磷酸酶

In the leaves, the mesophyll cells surrounding the vascular bundles contain the carbon dioxide-fixing enzyme phosphoenolpyruvate carboxylase in their cytoplasm.

在叶片中，叶肉细胞包围着维管束，叶肉细胞细胞质中所包含的磷酸烯醇式丙酮酸羧化酶，具有比核酮糖二磷酸羧化酶更高的的与二氧化碳的亲和力。

A variety of factors were comparatively studied with regard to their effects on the stability of phosphoglucomutasein Chinese cabbage.

首先对影响葡萄糖磷酸变位酶稳定性的因素进行了比较研究，结果发现，在所有被检测的因子中，磷酸根对酶的保护作用最强，而Tris－盐酸则效果极差。

The metabolism of these extreme microbes during the production of Maotai Liquor would further produce multiple enzymes of thermal stability such as amylase, protease, saccharifying enzyme, cellulose, glucase, xylanase, and each kind of dehydrase involved in redox reaction, and DNA polyase etc.

茅台酒酿造过程中极端酿酒微生物代谢产生多种热稳定性的酶，如淀粉酶、蛋白酶、糖化酶、纤维素酶、葡萄糖甘酶、木聚糖酶、参与氧化还原反应的各种脱氢酶、磷酸烯醇丙酮酸激酶及DNA聚合酶等。

For most piercing-sucking insects, they will secrete gell saliva and watery saliva when they feed on plants. The gell saliva will be secreted at the early stage of feeding to form salivary sheath, which is supposed to surround and protect the stylet. This saliva can help piercing-sucking insects to feed on plants directly and indirectly. Meanwhile, the watery saliva contains many components like pectnase, cellulose, polyphenol oxidase, peroxidase, alkaline phosphatase, sucrase, etc. These components can help piercing-sucking insects in plant tissue penetration, food digestion, detoxification of plant secondary substances and break-down of plant defence reaction.

对多数刺吸式昆虫而言，他们取食时会分泌胶状和水状两种唾液，其中胶状唾液会在取食早期分泌形成唾液鞘来围绕并保护口针，通过直接和间接的作用来帮助取食；而水状唾液中则包含了果胶酶、纤维素酶、多酚氧化酶、过氧化物酶、碱性磷酸酯酶、蔗糖酶等组分，来帮助刺吸式昆虫对植物穿刺、消化食物、解毒次生物质并破坏植物的防御反应。

Glyceraldehyde 3-phosphoate is converted to 1,3-bisphosphoglycerate(by glyceralbehyde 3-phosphatedehydrogenase) which reacs with ADP to give 3-phosphoglycerate and ATP (catelyzed by phosphoglycerate kinase).

果糖1，6二磷酸裂解为甘油醛3－磷酸和二羟丙酮磷酸；则两个丙糖被丙糖磷酸化异构酶相互转化。

The results indicated that Fructose 1,6-bisphosphate aldolase,succinate dehydrogenase ,and hexokinase were very sensitive to temperatures .Citrate-synthase was similar to that of mesophiles in temperature properties .

结果表明Y18细胞中1，6-二磷酸果糖醛缩酶，琥珀酸脱氢酶和己糖激酶对温度很敏感，柠檬酸合成酶的温度效应类似于中温酶，α-酮戊二酸脱氢酶和异柠檬酸脱氢酶存在不同温度特性的同功酶。

In April 2004, 30 Boleophthalmus pectinirostris samples were collected from the lagune in Xiepu Villege, Zhenhai Town, Ningbo City, Zhejiang Province. Polyarylamide gel electrophoresis was used to detect the expression of isozymes in liver tissue. LDH, FDH, GDH, GCDH, G6PDH, MDH, ME, SOD, CAT and EST were analyzed and the biochemical genetic results showed that, among the 10 isozymes in this paper, 9 of which obtained clear enzyme patterns except for CAT.

于2004年4月，在浙江省宁波市镇海区澥浦滩涂采集野生大弹涂鱼30尾，采用聚丙烯酰胺凝胶电泳方法，分析了乳酸脱氢酶、甲酸脱氢酶、谷氨酸脱氢酶、D-葡糖脱氢酶、葡萄糖－6-磷酸脱氢酶(G6PDH)、苹果酸脱氢酶、苹果酸酶、超氧化物歧化酶、过氧化氢酶和酯酶等10种酶在大弹涂鱼肝脏组织中的表达，并对同工酶表型进行了生化遗传分析。

From the femur bone density analysis of protein expression, we can see that light chain lactoferrin, annexin decreased and the expression of enolase, ATP synthase, acetyl-coenzyme A reductase, the expression of troponin, may be related to ovariectomized femoral bone density in osteoporosis-related happened; strong bone of the femoral osteoporosis Po intervention effect may be related to reduced enolase, ATP synthase, troponin, CK-MB, acetyl phosphoglycerate enzyme, the expression of myosin, and the increase in lactoferrin light chain, pyruvate kinase isoenzyme, protein expression crown.

