磷酸酶

The number of the cell adhered on the materials and the activity of ALP were calculated72hours after the culture.

培养72h后计算材料上所附着的细胞数以及细胞内碱性磷酸酶的活性。

Serum Ca, P, ALP and connective peptide in type 2 diabetes mellitus patients were measured.

测定2型糖尿病患者的血钙、磷、碱性磷酸酶及C肽水平。

MAIN OUTCOME MEASURES: Proliferation and osteogenic activity of cells were measured by morphology, ultrastructure, and cell proliferation curves, analyzed by calcification nodi Von Kossa staining, and determend by quantitative analysis of contents of cytoplasmic alkaline phosphatase and amendable calcium-cobalt staining.

主要观察指标：通过形态学、超微结构，细胞增殖曲线，钙结节Von kossa法染色以及细胞内碱性磷酸酶定量检测与钙钴法染色确定其增殖与成骨活性。

The cells were round before adherence,and anomalism fusiform,triangle and polygon when adhered,monocaryon,1-3 nucleole by invert phase contrast microscopy observation.Alkaline phosphatase staining and mineralized nodules staining were positive(≥98.06%).The osteoblastic activity was the highest on day 10,which was observed by MTT and morphological method.

倒置相差显微镜形态观察结果显示，未贴壁的细胞呈圆形，贴壁生长的细胞呈不规则梭形、三角形或多角形，单核，有1~3个核仁；碱性磷酸酶和钙化结节染色均呈阳性（阳性率≥98.06%）；MTT法结合形态学观察结果显示，成骨细胞培养至第10d时活性最强。

The part one of this thesis was concerned with expression and purification of recombinant low molecular weight sweet potato PAP and generation of a polyclonal antiserum from it.

本论文的第一部分致力于对低分子量的红薯紫色酸性磷酸酶的表达和纯化的研究，并产生相应的抗血清。

Topical calcineurin inhibitors have been added to the topical treatment armamentarium.

局部运用钙调神经磷酸酶抑制剂是局部治疗的新方法。

Serum alkaline phosphatase and aspartate aminotransferase were increased in 15 and 18 cases respectively.

血清碱性磷酸酶升高者为15例，天冬氨酸转氨酶升高者为8例。

Atypically, there is only a slight increase in the level of alkaline phosphatase.

碱性磷酸酶只有不典型的轻度升高。

Methods The immobilizing conditions of neutrophil alkaline phosphatase staining were detected by the stationary liquid of 40%, 60% and 80% acetone-citric acid, and at the same time, the staining conditions were detected at 10, 15 and 20 minutes in the matrix liquid prepared respectively with 2-amino-2-methyl-1.3-propylene glycol (PH9.4-9.6) buffer, barbital (PH9.2) buffer and Tris (PH9.2) buffer.

用40%、60%、80%的丙酮-枸缘酸固定液对中性粒细胞碱性磷酸酶染色固定条件进行测定。同时用2-氨基-2-甲基-1.3-丙二醇(pH9.4-9.6)缓冲液、巴比妥(pH9.2)缓冲液、Tris(pH9.2)缓冲液配制的基质液分别在10、15、20分钟的染色条件进行测定。

Experiments on decalcification and activation of osteoinductive ability, comparison between the osteogenesis of demineralized bone matrix in orthotopic and in heterotopic sites, and correlation between immune response to allograft of bone and osteoinductive activity of the graft, were reported in this paper. 1. Syngeneic deminerolized bone matrix and the mixture of DBM with bone ash or acid-soluble protein extracted from demineralization was implanted into muscle pouch of mice, subsequently, the inhibition of DBM osteoinduction by bone ash or acid-soluble protein was demonstrated with histological and by means of alkaline phosphatase assay.

本文对脱钙处理使骨诱导活性表达的作用、脱钙骨基质植入不同部位的成骨表现，以及同种异体骨移植引发的免疫反应与骨诱导活性的内在关系三方面进行了研究：（1）在小鼠肌肉植入同系脱钙骨基质及其与脱钙所去除的骨矿盐和酸溶性蛋白混合物，碱性磷酸酶和组织学检查表明：骨矿盐和酸溶性蛋白具有抑制脱钙骨基质骨诱导活性的作用。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。