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Phytase was a kind of enzyme for hydrolyzing phytate to release myoinositol and inorganic phosphate, which would promote the utilization of phosphate, reduce the drainage of phosphate in the dejecta and reduce the environmental pollution.

植酸酶是催化植酸及植酸盐水解成肌醇与磷酸的一类酶的总称。植酸酶添加在饲料中,会将其中的植酸水解成肌醇和磷酸,从而提高了磷的利用率,降低了粪便中磷的排放量,减少了植酸磷对环境的污染。

Glucose-6-phosphate dehydrogenase (G6PD, E. C. 1. 1. 1. 49) is a house-keeping enzyme in all cells, and also is a key enzyme in the pentose phosphate pathway. G6PD deficiency, affecting 200~400 million people in the world, can cause chronic non-spherocytic hemolytic anemia, favism, drug-induced hemolytic anemia, neonatal jaundice and several infectious diseases. Its inheritance mode is an X-linked incomplete dominance.

葡萄糖-6-磷酸脱氢酶(Glucose-6-Phosphate Dehydrogenase,G6PD,E.C.1.1.1.49)是存在于所有细胞中的一种看家酶,也是磷酸戊糖途径的关键酶。G6PD缺乏症累及全球2~4亿人,引起慢性非球形细胞溶血性贫血、蚕豆病、药物性溶血、新生儿黄疸及某些感染性溶血,其遗传方式是X连锁不完全显性方式。

Results showed that yield of TCA-N in phosphorylated fish protein hydrolysate approached to that of the control sample and phosphatization had little effect on hydrolysis.

对低值鱼蛋白磷酸化改性,并进行体外酶解,研究了磷酸化鱼蛋白的酶解特性(酶解物氨态氮得率、TCA可溶性肽得率及肽分子量分布)。

In muscle tissue, the rate of conversion of glycogen to glucose 6-phosphate is determined by the ratio of phosphorylase a to phosphorylase b.

在肌肉组织中,糖原转化成6-磷酸葡萄糖的速率由磷酸化酶a和磷酸化酶b的比例决定。

This method is more simple and inexpensive than screening cDNA library, and can be easily adapted to clone other genes. An Amorpha fruticosa cDNA clone encoding UDP-glucose pyrophosphorylase, a key enzyme producing UDP-glucose in the synthesis of sucrose and cellulose, was cloned by using this method.

本论文利用此方法首次从紫穗槐中克隆了与纤维素合成前体----尿苷二磷酸葡萄糖的代谢有关的尿苷二磷酸葡萄糖焦磷酸化酶基因和与木质素合成相关的4-香豆酸:CoA连接酶(4CL)基因的全长cDNA序列,并由此推测出相应的氨基酸序列。

Rubisco can also act as an oxygenase breaking down ribulose bisphosphate in the presence of oxygen to glycerate 3-phosphate and phosphoglycolic aid. This reaction forms the basis of PHOTOREESPIRATION.

此酶在有氧的情况下可为氧化酶,分解核酮糖-1,5-二磷酸生产3-磷酸甘油和磷酸乙醇酸,这一反应是光呼吸的基础。

Ribulose bisphotosphate carboxylase (RuBP carboxylase ; rubisco) An enzyme that catalyzes the carboxylation of ribulose biphosphate to two molecules of glycerate 3-phosphate, the carbon dioxide-fixing first step in the Calvin cycle of PHOTOSYNTHESIS.

1,5-二磷酸核酮糖羧化酶:催化核酮糖-1,5-二磷酸成为两分子3-磷酸甘油酸的酶,这是光合作用卡尔文循环中,二氧化碳固定的第一位反应。

Rats in control group and model group received 0.9% sodium chloride solution 10 mL/kg. Rats in compound danshen dripping pills group received 0.75% compound danshen dripping pills 10 mL/kg. Rats in Xintongzhi drop pill high dose group and Xintongzhi drop pill low dose group received 3.6%, 1.8% Xintongzhi drop pill 10 mL/kg, respectively. After 7 days except control group, myocardial ischemia models were established by ranine vein of hypophysin in other four groups. The changes of lactate dehydrogenase,α-Hydroxybutyrate Dehydrogenase, creatine kinase, creatine kinase MB and ECG were recorded in each groups.