从股骨密质骨蛋白质组表达分析可知，乳铁蛋白轻链、膜联蛋白A3的表达下降及烯醇化酶、ATP合酶、乙酰辅酶A还原酶、肌钙蛋白的表达增强，可能与去卵巢后股骨密质骨骨质疏松的发生相关；强骨宝对股骨骨质疏松的干预效应可能与下调烯醇化酶、ATP合酶、肌钙蛋白、肌酸激酶同工酶、磷酸甘油酸变味酶、肌球蛋白的表达，和上调乳铁蛋白轻链、丙酮酸激酶同工酶、冠蛋白的表达有关。

In addition, SW480 and HCT116 cells were treated with deoxycholic acid for 15, 30, 45, 60, 90 and 120 minutes. It was found that the deoxycholic acid treated cells showed an elevation of ATP activity by 12.35 %, 20.08 %, 29.69 %, 33.56 %, 45.85 %, 54.32 %, respectively. While the HCT116 treated cells, showed an elevation of ATP activity by 3.69 %, 5.59 %, 8.52 %, 12.26 %, 16.52 %, 19.71%, respectively.From the experiments conducted using the cell lines described above, it was further confirmed that genes CYP7A1 (cytochrome P450, family 7, subfamily A, polypeptide 1), AKR1D1 (aldo-keto reductase family 1, member D1), ALDH1A1 (aldehyde dehydrogenase 1 family, member A1), BAAT (bile acid Coenzyme A: amino acid N-acyltransferase), ABCA1 (ATP-binding cassette, sub-family A, member 1), APOC3 (apolipoprotein C-III), LIPA, LIPE (lipase, hormone-sensitive), PTGER2 (prostaglandin E receptor 2) and PTGS2 (prostaglandin-endoperoxide synthase 2) were up-regulated.

此外，为了证实其脂肪代谢产物胆酸对细胞癌化的影响，亦使用SW480和HCT116细胞株，经由脱氧胆酸在15分钟、30分钟、45分钟、60分钟、90分钟和120分钟处理后，其细胞增生比率分别有12.35%，20.08%，29.69%，33.56%，45.85%和54.32%(p.05)以及3.69%，5.59%，8.52%，12.26%，16.52%和19.71%(p.05)上升情形，并从这些细胞株进一步证实CYP7A1(细胞色素P450-7A1)、AKR1D1(醇醛酮还原酶-1-D1)、ALDH1A1(醛脱氢酶-1-A1)、BAAT(胆酸辅酶A：氨基酸N-醯基转移酶)、ABCA1(三磷酸腺苷结合盒转运体A1)、APOC3、LIPA、LIPE(脂肪酶/激素敏感脂肪酶)、PTGER2(前列腺素E受体2)和PTGS2(前列腺素内过氧化物合成酶2)等基因表现皆有呈现向上调控的情形。

The identification of protein were performed by searching the mass spectrometry data from the NCBInr database using the software of Sequest Bioworks3. 2. The results indicated that over 25 protein components including acid and basis phospholipase A2, nerve growth factor, presynaptic neurotoxin, hemorrhagic factor, thrombin-like enzyme, serine protease, glycogen synthase, metalloproteinase, maturase, L-amino acid oxidase, plasminogen activator, adhesion, cysteine-rich secretory protein, disintegrin, fibronectin-binding protein, fibrinogen clotting inhibitor A chain, vascular apoptosis-inducing protein 1, pyranose 2-oxidase, carbamoyl-phosphate synthase, as well as the homology proteins of snake venom ablomin, ablomin, catrin, mucrofirase, pallabin, salmosin and triflin homologues were identified from the GSSV.

从GSSV中鉴定出超过25种蛋白组分，主要包括：酸性磷脂酶A1、碱性磷脂酶A2、神经生长因子、突触前神经毒素、出血因子、类凝血酶、丝氨酸蛋白酶、糖原合酶、金属蛋白酶、成熟酶、L-氨基酸氧化酶、纤溶酶源激活物、外源凝集素、富含半胱氨酸分泌酶、去整合素、纤连蛋白结合蛋白、纤维蛋白原凝固抑制因子A、血管凋亡诱导蛋白1、氨甲酰基磷酸合成酶、吡喃糖－2-氧化酶以及ablomin， catrin， mucrofirase， pallabin， salmosin和triflin的同源蛋白。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新