磷酸酶

MAIN OUTCOME MEASURES: Results of alkaline phosphatase staining and alizarin red staining; Results from quantitative analysis of alkaline phosphatase and osteocalcin expression.

主要观察指标：碱性磷酸酶染色与茜素红染色结果，碱性磷酸酶和骨钙素定量分析结果。

Alkaline phosphatase staining: ES cell clones were stained dark blue, showing alkaline phosphatase activity.

碱性磷酸酶染色：ES细胞克隆被染成深蓝色，说明细胞显示碱性磷酸酶活性。

Cells were cultured for 6 days after the intervention, cell content of alkaline phosphatase decreased significantly over the blank control group, each group of Chinese Medicine groups compared with the control group increased in varying degrees, and with the increased dose alkaline phosphatase content moving in upward trend.

各组细胞干预6天后，对照组细胞内的碱性磷酸酶含量较空白组明显下降，而中药各组较对照组均有不同程度升高，且随着药量的加大碱性磷酸酶含量呈上升趋势。

The Effects of ED -71 on proliferation and differentiation were detected by MTT,.cell cycle, alkaline phosphatase activity and level of osteocalcin.

采用细胞形态学，碱性磷酸酶染色，培养细胞匀浆上清测定单位蛋白下的碱性磷酸酶活性，培养基中骨钙素含量分析及原位杂交方法检测ED-71对OS-732人成骨样细胞分化的影响。

A complex sample containing BMP and sodium fluoride was implanted under the abdominal skin of young male Wistar rats. The histiological characteristcs, and activities of alkine phosphatase and acid phosphatase of the implantan were examined to study the effects of fluoride on biological activities of BMP.

在纯化的BMP中加入氟化物再植入大鼠皮下，通过比较植入物在不同植入时间的组织学变化，碱性磷酸酶、酸性磷酸酶活性及钙含量来反映BMP的生物活性。

Acid phosphatase, alkaline phosphatase, and superoxide dismutase were found in the serum, blood cells and liver extract of Chlamys farreri . The activities of ACP and ALP were highest in the liver.

在栉孔扇贝的血清、血细胞和肝脏提取液中均发现有酸性磷酸酶、碱性磷酸酶和超氧化物歧化酶活性，其中，ACP和ALP在肝脏中的活性最高。

The effects of FPL andits three ingredients on growth of ovary,on micro-and ultra-structure andAlkaline phosphatase activity of endometrium,on structure andkeratinization rate of exfoliated cells in vaginal epithelium in mice,onmicrocirculation in rat and on uterine kinesis in rabbit were respectivelyobserved by the techniques of light and electron microscopes,histochemistry,microcirculation and organ cinetography.The results have confirmed themechanism of FPL for the sterility caused by ovary standstill and persistentcorpus luteum to be as follows:(1) a weak estrogen-like role which canprompt development of ovary,proliferation and secretion of endometrium andvaginal epithelium and cornification of exfoliated cells in vaginal epithelium;(2)enhancing AKP's activity of endometrium;(3)improving microcirculation;(4) exciting uterus.

分别采用光学显微镜和电子显微镜技术、组织化学技术、微循环技术和器官运动描记等现代医学实验技术，观察了促孕液对小白鼠的卵巢发育、子宫内膜的显微和超微结构和碱性磷酸酶活性、阴道上皮结构和脱落细胞的角化率、对大白鼠的微循环和对家兔的子宫运动的影响，结果证实了促孕液治疗卵巢静止、持久黄体的机理：(1)微弱的雌激素样作用，能刺激卵泡发育，促进子宫内膜和阴道上皮增生、分泌和阴道上皮脱落细胞角化；(2)提高子宫内膜碱性磷酸酶的活性；(3)改善微循环；(4)促进子宫运动。

33 Holstein cows and 29 Tibet-sheep were chosen to study the alkaline phosphatase activity and its enzyme retention in different period of pregnancy with disodium phenyl phosphate method.

方法］采用磷酸苯二钠法，对33头荷斯坦奶牛和29只藏系绵羊不同怀孕期血清碱性磷酸酶活性及其酶保存率进行了测定。

We investigated the effect of combined application of maize straw, bean haulm, wheat straw and weathered coal on the activity of alkaline phosphate enzyme in calcareous cinnamon soil by means of regression design of 416A-optimization.

本研究采用四因数二次回归 4 1 6A—最优设计方法，通过裸土盆栽试验，在施用少量N、P肥的基础上，研究了玉米秆、豆秆、麦秆和风化煤四种有机物料配合施用对石灰性褐土中碱性磷酸酶活性的影响，建立了上述四个因子与土壤碱性磷酸酶活性之间的数学模型，并分别进行了试验因子的主效应和互作效应的分析

These changes were prevented by genistein (a protein tyrosine kinase inhibitor) and antioxidant N-acetyl-L-cysteine, but promoted by sodium orthovanadate (a protein phosphatase inhibitor), which were administered to the SD rats 20 min before ischemia.

进一步的研究表明，缺血前20 min腹腔注射给药，然后缺血30 min，发现蛋白酪氨酸激酶抑制剂染料木黄酮和抗氧化剂N-乙酰半胱氨酸能显著地抑制核内STAT3的磷酸化水平及DNA结合活性的增加(磷酸化水平从2.3和2.5倍分别降为1.2和1.4倍， DNA结合活性则从2.8和3.7倍分别降为1.1和1.5倍)，而蛋白酪氨酸磷酸酶抑制剂矾酸钠则能明显地促进他们的增高(磷酸化水平从2.0倍增到3.4倍， DNA结合活性从3.1倍增为5.1倍)。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。