正常组和模型组予0.9%氯化钠注射液,复方丹参滴丸对照组予0.75%复方丹参滴丸水溶液,心痛治滴丸高剂量组、心痛治滴丸低剂量组分别给予3.6%、1.8%的心痛治滴丸水溶液,各组均10 mL/kg,每日1次灌胃。7日后除正常组外,其余4组均采用垂体后叶素0.5 U/kg舌下静脉注射制备大鼠急性心肌缺血模型,并检测各组乳酸脱氢酶、α-羟丁酸酶、磷酸肌酸激酶及磷酸肌酸激酶同工酶和心电图的改变。

Result At 5 and 25 mgkg^(-1), the total respiratory rate had a similar trend with tricarboxylic acid cycle and pentose phosphate pathway, T1, and T2 were higher than the control on the 1st day and then declined three days late but they had a recover on the last day. Embden-Meyer-hot-Parnas pathway had a declined trend forever. The respiratory rate of the total and three pathways were decreased under 125 mgkg^(-1). The enzyme activity of pyrophosphate-dependent phosphofructokinase, glucose-6-phosphate dehydrogenase (G-6-PDH) and malate dehydrogenase were similar to relative respiratory pathway.

测定期内,5mgkg^(-1)土、25mgkg^(-1)土浓度处理使基础呼吸速率和三羧酸循环、磷酸戊糖途径的呼吸速率均表现初期上升随后(第3天)下降但末期有所恢复的趋势,而糖酵解途径的呼吸速率则持续下降在末期小幅回升;125mgkg^(-1)土浓度处理后基础呼吸速率及3条途径呼吸速率均呈下降趋势;磷酸果糖激酶、苹果酸脱氢酶和葡萄糖-6-磷酸脱氢酶(G-6-PDH)活性变化趋势分别与其对应途径呼吸速率变化相似。

However, fully consideration of special structure and physiological function of CRP is critical to investigate whether CRP provide protect effect or pathogenic effect in the progression of atherosclerosis. CRP is a member of pentrxins family, which consists of five identical, noncovalently associated 23-kDa protomers arranged symmetrically around a central pore with a 102 A external diameter. Each protomer has a recognition face with a phosphocholine binding site consisting of two coordinated calcium ions adjacent to a hydrophobic pocket. The opposite face of the pentamer is the effector face, where complement Clq binds and Fc receptors are presumed to bind. A cleft extends from the center of the protomer to the central pore of the pentamer. Both faces ofpentamer form a special bi-phase structure: one face is the ligand recognition phase, which can recognize apoptosis cells and enzyme modified low density lipoprotein in which PCh is exposed.

CRP是一种五聚体蛋白,外径为102 A,由5个相同的单体以非共价键结合,形成双面的环形结构,每个单体的配体识别位点或受体结合位点分别位于五聚体两个平面上,分别组成配体识别相和效应器相,从而构成特殊的二相性结构:一面为配体识别相,含有磷酸胆碱(phosphorycholine,PCh)结合位点,能够使CRP识别在病理条件下暴露出PCh的凋亡细胞、酶修饰低密度脂蛋白(enzyme modified low density lipoprotein,E-LDL)等。E-LDL是天然低密度脂蛋白(native Low density lipoprotein,N-LDL)在多种蛋白酶,如胰蛋白酶、神经氨酸酶、胆固醇酯酶等作用下的代谢产物,其主要特点是结构发生改变(N-LDL大小均匀,平均直径250±30 A,E-LDL大小差异极大,直径为100-2000 A),暴露磷酸胆碱位点,能够被CRP识别;另一面为效应器相,能够与巨噬细胞、单核细胞表面受体FcγRⅡa结合,介导巨噬细胞、单核细胞识别及吞噬凋亡、坏死细胞作用。

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