磷酸酶

The results showed: the calcium, phosphate and ALP in blood in the osteoporosis rats induced by DXM were normal, the bone ash wight, bone dense and intestinal Ca2+ absorption were declined compared with these of the normal rats (p.05). Afterwards, the osteoporosis rats were treated with replenishing kideny herbs or calcium.

实验结果发现，地塞米松诱发的骨质疏松大鼠的血钙、磷、碱性磷酸酶及尿羟脯氨酸均在正常水平，骨密度、骨灰重下降，肠钙吸收减少，与正常大鼠比较有统计学差异（P.05），血中雌二醇、1，25-2-D3水平降低（P.01，P.05），PGE2升高（P.05），经过补肾中药或钙剂治疗后，血雌二醇、1，25-2-D3水平回升而PGE2回降，均接近正常水平。

METHODS: The calvarium bone of new born rats was digested with bacterial collagenase and cultured to obtain osteoblasts and SP with different concentrations was then added.

采用新生大鼠颅盖骨消化法培养成骨细胞，再加入不同浓度的SP，观察细胞生长数量，测定碱性磷酸酶活性及观察细胞周期变化。

METHODS: The calvarium bone of newborn rats was digested with bacterial collagenase and cultured to obtain osteoblasts and SP with different concentrations was then added. The proliferation and activity changes of the osteoblasts were identified by counting the cells, determining the ALP activity and observing the changes of cell cycle.

采用新生大鼠颅盖骨消化法培养成骨细胞，再加入不同浓度的SP，观察细胞生长数量，测定碱性磷酸酶活性及观察细胞周期变化。

METHODS: calvarium bone of newborn rats was digested with bacterial collagenase and cultured to obtain osteoblasts and SP with different concentrations was then added. The proliferation and activity changes of the osteoblasts were identified by counting the cells, determining the ALP activity and observing the changes of cell cycle.

采用新生大鼠颅盖骨消化法培养成骨细胞，再加入不同浓度的SP，观察细胞生长数量，测定碱性磷酸酶活性及观察细胞周期变化。

The calvarium bone of newborn rats was digested with bacterial collagenase and cultured to obtain osteoblasts and SP with different concentrations was then added. The proliferation and activity changes of the osteoblasts were identified by counting the cells, determining the ALP activity and observing the changes of cell cycle.

采用新生大鼠颅盖骨消化法培养成骨细胞，再加入不同浓度的SP，观察细胞生长数量，测定碱性磷酸酶活性及观察细胞周期变化。

Sclerostin expression and distances of each osteocyte to the canal surface and cement line were assessed for all osteonal osteocytes in 636 unremodelled osteons chosen from fields ( approximately 0.5mm diameter) with at least one canal staining for alkaline phosphatase (ALP; a marker of bone formation).

我们评估了对636个未重塑的骨单位中所有的骨单位骨细胞的硬化蛋白表达和每个骨细胞到管道表面和粘合线的距离，这些骨单位从至少一个管道碱性磷酸酶(ALP；骨形成标志物)染色的区域选择（直径大约0.5mm）。

And the brains were got, post fixed, dehydrated gradiently in the alcohol, then impregnated andembedded in the celloidin, sliced into 90μm thick. The slices with the biggest dorsal hippocampus were stained by the alkali phosphatase method, then routine mounting.

对照组和实验组动物分别在给予生理盐水或KA7d后，水合氯醛麻醉，心脏灌流固定，取脑，后固定，酒精梯度脱水，火棉胶包埋，切片(厚90μm)，碱性磷酸酶染色，常规封片。

HGF can regulate the proliferation and differentiation of chondrocyte both in vivo and in vitro.

外源性HGF能加强骨、软骨的形态发生，提高培养软骨细胞Ⅱ型胶原mRNA的表达和碱性磷酸酶活性。

Constructed cell model under simulated weightlessness by clinostat and confirmed the credibility by assessing the expression of biomarkers—alkaline phosphatase and osteocalcin.

对成骨活性标志物碱性磷酸酶、骨钙素和体外钙化能力进行检测以确定模型的成立。

Model group and the normal group were taken distilled water,rats in the Tangshukang group were taken Tanggukang powder, western medicine control group were taken Calcium Carbonateand alpha D3. once a day for 12 weeks. Ca,P, BALP,BGP in blood and bone miner density;bone mineral contents, osseous vitodynamics,the content of Ca and P in the cremains were tested respectively.The femur was taken and detected the expression of TGF-β_1imRNA , the duodenum was taken and detected expression of VDR mRNA and CaBp-D9K mRNA with situ hybridization.

分别给予糖疏康、碳酸钙阿法迪三和生理盐水灌胃给药。12周后取材，测体重、血糖、血清钙、磷、血清骨源性碱性磷酸酶、骨T-羧基谷氨酸蛋白、骨密度、骨矿含量、骨的生物力学、骨干重、灰重、骨钙、磷等指标、组织病理切片骨形态计量学指标测定、并进行维生素D受体mRNA、转移生长因子-β_1(TGF-β_1)mRNA、钙结合蛋白(CaBp-D9K)mRNA原位杂交。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。