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Osthole and TCFC reduced the bone lose by decreasing the osteoclast formation, its TRAP activity, and osteoclastic bone resorption.

蛇床子素和蛇床子总香豆素是通过抑制破骨细胞的形成、抗酒石酸酸性磷酸酶的活性和骨吸收作用减少骨质丢失的。

Methods Osteogenetic culture medium containing different concentrations of atorvastatin 10^(-6, 10^(-7), 10^(-8)mol/L and control were added in the murine bone marrow cell subculture. The proliferation of the stromal cells was observed, and alkaline phosphatase staining and examination were done to check the differentiation ability of bone marrow cells. Alizarin red staining was performed to evaluate the mineralization of the osteoblasts in culture.

分别将10^(-8)、10^(-7)、10^(-6)mol/L的阿托伐他汀和空白药物加入传代培养的小鼠骨髓基质细胞,通过细胞形态学观察、细胞增殖率检测、碱性磷酸酶检测和钙结节染色,比较药物在成骨细胞分化过程中对细胞增殖和分化的影响。

MAIN OUTCOME MEASURES: The morphology of BMSCs was observed by the inverted phase contrast microscope. Expression of BMSC surface antigens was detected by flow cytometer. Osteogenous potential was assessed by alkaline phosphatase staining. Adipogenic potential was evaluated by Oil red O staining. Chondrogenic potential was assessed by toluidine blue staining.

主要观察指标:倒置相差显微镜下观察骨髓间充质干细胞的生长状态,流式细胞仪检测细胞表面标志物的表达,采用碱性磷酸酶染色鉴定成骨能力,以油红O染色鉴定成脂能力,以甲苯胺蓝染色鉴定成软骨能力。

After osteoplastic induction, peripheral blood MSCs had strongly positive reactions for alkaline phosphatase staining, total collagen staining, alizarin red staining.

外周血间充质干细胞成骨诱导后,碱性磷酸酶染色、总胶原染色及茜素红染色均呈强阳性;成脂诱导后油红染色呈阳性,有一定数量的脂滴形成。

In order to understand the mechanisms of livial in prevetion of bone loss in patients with premeture ovarin failure.12 cases pstients were treated with livial for two months.Then fasting urinary calcium creatinine ratio、 hydroxyproline creatinine natio、 Serum akaline phosphotase、 estradio(E2)、 Calcitonin、 bone gla-protein and interleukin-6(IL-6) were determined for these patients before and after treatment.

本文通过给12例卵巢早衰患者利维爱用药2个月,并于给药前后分别测定空腹尿钙与肌酐、羟脯氨酸与肌酐比值以及血清碱性磷酸酶、雌二醇(E2)、骨钙素、降钙素和白细胞介素-6(IL-6)的水平,以探讨Livial防止卵巢早衰患者骨量丢失的作用机制。

Results According to the treatment group, The total protein、albumin、globulin、the former albumin、serum glucose are higher than that of the control group; the Immunoglobulin (IgG、IgA、 IgM) are significantly higher than that of the control group, and the urea is significantly lower than that of the control group. Other indicators: total bilirubin、glutamic pyruvic transaminase、glutamic oxalacetic transaminase、alkaline phosphatase、creatinine、hemoglobin and the red blood cell count have no significant difference between the two groups.

结果 治疗组总蛋白、白蛋白、球蛋白、前白蛋白、血糖比对照组高;尿素比对照组明显减低;免疫球蛋白(IgG、IgA、IgM)比对照组明显升高;总胆红素、谷丙转氨酶、谷草转氨酶、碱性磷酸酶、肌酐、血红蛋白、红细胞计数等项,两组无明显改变。

Methods Making a pediculated skin graft on dog's upper lip ,and then got it into oral cavity via the "skin tunnel", laying it on the maxillary bone surface (the same size of the alveolar mucosa has been removed),and suturing the graft edges with its surrouding alveolar mucosa.

目的 观察钛种植体与口腔内非牙龈黏膜软组织形成的界面情况;同时观察种植体与周围软组织间隙&龈沟液&的量及其中的天冬氨酸转氨酶和碱性磷酸酶的水平变化与软组织周围炎症的关系。

Expression of collagen I,II and alkaline phos┐phatase in osteogenesis by rhBMP┐2in vivo

rhBMP┐2在体内诱导成骨中I,II型胶原及碱性磷酸酶的表达

The bone mineral density and Z score in distal 1/3 radius were measured with dual energy X-ray absorptiometry in 185 he modialysis patients, meanwhile serum parathyroid hormone, alkaline phos phatase and hand X-rays were detected.

采用双能X线骨密度测定法检测185例慢性肾衰血透患者的桡骨骨密度,计算其Z值,同时检测其血清甲状旁腺素,碱性磷酸酶和指骨X照片。

Osteoblasts were detected by alkaline phosphatese and calcium salt staining. The expression of osteopontin was measured by Western blotting.

成骨细胞鉴定采用组织化学方法行碱性磷酸酶和钙化斑块染色同时应用Western blotting法测定骨桥蛋白表达。

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