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At the end of the culture, PBMCs cultured on coverslips were fixed and stained for tartrate resistant acid phosphatase, a marker of osteoclast.

对培养终末细胞作组织化学染色,检测破骨细胞特征性标志物抗酒石酸磷酸酶的表达;并以象牙磨片上虫蚀样骨吸收陷窝的形成为指标检测其生物学活性。

METHODS: The rhBMP-2,expressed in Escherichia coli, was washed by Triton X- 100 and further purified by DEAE chromatography.The inclusion bodies were resolved in 8 mol/L urea, and were refolded and dimerized in the redox systems (reduced and oxidized glutathione). Finally, a one - step purification procedure based on the heparin affinity chromatography was implemented. The biological activity of purified rhBMP - 2 were tested by induction of the alkaline phosphatase activity in C2C12 cells.

在大肠杆菌中通过温度诱导表达重组人骨形成蛋白-2经过Triton X-100清洗之后,又通过DEAE离子交换层析纯化包涵体,包涵体在8 mol/L尿素变性溶解,在氧化-还原(还原型和氧化型谷胱甘肽)复性系统中,通过简单的稀释复性,通过肝素亲和层析一步纯化法纯化重组人骨形成蛋白-2,最后通过诱导C2C12细胞产生碱性磷酸酶检测重组人骨形成蛋白-2活性。

The effects of effectors such as multiple metal ions and organic solvents on ALP in viscera of rice-field eel.

方法]研究多种金属离子、有机溶剂等效应物对黄鳝内脏碱性磷酸酶活性的影响。

Results Bile flow, serum levels of total bile acid and gamma glutamyl transpe...

而给予SAMe的Ⅲ、Ⅴ组胆汁流升高,血清总胆酸和γ GT水平明显降低,肝脏病理结果正常、毛细胆管不扩张、无胆栓;临床 16例患者给药前表现为胆汁瘀积和肝功能损害,给予SAMe 3周后血总胆红素、碱性磷酸酶、γ GT、谷丙转氨酶、谷草转氨酶均较给药前的异常水平有显著下降,第 4周恢复至正常水平。

Their activities, heat-proof, acid-proof and alkaline-proof of the two enzymes were inhibited with increasing TBT concentrations.The effect of TBT on the phagocytic activity of oyster haemocytes was studied in vitro and in vivo.

从未受污染的牡蛎个体中分离的碱性磷酸酶和超氧化物歧化酶的活性、耐热性、耐酸碱性均随TBT浓度的增大而降低。

Obstructive jaundice was induced in the mice in the experimental group by common bile duct ligation. The level of the common bile duct diameter, WBC, LYM MID, LYM%, MID% and ALT, AST, TBIL, DBIL, IBIL, ALP and CHOL were measured 12 h and 1, 2, 3, 4, 5, and 7 days after the ligation.

于实验的第12 h,1、2、3、4、5、7 d动态观察胆总管直径及白细胞总数、淋巴细胞总数、中间细胞总数及淋巴细胞总数百分数和中间细胞总数百分数,测血清总胆固醇、丙氨酸氨基转氨酶、天冬氨酸氨基转氨酶、碱性磷酸酶、总胆红素、直接胆红素及间接胆红素值。

Besides, they play important roles in the regulation of mitogenic signal transduction and cell cycle control in response to extracellular stimuli.

此外,它们还在有丝分裂原激活蛋白磷酸酶信号途径中起重要生理功能。

The gather of like Embryonic Stem cell was got and cultured from the morulas.

从得到的桑椹胚分离获得了类胚胎干细胞集落,碱性磷酸酶染色,集落呈阳性。

The method has thyroid transcribe factor to specimen of 12 SHL tissue - antigen of 1(TTF-1), epithelial film , CK7, CK20, dash forward the albumen that touch bubble , undee albumen , flowing flesh actin , placental alkalescent phosphoric acid is enzymatic , be addicted to bead albumen, among them observation of 10 lens that make report.

方法对12例SHL组织标本进行甲状腺转录因子-1(TTF-1)、上皮膜抗原、CK7、CK20、突触泡蛋白、波形蛋白、平滑肌肌动蛋白、胎盘碱性磷酸酶、嗜铬粒蛋白、CD34的SP免疫组织化学法标记,其中10例做电镜观察。

RESULTS:① Both induced osteoblasts and MSCs exhibited ALP and calcium node stainings positive.

结果:①诱导成骨分化的骨髓基质干细胞分泌的碱性磷酸酶及钙结节染色均呈阳性。

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