磷酸酶

The plasmalemma of cells within meristematic regions was observed to possess invaginations in cultured callus of Stevia rebaudiana under differentiation.

对在分化条件下的甜菊愈伤组织分生区域细胞的质膜内陷进行了超微结构和酸性磷酸酶细胞化学研究。

The cells were identified by invert phase contrast microscopy and alkaline phosphatase staining and mineralized nodules staining.The activity distribution of osteoblasts was determined by microtitration during a culture cycle.

通过倒置相差显微镜观察，并用碱性磷酸酶和矿化结节染色鉴定，利用微量滴定法测定一个培养周期的成骨细胞活性分布，探讨一种经济、高效的原代成骨细胞培养方法。

Our study showed that about 3% mononucleated cells of human peripheral blood could be induced to osteoclast-like cells which have the ability of bone resorption and show TRAP-positive when coculture with UMR106 in the presence of 1,25 dihydroxyvitamin D3, macrophage-colony stimulating factor and dexamethasone.

在1，25_2D_3、地塞米松和M-CSF存在下，人外周血单个核细胞与UMR106细胞共培养，约3%的单个核细胞可被诱导成为具有骨吸收能力且抗酒石酸酸性磷酸酶染色阳性的破骨细胞样细胞。

Results: The TRAP positive multinuclear cells were induced from rat marrow mononuclear cells after inoculating for 5 clays. RT-PCR analysis showed that ASIC1, ASIC2 and ASIC3 mRNA were present in the osteoclasts, and the corresponding proteins expressions were detected.

结果：诱导5d后可见抗酒石酸盐酸性磷酸酶阳性多核细胞出现，大鼠破骨细胞在mRNA和蛋白水平均可检测到ASIC1、ASIC2和ASIC3基因的表达产物。

Methods Marrow cells from NH mouse were harvested and cultured in α-MEM with 10% fetal bovine serum.The appearance of tartrate-resistant acid phosphatase-staining multinuclear cells and formation of bone resorption pits were measured after 3,6,9 and 12 day exposure to various concentrations of 1,25-2D3 in culture. Results 1,25-2D3 (concentration higher than 10-9mol/L) could induce recruitment of OLCs and their bone resorption activity in a dose-dependent manner.

收集NH小鼠骨髓细胞于含10%胎牛血清的α-MEM培养基中行体外培养，设置不同的1，25-2D3浓度组和给药时间组，并于培养第3、6、9、12天观察记录抗酒石酸酸性磷酸酶(tartrate-resistant acid phosphatase，TRAP)阳性多核巨细胞［或破骨细胞样细胞(osteroblast-like cell，OLC)］以及骨磨片上骨吸收陷窝的数目。

AIM: To examine the role of matrix metalloproteinases expressed by the tartrate-resistant acid phosphatase-positive mononuclear and multinucleated cells in articular cartilage damage.

目的：观察在胶原诱导的C57BL/6小鼠类风湿性关节炎模型中，基质金属蛋白酶-2、9在耐酒石酸盐酸性磷酸酶阳性的单个核及多核细胞中的表达及其在关节软骨损伤中的作用。

Methods: M-CSF-dependent bone marrow cells were isolated from 5-6 weeks old mice, and cultured in the presence of MCSF (25μg/L) with different concentrations of TNF-α(0, 1, 10, 100 μg/L) for 5 days, the formation of TRAP multinucleated cells was observed. These cells were also cultured in the presence of both RANKL (30 μg/L) and M-CSF (25 μg/L) with or without 10 μg/L TNF-α for4, 5, 6 and 9 days. The number of TRAP multinucleated cells and resorption pits on dentine slices were counted under light microscope.

选用小鼠巨噬细胞集落刺激因子依赖性非附着性骨髓细胞，在含有25μg/L M-CSF和0，1，10，100μg/L TNF-α的α-MEM培养液中培养5d后，观察抗酒石酸酸性磷酸酶染色阳性多核细胞的形成；细胞在含有25μg/L M-CSF和30μg/L sRANKL的α-MEM培养液中进行培养，比较加入和不加入10μg/L TNF-α培养4、5、6和9d后，所形成的TRAP多核细胞的数目和骨吸收面积。

Methods: M-CSF-dependent bone marrow cells were isolated from 5-6 weeks old mice, and cultured in the presence of M-CSF (25 μg/L) with different concentrations of TNF-α(0, 1, 10, 100 μg/L) for 5 days, the formation of TRAP multinucleated cells was observed.

选用小鼠巨噬细胞集落刺激因子依赖性非附着性骨髓细胞，在含有25μg/LM-CSF和0，1，10，100μg/LTNF-α的α-MEM培养液中培养5d后，观察抗酒石酸酸性磷酸酶染色阳性多核细胞的形成；细胞在含有25μg/LM-CSF和30μg/LsRANKL的α-MEM培养液中进行培养，比较加入和不加入10μg/LTNF-α培养4、5、6和9d后，所形成的TRAP多核细胞的数目和骨吸收面积。

The influences of some metal ions in seawater treated by Cephlex 100 on metamorphosis, growth and activities of alkaline phosphatase and ATPase in mysis and postlarvae of Chinese prawn Penaeus chinensis were studied.

向经过螯合剂Cephlex 10 0处理的海水中添加金属离子，进行培养中国对虾糠虾幼体和仔虾幼体，观察金属离子对变态发育及其体内碱性磷酸酶和ATPase活性的影响。

Serum indicators-urea nitrogen, cholesterol total, alkaline phosphatase manifestly were heightened and some animals' hepatic cells, nephric tubules and mucosa emerged differently damage at histomorphology by giving crude high dose after 2 months.

连续给药2个月，可见生品的高剂量组的血清生化指标的尿素氮、总胆固醇和碱性磷酸酶明显升高，组织形态学检查部分动物的肝细胞、肾小管和胃黏膜均产生程度不同的损伤。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。