磷酸酶

Thus, it is important to enucleate the catalytic centre and mechanism of transferrins as a phosphatase, which may have a potential significant in the protein phosphorylation process.

据文献报导，传铁蛋白家族中的某些乳传铁蛋白和铁结合蛋白具有磷酸酶的功能，因此，研究传铁蛋白磷酸酶学功能位点和作用机制并进一步确定它们在磷酸化过程的作用具有重要意义。

Purple acid phosphatases catalyze the hydrolysis of a wide range of natural and synthetic orthophosphate esters and anhydrides including ATP, pyrophosphate and phosphotyrosyl proteins.

与其他磷酸酶不同，紫色酸性磷酸酶中含有Fe-M双金属原子中心而使该酶具有天然的紫色或粉色，它也因此受到科学家们的广泛重视。

This may be because that the metal ions in calf intestine AKP is more close to the surface of enzyme molecule than in Ulva pertusa kjellm , EDTA can exerted inhibitory effect on the AKP from calf intestine more easily and quickly.

这可能是由于小牛肠碱性磷酸酶活性位点的金属离子相对于孔石菇碱性磷酸酶的活性伍点来说突出在酶大分子的表面，EDTA容易与之发生络合作用，从而快速对小牛肠AKP产生抑制作用。

The authers fetched the embryo calvarial peristeum tissue, got human osteoblast by enzyme-assimilating methods and tissue-block culture methods. We observed the morphological change, growth feature and osteogentic capability, of osteoblast during culture in vitro with phase contrast invert microscope, drew the growth curre and identified the cells by alkaline phosphatase dye. At same time, the morphology and bioactivity of 3-5th-generation osbeoblast and anabiotic cells was studied comparatively. 2. titanium particles were examined by scanning electron and the size was determined by semi-automated image analysis. The 3-5 th gereration of human osteoblast were cultured in medium with different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml). Cell growth and proliferation was detected by MTT method after 2、4、6 days that particles were added into medium and ALP activity was measured by kit after 4、7、10 days respectively. 3. With above same methods,the 3-5th generation of human osteoblasts were cultured for 3、6、9days after different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml) were added into the medium and OPG gene expression was quantified by RT-PCR.

1、取人胚胎颅骨骨膜，采有用酶消化法和组织培养法获取成骨细胞体外培养并传代，观察细胞形态，生物特点及成骨特性，并绘制生长曲线同时碱性磷酸酶染色鉴定成骨细胞以及比较冻存前3-5 代与冻存后成骨细胞的特点。2、电镜下观察钛合金颗粒的形态并测量其粒径，将不同浓度的钛合金颗粒(1mg/ml，0.1mg/ml，0.01mg/ml)与成骨细胞共同培养，分别于第2、4、6 天用MTT 法测量细胞增殖情况及4、7、10天用试剂盒检测碱性磷酸酶活性。3、分别将不同浓度的钛合金颗粒(1mg/ml，0.1mg/ml，0.01mg/ml)与成骨细胞基因培养3、6、9 天用RT-PCR 方法半定量测定骨保护素基因mRNA 的表达。

After feeding of 20 d, 40 d and 60 d, the activities of nitric oxide synthase, bacteriolytic, superoxide dismutase, alkaline phosphatase and acid phosphatase in the serum were determined and the relativities between them were also analyzed.

在饲料中添加不同剂量的嗜酸小球菌投喂凡纳滨对虾，并在投喂后的20、40和60d，分别测定凡纳滨对虾血清中的一氧化氮合酶、溶菌活力、总超氧化物歧化酶、酸性磷酸酶、碱性磷酸酶，并分析了这5种酶之间的相关性。

By n-butanol extraction, ammonium sulfate fractionation and sodium chloride dialyzation, the partially purified enzyme was gained by means of gel filtration on sephadex G-150 column, and some properties of the enzyme were determined.

方法］以DFRKN-1为提酶材料，经正丁醇提取，硫酸按分级沉淀分离，透析获得酶的粗提取液，用SephadexG-150凝胶过滤柱层析纯化，获得纯化碱性磷酸酶，并测定纯化碱性磷酸酶的理化性质。

Methods: Serum alcohol and ALT、AST、GGT、ALP、CK、LDH、AMY、LIPA measured before and after beer consuming (4ml/kg) in 17volunteers. Meanwhile, the direct inhibitory effects of beer on the serum enzymes were studied by comparing the serum enzyme activities with or without beer.

利用17名健康志愿者，观察口服啤酒(4ml/kg)前后不同时间血液中酒精浓度及丙氨酸氨基转移酶、天冬氨酸氨基转移酶、γ-谷氨酰转肽酶、碱性磷酸酶、肌酸激酶、乳酸脱氢酶、淀粉酶、脂肪酶活性的变化，同时在体外加入血清中与体内浓度相近的啤酒，以观察啤酒对酶活性的直接影响。

By means of the electron microscopic enzymo cytochemistry, it was observed that the activity of acid phosphatase was localized on the autophagic vacuole, the enzyme reaction granules gather to form the vacuolar bodies of various shapes in the cytoplasmic region of the osmiophilic and paraglycogen granules in resting Stylonychia mytilus .

该文应用电镜酶细胞化学方法显示，贻贝棘尾虫休眠包囊中，在胞质嗜锇粒和拟糖原粒区，酸性磷酸酶反应颗粒聚集于具有消化泡作用的泡状体上，葡萄糖 6 磷酸酶反应也定位在自噬泡膜位置。

Stabilized, hypophosphorylated β-catenin translocates to the nucleus, where it interacts with transcription factors of the TCF/LEF-1 family, leading to the increased expression of genes, such as c-myc and cyclin D1

那么，药物的作用有以下几个可能性：2。药物促进或抑制catenin的核转位，但不影响其磷酸化程度3。药物可直接磷酸化catenin或直接使其去磷酸化，即具有磷酸激酶或磷酸酶的作用。

objectiveto investigate the effects of yuegan capsule on ccl4 intoxicated primary cultured rat hepatocytes.methodshepatocytes injury models were induced by ccl4 in rats in vitro, and treated with yuegan capsule.structure of rat hepatocytes and effect of sdh、acpase、g 6 pase activeties and amount of hepatic glycogen were observed.

目的探讨悦肝胶囊对四氯化碳（ccl4）损伤原代培养大鼠肝细胞的保护作用。方法以ccl4诱导大鼠肝细胞损伤模型，观察悦肝胶囊对肝细胞的琥珀酸脱氢酶、酸性磷酸酶、葡萄糖-6-磷酸酶（g 6 pase）活性和糖原含量以及肝细胞形态结构的影响。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。