration, redox homeostasis, and metabolisms.

通过分析可以将这些差异蛋白分为两大类：第一类是调节性蛋白，如咖啡酸三氧转甲基酶，热激蛋白70，醛酮变位酶I，肌动蛋白等，它们主要参与蛋白质的合成和加工、胁迫响应、细胞骨架稳定等过程；第二类是功能性蛋白，如抗坏血酸过氧化物酶，细胞质苹果酸脱氢酶，细胞质磷酸甘油酸激酶，S-腺苷蛋氨酸合成酶，ATP合酶等，它们则参与氧化还原平衡、光合作用、光呼吸和各种代谢等过程。

Cancellous bone from the lumbar proteomic expression analysis show that actin, keratin, enolase, ATP synthase, the expression of myosin may be related to ovarian lumbar cancellous bone after osteoporosis-related happened; Qiang Gu Bao Po on the lumbar spine osteoporosis intervention effect may be related to reduced carbonic anhydrase, actin, crystal protein, 3- phosphoglycerate dehydrogenase, serum albumin, ATP synthase, myosin, the enolase expression.

从腰椎松质骨蛋白质组表达分析可知，肌动蛋白、角蛋白、烯醇化酶、ATP合酶、肌球蛋白的表达增强可能与去卵巢后腰椎松质骨骨质疏松的发生相关；强骨宝对腰椎骨质疏松的干预效应可能与下调碳酸酐酶、肌动蛋白、αB-晶体蛋白、3-磷酸甘油醛脱氢酶、血清白蛋白、ATP合酶、肌球蛋白、烯醇化酶的表达有关。

AIM: This experiment was designed to study the protective effects of maslinic acid, a novel glycogen phosphorylase inhibitor, on cerebral ischemia in mice.

目的：研究糖原磷酸化酶抑制剂-山楂酸对缺血性脑损伤的保护作用，初步探讨糖原磷酸化酶抑制剂的脑缺血保护机制。

Microphylla plantations are obvious, such effects and the contents of soil organic matter, N, P and K are gradually increased with the growth of C. microphylla plantations. Moreover, the activities of phosphomonoesterase, saccharase, protease, urease, dehydrogenase, polyphenol oxidase and nitrate reductase and the contents of edaphon C, N and P are significantly increased at soil depth of 0~30 cm, especially at soil depth of 0~10 cm.

随着小叶锦鸡儿固沙群落发育时间的增长，其综合防风性能提高，土壤有机质、N，P，K含量和生物活性逐渐得到改善。0~30 cm土层中磷酸单酯酶、蛋白酶、脲酶、蔗糖酶、硝酸还原酶、多酚氧化酶、脱氢酶的活性和土壤微生物生物量C，N和P含量均大幅度提高，其中0~10 cm土层增幅最大。

And also Hill reaction activity, unicycle photosynthetic phosphorylation activity and ATPase activity of flag leaves were positively correlated with photosynthetic rate.

而。且希尔反应活力、光合磷酸化活力及ATP酶活力与光合速率呈显著的正相关，因此希尔反应活力、光合磷酸化活力及ATP酶活力均可以作为高光效的的生理指标。

The level of cAMP in cell depends on adenyl cyclase and phosphodiesterase .

细胞内的环磷酸腺苷的水平取决于腺苷环化酶和磷酸二酯酶。

BADH cDNA (1785bp) included a 63 bp 5 "UTR, a 219 bp 3 "UTR and a 1503 bp ORF encoding a 500-amino-acid polypeptide which showed closely similar sequence identity to BADH from Chenopodiaceae.

将BADH的编码区推测的氨基酸在GenBank中进行分析，能找到磷酸果糖激酶、乙醛脱氢酶家族，谷胺酰磷酸还原酶等的保守区域。

Nineteen spots were changed significantly after FA treatment.Thirteen proteins were identified by peptide mass fingerprinting or peptide sequence analysis,including putative nucleoside diphosphate kinase,elongation factor 1-gamma,triosephosphate isomerase,60S acidic ribosomal protein P0,heat shock protein 75 kDa,similar to heat shock 70kD protein binding protein,annexin I,hypothetical protein FLJ34423,microtubule-actin crosslinking factor 1,lamin B2,ATP synthase alpha chain,mitochondrial precursor,proteasome subunit alpha type 6.These identified proteins involved in energy metabolism,translation and RNA processing,protein folding,redox regulation,cell structure and cell signaling.

双向凝胶电泳结果显示，甲醛刺激后19个蛋白斑点发生变化，肽指纹图谱及肽序列标签鉴定了其中13个蛋白斑点，已鉴定的蛋白包括二磷酸核苷酸激酶、延长因子1-γ，磷酸丙糖异构酶、60S酸性核糖体蛋白P0、75kDa热休克蛋白、70kD热休克蛋白样结合蛋白、钙依靠磷脂结合蛋白I、假想蛋白FLJ34423、微管-肌动蛋白交叉连接因子1、核纤层蛋白B2、ATP合成酶α链、蛋白酶体α亚基6，这些蛋白功能涉及转录调节、蛋白折叠、信号传导、能量代谢、细胞骨架等各个方面。

Thirteen proteins were identified by peptide mass fingerprinting or peptide sequence analysis,including putative nucleoside diphosphate kinase,elongation factor 1-gamma,triosephosphate isomerase,60S acidic ribosomal protein P0,heat shock protein 75 kDa,similar to heat shock 70kD protein binding protein,annexin I,hypothetical protein FLJ34423,microtubule-actin crosslinking factor 1,lamin B2,ATP synthase alpha chain,mitochondrial precursor,proteasome subunit alpha type 6.These identified proteins involved in energy metabolism,translation and RNA processing,protein folding,redox regulation,cell structure and cell signaling.

双向凝胶电泳结果显示，甲醛刺激后19个蛋白斑点发生变化，肽指纹图谱及肽序列标签鉴定了其中13个蛋白斑点，已鉴定的蛋白包括二磷酸核苷酸激酶、延长因子1-γ，磷酸丙糖异构酶、60S酸性核糖体蛋白P0、75kDa热休克蛋白、70kD热休克蛋白样结合蛋白、钙依赖磷脂结合蛋白I、假想蛋白FLJ34423、微管-肌动蛋白交叉连接因子1、核纤层蛋白B2、ATP合成酶α链、蛋白酶体α亚基6，这些蛋白功能涉及转录调节、蛋白折叠、信号传导、能量代谢、细胞骨架等各个方面。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新